##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545302_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2217514
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325547888310965	31.0	31.0	33.0	30.0	34.0
2	31.648952836374427	31.0	31.0	34.0	30.0	34.0
3	31.750865158010278	31.0	31.0	34.0	30.0	34.0
4	30.306051731804175	35.0	33.0	37.0	0.0	37.0
5	32.90082633074695	35.0	33.0	37.0	19.0	37.0
6	34.5496898779444	35.0	33.0	37.0	30.0	37.0
7	35.517286474854274	36.0	35.0	37.0	33.0	37.0
8	35.71077251372483	37.0	35.0	37.0	35.0	37.0
9	37.76052597638617	39.0	37.0	39.0	35.0	39.0
10	37.20831661040246	39.0	37.0	39.0	33.0	39.0
11	36.754475507257226	39.0	35.0	39.0	32.0	39.0
12	35.70820883205247	37.0	35.0	39.0	32.0	39.0
13	35.30684180573381	37.0	35.0	39.0	30.0	39.0
14	36.137890899448664	38.0	35.0	40.0	31.0	41.0
15	36.45225419095438	38.0	35.0	40.0	31.0	41.0
16	36.636012670044025	38.0	35.0	40.0	32.0	41.0
17	36.57771585658535	38.0	35.0	40.0	32.0	41.0
18	36.588172160356145	37.0	35.0	40.0	32.0	41.0
19	36.559612701430524	37.0	35.0	40.0	31.0	41.0
20	36.45131710555153	37.0	35.0	40.0	31.0	41.0
21	36.29170638832495	37.0	35.0	40.0	31.0	41.0
22	36.158942852221	37.0	35.0	40.0	31.0	41.0
23	36.1018049942413	37.0	34.0	40.0	31.0	41.0
24	36.04334809160168	37.0	34.0	40.0	31.0	41.0
25	35.91209660908567	36.0	34.0	40.0	31.0	41.0
26	35.79129962651871	36.0	34.0	40.0	30.0	41.0
27	35.786795032635645	36.0	34.0	40.0	30.0	41.0
28	35.81909065737578	36.0	34.0	40.0	30.0	41.0
29	35.853452108983305	36.0	34.0	40.0	30.0	41.0
30	35.81833034650514	36.0	34.0	40.0	30.0	41.0
31	35.694302268215665	36.0	34.0	40.0	30.0	41.0
32	35.56662280373427	36.0	34.0	40.0	30.0	41.0
33	35.43935866921246	36.0	34.0	40.0	30.0	41.0
34	35.37169686414607	36.0	34.0	40.0	29.0	41.0
35	35.23826320825934	36.0	34.0	40.0	29.0	41.0
36	35.08083827204699	36.0	34.0	40.0	28.0	41.0
37	35.03584960455718	36.0	34.0	40.0	28.0	41.0
38	35.01513316263167	35.0	34.0	40.0	28.0	41.0
39	34.97263782776569	35.0	34.0	40.0	28.0	41.0
40	34.868709284360776	35.0	34.0	40.0	27.0	41.0
41	34.86222183941116	35.0	34.0	40.0	28.0	41.0
42	34.796382796230375	35.0	34.0	40.0	27.0	41.0
43	34.714114093529965	35.0	33.0	40.0	27.0	41.0
44	34.64442479280852	35.0	33.0	40.0	27.0	41.0
45	34.54928221422728	35.0	33.0	40.0	27.0	41.0
46	34.5028545479307	35.0	33.0	39.0	27.0	41.0
47	34.42547014359323	35.0	33.0	39.0	27.0	41.0
48	34.34788326026352	35.0	33.0	39.0	27.0	41.0
49	34.31158315122249	35.0	33.0	39.0	27.0	41.0
50	34.14309582712894	35.0	33.0	39.0	26.0	41.0
51	34.050538576081145	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	4.0
14	8.0
15	31.0
16	86.0
17	247.0
18	641.0
19	1289.0
20	2556.0
21	4303.0
22	6757.0
23	10388.0
24	15112.0
25	20979.0
26	27882.0
27	35065.0
28	42664.0
29	52246.0
30	66510.0
31	84551.0
32	110888.0
33	163453.0
34	250190.0
35	344419.0
36	166565.0
37	205777.0
38	269562.0
39	335120.0
40	219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.789472355078708	25.186537717461988	31.29215869663055	11.731831230828757
2	28.01736539205615	26.752255002674165	31.34023054645878	13.890149058810902
3	28.40076770653985	26.63203028255966	30.872815233635503	14.094386777264992
4	22.320896282954696	38.548212096969856	26.093679679136184	13.037211940939267
5	24.673079854287277	32.60060590372823	29.025340989955417	13.700973252029074
6	21.8894672141867	41.59581405123034	24.963269679469892	11.551449055113068
7	90.40493092715536	4.115419338953441	3.7155571509356875	1.7640925829555079
8	92.387827089254	2.3443369466889497	3.4064722928468543	1.8613636712101929
9	88.66216853647823	4.482497066534868	4.569035415334469	2.286298981652427
10	57.62078615963642	25.119615930271465	8.730948260078629	8.528649650013485
11	49.86146648905035	19.60821893345431	17.76602086841391	12.764293709081432
12	45.58970089929534	22.19020939664868	18.737063215835388	13.483026488220592
13	21.65469981249273	43.02795833532505	19.732231679258845	15.585110172923372
14	14.841845417886876	43.995167561512574	24.559574370218183	16.603412650382367
15	14.04920104224821	23.841382737606164	47.11821436076615	14.991201859379466
16	16.20066434755316	19.08064616502985	45.500456817860005	19.21823266955699
17	16.917142349495876	20.297594513495742	26.310093194451085	36.475169942557294
18	21.870842754544054	24.788253873481747	31.29017449269768	22.050728879276523
19	31.633982919611782	25.44746053463473	21.695421088660545	21.223135457092944
20	34.763433286103265	24.215224796776933	22.0421156303861	18.979226286733702
21	24.119757530279404	29.17550915123873	24.955332863738402	21.749400454743466
22	24.10947574626361	25.95036604053007	22.540286104168903	27.399872109037414
23	22.256950801663482	31.473848643120178	22.721795668482812	23.54740488673352
24	21.823176764611183	23.044454285294254	37.07002526252371	18.062343687570856
25	18.08818343424213	26.956222147864683	33.20691549185259	21.7486789260406
26	17.738828255424767	38.34564291364113	23.535634949768074	20.379893881166026
27	19.25967547442767	35.54439791586434	27.24415719585085	17.95176941385714
28	15.983303825815756	29.297492597566468	37.50993229355035	17.209271283067434
29	16.496355829095105	25.96479661458733	35.72072149262643	21.818126063691142
30	20.388552225600378	32.0811954287549	27.76735569651421	19.76289664913051
31	31.164357925135988	27.427921537361204	21.437294195211397	19.970426342291415
32	32.82978145797501	27.4235472696001	22.233501118820445	17.51317015360444
33	29.427367764081758	28.183542471434226	22.27562035685006	20.113469407633954
34	19.716448238883725	27.543366129819248	27.319692231931793	25.420493399365235
35	22.01659155252233	25.021082166786773	28.98921945926835	23.973106821422547
36	30.90875638214686	26.003308209102627	23.43529736452622	19.6526380442243
37	22.13695155926862	32.622928197973046	26.823100102186505	18.41702014057183
38	21.265344886210414	33.33782785587825	23.03651746956276	22.360309788348573
39	21.82876861205837	32.350956972537716	25.046155289211253	20.774119126192662
40	26.349010648861743	25.609714301690996	23.732927954457107	24.308347094990157
41	17.93621145120166	24.149791162536065	26.383779313230942	31.530218073031328
42	22.71895464921529	25.52827174935536	22.79142318830907	28.96135041312028
43	22.42700609781945	27.10323362107297	23.97590274514614	26.49385753596144
44	20.50228318738912	31.37012889208366	26.306530646480695	21.821057274046524
45	18.402273897707072	37.23394756470534	20.91035276440194	23.45342577318565
46	21.999725819092912	34.03441872294831	23.273404361821388	20.692451096137386
47	22.03751588490535	26.91577144496044	26.20650873004635	24.840203940087864
48	24.287152189343562	21.97140581750555	30.880346189471634	22.861095803679255
49	21.067059779554942	24.535944305199425	31.097751806752967	23.299244108492665
50	19.016069346123633	33.855614891270136	25.20484650829713	21.923469254309104
51	18.009221136822585	34.56136015375776	22.92562752704154	24.503791182378105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	352.0
1	723.0
2	1094.0
3	3534.5
4	5975.0
5	4370.0
6	2765.0
7	3073.5
8	3382.0
9	3684.5
10	3987.0
11	4234.5
12	4482.0
13	4360.5
14	4239.0
15	4241.0
16	4243.0
17	4122.0
18	4001.0
19	4570.5
20	5140.0
21	5716.5
22	6293.0
23	6609.5
24	6926.0
25	7803.5
26	11460.5
27	14240.0
28	18650.0
29	23060.0
30	28127.0
31	33194.0
32	37856.5
33	42519.0
34	48217.0
35	53915.0
36	56572.0
37	59229.0
38	77856.0
39	96483.0
40	134482.0
41	172481.0
42	203011.0
43	233541.0
44	235435.5
45	237330.0
46	221178.0
47	205026.0
48	189787.0
49	174548.0
50	164283.0
51	154018.0
52	141042.5
53	128067.0
54	115958.5
55	103850.0
56	96640.5
57	89431.0
58	87595.5
59	85760.0
60	80232.5
61	74705.0
62	67514.5
63	60324.0
64	48183.5
65	36043.0
66	31716.0
67	27389.0
68	23275.5
69	19162.0
70	15685.0
71	12208.0
72	10203.5
73	8199.0
74	6886.5
75	4269.5
76	2965.0
77	2159.0
78	1353.0
79	1148.0
80	943.0
81	611.0
82	279.0
83	156.5
84	34.0
85	35.0
86	36.0
87	34.0
88	32.0
89	21.5
90	11.0
91	7.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2217514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.914940031180695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70899306074104	13.921519505666124
2	9.442438886657104	3.3832145280510124
3	3.2366015000745505	1.7395056533599518
4	1.4596971283172304	1.0460154606995942
5	0.8279882905050596	0.7416680285458981
6	0.5649413765316909	0.6072534529018749
7	0.40307772315336327	0.505477926673815
8	0.3130000188263765	0.44859012536263715
9	0.25200556378130945	0.40631981063994366
>10	2.9659193239999047	12.785992952908565
>50	1.2506515450777425	16.225395892228452
>100	1.5623490831699867	46.37138158354143
>500	0.009869199331692927	1.069726103616932
>1k	0.0024672998329232317	0.7479389758037858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3077	0.13875898866929365	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08744025967818016	0.0
2	0.0	0.0	0.0	0.2791414169200285	0.0
3	0.0	0.0	0.0	0.3923312321816232	0.0
4	0.0	0.0	0.0	0.570413535156937	0.0
5	0.0	0.0	0.0	0.969238525664325	0.0
6	0.0	0.0	0.0	1.3117391818044892	0.0
7	0.0	0.0	0.0	1.5056049251549257	0.0
8	4.509554392892221E-5	0.0	0.0	1.956379982268432	0.0
9	4.509554392892221E-5	0.0	0.0	2.0876982061894536	0.0
10	4.509554392892221E-5	0.0	0.0	2.337347137379967	0.0
11	4.509554392892221E-5	0.0	0.0	2.7620118745586275	0.0
12	4.509554392892221E-5	0.0	0.0	3.0866095997590093	0.0
13	9.019108785784442E-5	0.0	0.0	3.2202276964204057	0.0
14	9.019108785784442E-5	0.0	0.0	3.277949992649426	0.0
15	9.019108785784442E-5	0.0	0.0	3.367915602787626	0.0
16	9.019108785784442E-5	0.0	0.0	3.580586187956423	0.0
17	9.019108785784442E-5	0.0	0.0	3.840832571970233	0.0
18	9.019108785784442E-5	0.0	0.0	4.1175388295181	0.0
19	9.019108785784442E-5	0.0	0.0	4.303648139312762	0.0
20	1.3528663178676663E-4	0.0	0.0	4.47695031463161	0.0
21	1.3528663178676663E-4	0.0	0.0	4.718436952370989	0.0
22	1.3528663178676663E-4	0.0	0.0	4.987116203099507	0.0
23	1.3528663178676663E-4	0.0	0.0	5.28781329001756	0.0
24	1.3528663178676663E-4	0.0	0.0	5.50373075434924	0.0
25	1.3528663178676663E-4	0.0	0.0	5.6792877068645335	0.0
26	1.3528663178676663E-4	0.0	0.0	5.846592174840835	0.0
27	1.3528663178676663E-4	0.0	0.0	6.010965432461757	0.0
28	1.3528663178676663E-4	0.0	0.0	6.189633977508146	0.0
29	1.3528663178676663E-4	0.0	0.0	6.376780484813174	0.0
30	1.3528663178676663E-4	0.0	0.0	6.613712472615731	0.0
31	1.3528663178676663E-4	0.0	0.0	6.834364969059948	0.0
32	1.3528663178676663E-4	0.0	0.0	7.036167528141874	0.0
33	1.3528663178676663E-4	0.0	0.0	7.238375947119161	0.0
34	1.3528663178676663E-4	0.0	0.0	7.458216723772657	0.0
35	1.3528663178676663E-4	0.0	0.0	7.731631006613712	0.0
36	1.3528663178676663E-4	0.0	0.0	7.9722157334745125	0.0
37	1.3528663178676663E-4	0.0	0.0	8.203510778285954	0.0
38	1.8038217571568883E-4	0.0	0.0	8.428176778139845	0.0
39	1.8038217571568883E-4	0.0	0.0	8.66393628180025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.0347084E-4	45.0	39
ACGTTAC	50	2.1827873E-11	45.0	29
TACGCTA	35	1.2126111E-7	45.0	1
TACGACA	40	6.8193913E-9	45.0	43
ACTAACG	70	0.0	45.0	1
ACTCGCT	35	1.2126111E-7	45.0	15
ATGGTCG	20	7.0347084E-4	45.0	44
CGTCGGT	35	1.2126111E-7	45.0	17
TTACGCG	40	6.8193913E-9	45.0	1
CCGAATA	35	1.2126111E-7	45.0	40
CACCGTA	20	7.0347084E-4	45.0	38
TGTAACG	70	0.0	45.0	1
TATCGCC	50	2.1827873E-11	45.0	38
TACGTCG	40	6.8193913E-9	45.0	26
TCTATCG	20	7.0347084E-4	45.0	32
CGCTAAT	30	2.1664182E-6	44.999996	19
TATTACG	30	2.1664182E-6	44.999996	1
GAACCGT	30	2.1664182E-6	44.999996	17
GCGTAAG	130	0.0	44.999996	1
ACGTAAG	195	0.0	42.69231	1
>>END_MODULE