FastQCFastQC Report
Thu 26 May 2016
SRR1545303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545303_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2290835
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45570.19892310009232442No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28250.12331748030739882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG351.2126293E-745.0000041
ATAGCGC351.2126293E-745.00000434
CAGTCGA351.2126293E-745.00000426
AGTCGAT351.2126293E-745.00000427
TCGCCTC207.0347567E-445.012
AGCCGTG253.8920243E-545.031
TGCCCGT207.0347567E-445.013
CGAGTGA253.8920243E-545.042
GACGTGA453.8562575E-1045.09
TATATCG207.0347567E-445.01
ATTCGCA253.8920243E-545.011
TCGATTG502.1827873E-1145.01
TACGGAC453.8562575E-1045.025
CGTTATC253.8920243E-545.034
CCGTATT207.0347567E-445.024
ATATCGA207.0347567E-445.015
CCCGTCG551.8189894E-1245.023
CCCGTAG253.8920243E-545.013
CCGTAAG253.8920243E-545.01
CGCTATA253.8920243E-545.033