FastQCFastQC Report
Thu 26 May 2016
SRR1545302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545302_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2217514
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30770.13875898866929365No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG207.0347084E-445.039
ACGTTAC502.1827873E-1145.029
TACGCTA351.2126111E-745.01
TACGACA406.8193913E-945.043
ACTAACG700.045.01
ACTCGCT351.2126111E-745.015
ATGGTCG207.0347084E-445.044
CGTCGGT351.2126111E-745.017
TTACGCG406.8193913E-945.01
CCGAATA351.2126111E-745.040
CACCGTA207.0347084E-445.038
TGTAACG700.045.01
TATCGCC502.1827873E-1145.038
TACGTCG406.8193913E-945.026
TCTATCG207.0347084E-445.032
CGCTAAT302.1664182E-644.99999619
TATTACG302.1664182E-644.9999961
GAACCGT302.1664182E-644.99999617
GCGTAAG1300.044.9999961
ACGTAAG1950.042.692311
Produced by FastQC (version 0.11.5)