FastQCFastQC Report
Thu 26 May 2016
SRR1545301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545301_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5567757
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61180.10988266908918617No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT55900.10039949660159378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG1650.042.2727281
TACGGGA12650.041.2648244
CGTAAGG7900.041.0126572
TACGTAA501.0822987E-940.51
ATCGGAT950.040.26315720
GCGAATG7000.039.8571431
TGTTACG4350.039.8275871
TATACGG4500.039.5000042
CGTTAGG9300.039.193552
TAAACGG5750.039.1304362
TTGTACG1150.039.1304361
CGTTTTT52800.039.0767021
ATAGGGC26750.038.8598144
ATGCACG700.038.5714261
TCGTCCC21200.038.52594438
TCGATTG2600.038.0769271
AATACGG4350.037.758622
TTAGGGA41050.037.7101064
CGTTGAT11700.037.69230725
TATGGGC14150.037.6855134
Produced by FastQC (version 0.11.5)