##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545303_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2290835
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239854027025082	31.0	31.0	33.0	30.0	34.0
2	31.5462724290488	31.0	31.0	34.0	30.0	34.0
3	31.625172917298713	31.0	31.0	34.0	30.0	34.0
4	30.233934787970327	35.0	33.0	37.0	0.0	37.0
5	32.78752332664727	35.0	33.0	37.0	19.0	37.0
6	34.38751459620619	35.0	33.0	37.0	30.0	37.0
7	35.49396355477369	36.0	35.0	37.0	33.0	37.0
8	35.70257657142483	37.0	35.0	37.0	35.0	37.0
9	37.74747548383013	39.0	37.0	39.0	35.0	39.0
10	37.14813113995552	39.0	37.0	39.0	33.0	39.0
11	36.601133211252666	39.0	35.0	39.0	32.0	39.0
12	35.25201902363112	35.0	35.0	39.0	31.0	39.0
13	34.69868672339998	35.0	33.0	39.0	28.0	39.0
14	35.42520652949688	36.0	33.0	40.0	27.0	41.0
15	35.85951978208819	36.0	34.0	40.0	30.0	41.0
16	36.12086291679671	36.0	34.0	40.0	31.0	41.0
17	36.067346185997685	36.0	34.0	40.0	31.0	41.0
18	36.06409278712784	36.0	35.0	40.0	31.0	41.0
19	36.032090918813445	36.0	35.0	40.0	31.0	41.0
20	35.91308845901167	36.0	34.0	40.0	31.0	41.0
21	35.723506057834804	35.0	34.0	40.0	30.0	41.0
22	35.57236990005828	35.0	34.0	40.0	30.0	41.0
23	35.55819821156914	35.0	34.0	40.0	30.0	41.0
24	35.48126862039388	35.0	34.0	40.0	30.0	41.0
25	35.36649082103251	35.0	34.0	40.0	30.0	41.0
26	35.20884611942807	35.0	34.0	40.0	29.0	41.0
27	35.18783674948217	35.0	34.0	40.0	29.0	41.0
28	35.268048549982865	36.0	34.0	40.0	29.0	41.0
29	35.37572937378729	36.0	34.0	40.0	30.0	41.0
30	35.333162362195445	36.0	34.0	40.0	30.0	41.0
31	35.17421944400186	35.0	34.0	40.0	29.0	41.0
32	35.05607475003656	35.0	34.0	40.0	29.0	41.0
33	34.921148838742205	35.0	34.0	40.0	29.0	41.0
34	34.87884024820644	35.0	34.0	40.0	29.0	41.0
35	34.75999493634417	35.0	34.0	40.0	29.0	41.0
36	34.53593078506309	35.0	33.0	40.0	27.0	41.0
37	34.44276999434704	35.0	33.0	40.0	27.0	41.0
38	34.45217748113679	35.0	33.0	39.0	27.0	41.0
39	34.460668708134804	35.0	33.0	39.0	27.0	41.0
40	34.34950793051442	35.0	33.0	39.0	26.0	41.0
41	34.40515401589377	35.0	33.0	39.0	27.0	41.0
42	34.34956729751379	35.0	33.0	39.0	27.0	41.0
43	34.23987803573806	35.0	33.0	39.0	27.0	41.0
44	34.17889503172424	35.0	33.0	39.0	27.0	41.0
45	34.10178821259497	35.0	33.0	39.0	27.0	41.0
46	34.094630996994546	35.0	33.0	39.0	27.0	41.0
47	34.030261454884354	35.0	33.0	39.0	26.0	41.0
48	33.96229540756973	35.0	33.0	39.0	26.0	41.0
49	33.98317687655375	35.0	33.0	38.0	26.0	40.0
50	33.77820838253301	35.0	33.0	38.0	26.0	40.0
51	33.69718596057769	35.0	33.0	38.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	7.0
14	7.0
15	36.0
16	131.0
17	403.0
18	1029.0
19	1981.0
20	3626.0
21	5901.0
22	9004.0
23	13435.0
24	18969.0
25	26308.0
26	34013.0
27	41717.0
28	50355.0
29	61503.0
30	75807.0
31	95991.0
32	124270.0
33	184467.0
34	282064.0
35	393823.0
36	152518.0
37	180556.0
38	237805.0
39	294911.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.61049791888111	26.44459334696737	31.774309367545023	11.170599366606499
2	27.180002051653656	26.4510102211639	32.39796842635982	13.971019300822626
3	27.630143593929724	25.8803012875218	31.87597535396482	14.613579764583656
4	21.277176226135886	39.01769442146641	27.454094249476718	12.251035102920987
5	23.086210923091365	32.26971824684012	30.57762780820094	14.066443021867572
6	21.456368529379024	39.988955992029105	26.762425054619822	11.792250423972044
7	89.95418701041324	4.212612431711581	4.152852562493589	1.6803479953815967
8	92.38592041766431	2.3466552588903173	3.366021559824256	1.901402763621125
9	89.60370345310771	3.826377718168266	4.606617237819398	1.9633015909046265
10	62.76418860371873	21.744996911606467	7.784192226851781	7.70662225782302
11	55.02513275726974	15.9732586589606	18.257665872924065	10.743942710845609
12	49.01090650352383	21.76987866869504	17.514443423467863	11.704771404313274
13	19.01258711343244	49.3539691859082	19.16921122647419	12.464232474185177
14	12.33091863883693	48.99410040443769	24.625387686149374	14.049593270576013
15	10.940115721996564	22.19679723768844	54.28169204678643	12.58139499352856
16	12.805243502914877	16.68880561018144	51.30395685416016	19.20199403274352
17	14.004718803405744	18.33060870817846	26.188354901160494	41.4763175872553
18	21.366750551654746	23.409018982161527	34.160164306901194	21.064066159282532
19	31.766190057337173	25.386114669978415	21.938943660281076	20.908751612403336
20	34.01742159518255	24.131506634043916	23.0380625405147	18.813009230258835
21	21.12029019986162	30.391276543269157	26.169235235187173	22.319198021682052
22	22.85162397117208	25.62410649392034	22.79793175850727	28.726337776400307
23	18.997265189330527	33.32579605253107	22.760609122874413	24.916329635264
24	20.070192746313026	23.057531424131376	40.97676174844543	15.895514081110163
25	15.293375559566707	26.91918012427783	36.599013023635486	21.188431292519976
26	14.704681917292167	40.389988803209306	24.412714141350207	20.492615138148317
27	16.252763730255563	37.59851757110398	28.00389377672333	18.14482492191712
28	12.533988698444018	29.783201321788784	41.44362208539681	16.239187894370392
29	12.445898547909387	25.229708817963754	39.50899999345217	22.815392640674688
30	17.915519886853485	33.25887722162443	29.88787931038246	18.93772358113963
31	31.389995351040124	28.244897602839142	21.09239644059917	19.272710605521567
32	32.53508000357948	27.97696036598009	23.043955588246206	16.444004042194223
33	28.479964729018022	28.41627616131236	22.111937350354783	20.991821759314835
34	17.11493843947731	28.353329681098817	27.71513443787964	26.816597441544243
35	19.117919885107394	25.75100345507206	31.28418240510556	23.846894254714986
36	33.14105991919977	23.119473903620296	25.346260206431282	18.393205970748657
37	19.44286690224307	33.278258800830265	29.76364513376127	17.515229163165397
38	19.07444228851052	35.103182900558096	22.72542544530706	23.096949365624326
39	19.652266531635846	34.53605344776031	26.22301475226282	19.588665268341018
40	25.064572524865387	25.269563281510894	23.43844056861363	26.227423625010093
41	15.870414062994499	23.028590011938878	26.874785831367166	34.226210093699464
42	22.154454598432448	24.81186990769741	22.716564047607097	30.317111446263045
43	22.103119604860236	26.604185809977583	25.11555830079425	26.177136284367926
44	18.747792835363526	32.164734692808516	27.171227958364526	21.916244513463432
45	15.304899741797204	41.1816652006801	19.919548985413616	23.593886072109076
46	22.133283278804452	34.61938550790432	23.930837445734852	19.316493767556373
47	20.306569438654464	26.774385758904508	27.800212586240384	25.118832216200644
48	22.97856458452922	21.856179078807507	32.807382460980385	22.357873875682884
49	20.38610375692706	22.50650963513304	32.573755857580316	24.533630750359585
50	18.592129070840983	35.424070262589844	25.201727754290467	20.78207291227871
51	16.530129843485017	35.94920629377498	22.48725901254346	25.033404850196543
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	403.0
1	632.0
2	861.0
3	2989.5
4	5118.0
5	3773.5
6	2429.0
7	2844.0
8	3259.0
9	3831.5
10	4404.0
11	4644.0
12	4884.0
13	4910.5
14	4937.0
15	4903.0
16	4869.0
17	4617.5
18	4366.0
19	4296.0
20	4226.0
21	4719.5
22	5213.0
23	5294.5
24	5376.0
25	7425.0
26	13381.5
27	17289.0
28	20316.0
29	23343.0
30	27089.5
31	30836.0
32	35785.5
33	40735.0
34	48182.5
35	55630.0
36	61380.5
37	67131.0
38	89894.0
39	112657.0
40	158554.0
41	204451.0
42	247092.0
43	289733.0
44	284826.5
45	279920.0
46	259136.5
47	238353.0
48	217937.0
49	197521.0
50	187042.0
51	176563.0
52	151887.5
53	127212.0
54	111657.0
55	96102.0
56	83758.5
57	71415.0
58	65578.0
59	59741.0
60	53978.0
61	48215.0
62	42327.0
63	36439.0
64	29144.0
65	21849.0
66	17485.5
67	13122.0
68	11395.5
69	9669.0
70	7649.0
71	5629.0
72	4493.0
73	3357.0
74	2775.5
75	1784.0
76	1374.0
77	841.5
78	309.0
79	215.0
80	121.0
81	85.5
82	50.0
83	46.5
84	43.0
85	27.5
86	12.0
87	6.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2290835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.360431816822132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91593026309349	13.352937629598804
2	10.154326533496766	3.5256698686103682
3	3.708372135177418	1.9313682481245202
4	1.8264130454382248	1.2682927657873904
5	1.0117428297764008	0.8782146206245943
6	0.6256970954494954	0.6517423058120768
7	0.4343242198972411	0.5278039204144361
8	0.2989907015192772	0.4152486150071383
9	0.25037830270515105	0.39120079072719843
>10	2.2269189983398516	9.057685157908557
>50	0.8877292417230108	11.260969444477444
>100	1.6379365583140368	53.73172286911245
>500	0.01851698852227034	2.088516205384306
>1k	0.002723086547392697	0.9186275584107343
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4557	0.19892310009232442	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2825	0.12331748030739882	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09594754750996907	0.0
2	0.0	0.0	0.0	0.35480512564196026	0.0
3	0.0	0.0	0.0	0.510992716629526	0.0
4	0.0	0.0	0.0	0.7729932535516526	0.0
5	0.0	0.0	0.0	1.2761722254112584	0.0
6	0.0	0.0	0.0	1.7346513389222706	0.0
7	0.0	0.0	0.0	1.981897430412928	0.0
8	0.0	0.0	0.0	2.566400460967289	0.0
9	0.0	0.0	0.0	2.7477753744813573	0.0
10	0.0	0.0	0.0	3.0515510719890346	0.0
11	0.0	0.0	0.0	3.556912654119568	0.0
12	0.0	0.0	0.0	3.9297461405993883	0.0
13	0.0	0.0	0.0	4.078163639022453	0.0
14	0.0	0.0	0.0	4.136832203104982	0.0
15	0.0	0.0	0.0	4.23160114106865	0.0
16	0.0	0.0	0.0	4.468763573107623	0.0
17	0.0	0.0	0.0	4.763939786147846	0.0
18	0.0	0.0	0.0	5.088712194461845	0.0
19	0.0	0.0	0.0	5.28789720778668	0.0
20	0.0	0.0	0.0	5.471367427160839	0.0
21	0.0	0.0	0.0	5.740483273566189	0.0
22	0.0	0.0	0.0	6.025357566127635	0.0
23	0.0	0.0	0.0	6.339435184114089	0.0
24	0.0	0.0	0.0	6.561450300872826	0.0
25	0.0	0.0	0.0	6.740031473240107	0.0
26	0.0	0.0	0.0	6.911715597151257	0.0
27	0.0	0.0	0.0	7.088245115863867	0.0
28	0.0	0.0	0.0	7.270973247745909	0.0
29	0.0	0.0	0.0	7.471555131644139	0.0
30	0.0	0.0	0.0	7.726614967904716	0.0
31	0.0	0.0	0.0	7.955745394146676	0.0
32	0.0	0.0	0.0	8.148295272247893	0.0
33	0.0	0.0	0.0	8.349444634816562	0.0
34	0.0	0.0	0.0	8.574559058160016	0.0
35	4.365220541854826E-5	0.0	0.0	8.857032479423442	0.0
36	4.365220541854826E-5	0.0	0.0	9.083980295394474	0.0
37	4.365220541854826E-5	0.0	0.0	9.312936112814759	0.0
38	4.365220541854826E-5	0.0	0.0	9.539054536882839	0.0
39	4.365220541854826E-5	0.0	0.0	9.78529662764887	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	35	1.2126293E-7	45.000004	1
ATAGCGC	35	1.2126293E-7	45.000004	34
CAGTCGA	35	1.2126293E-7	45.000004	26
AGTCGAT	35	1.2126293E-7	45.000004	27
TCGCCTC	20	7.0347567E-4	45.0	12
AGCCGTG	25	3.8920243E-5	45.0	31
TGCCCGT	20	7.0347567E-4	45.0	13
CGAGTGA	25	3.8920243E-5	45.0	42
GACGTGA	45	3.8562575E-10	45.0	9
TATATCG	20	7.0347567E-4	45.0	1
ATTCGCA	25	3.8920243E-5	45.0	11
TCGATTG	50	2.1827873E-11	45.0	1
TACGGAC	45	3.8562575E-10	45.0	25
CGTTATC	25	3.8920243E-5	45.0	34
CCGTATT	20	7.0347567E-4	45.0	24
ATATCGA	20	7.0347567E-4	45.0	15
CCCGTCG	55	1.8189894E-12	45.0	23
CCCGTAG	25	3.8920243E-5	45.0	13
CCGTAAG	25	3.8920243E-5	45.0	1
CGCTATA	25	3.8920243E-5	45.0	33
>>END_MODULE