##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545301_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5567757
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243575464949352	31.0	31.0	33.0	30.0	34.0
2	31.561022688310572	31.0	31.0	34.0	30.0	34.0
3	31.66555383074369	31.0	31.0	34.0	30.0	34.0
4	30.301013316493517	35.0	33.0	37.0	0.0	37.0
5	32.84854924523466	35.0	33.0	37.0	19.0	37.0
6	34.45704975989433	35.0	33.0	37.0	30.0	37.0
7	35.48113611998512	36.0	35.0	37.0	33.0	37.0
8	35.67419249798438	37.0	35.0	37.0	35.0	37.0
9	37.70758565792293	39.0	37.0	39.0	35.0	39.0
10	37.11329445591824	39.0	37.0	39.0	33.0	39.0
11	36.67636446777401	39.0	35.0	39.0	32.0	39.0
12	35.68956062558046	37.0	35.0	39.0	31.0	39.0
13	35.25420613722905	37.0	35.0	39.0	30.0	39.0
14	35.98217989757814	38.0	35.0	40.0	30.0	41.0
15	36.310790503249336	38.0	35.0	40.0	31.0	41.0
16	36.48789377841023	38.0	35.0	40.0	31.0	41.0
17	36.4348706310279	38.0	35.0	40.0	31.0	41.0
18	36.426241123669726	38.0	35.0	40.0	31.0	41.0
19	36.42362175648111	38.0	35.0	40.0	31.0	41.0
20	36.32541578233389	38.0	35.0	40.0	31.0	41.0
21	36.174360878177694	37.0	34.0	40.0	30.0	41.0
22	36.09550470683257	37.0	34.0	40.0	30.0	41.0
23	36.057618175505866	37.0	34.0	40.0	30.0	41.0
24	35.977177703696476	37.0	34.0	40.0	30.0	41.0
25	35.86133213069464	37.0	34.0	40.0	30.0	41.0
26	35.72439996932337	37.0	34.0	40.0	30.0	41.0
27	35.7378403906636	37.0	34.0	40.0	30.0	41.0
28	35.754903994552926	37.0	34.0	40.0	30.0	41.0
29	35.75954482208904	37.0	34.0	40.0	30.0	41.0
30	35.67903951267988	36.0	34.0	40.0	30.0	41.0
31	35.542818948456265	36.0	34.0	40.0	29.0	41.0
32	35.47269448002131	36.0	34.0	40.0	29.0	41.0
33	35.350100767687955	36.0	34.0	40.0	29.0	41.0
34	35.218168106115264	36.0	34.0	40.0	28.0	41.0
35	35.106449329595385	36.0	34.0	40.0	28.0	41.0
36	34.976354751114314	36.0	34.0	40.0	27.0	41.0
37	34.9099240502055	36.0	33.0	40.0	27.0	41.0
38	34.87700415086363	36.0	33.0	40.0	27.0	41.0
39	34.805806000513314	36.0	33.0	40.0	27.0	41.0
40	34.660551277650946	35.0	33.0	40.0	26.0	41.0
41	34.61611992046348	35.0	33.0	40.0	26.0	41.0
42	34.56961807061623	35.0	33.0	40.0	26.0	41.0
43	34.499822639529704	35.0	33.0	40.0	26.0	41.0
44	34.45881492313691	35.0	33.0	40.0	26.0	41.0
45	34.37775463261058	35.0	33.0	40.0	26.0	41.0
46	34.30890033455124	35.0	33.0	39.0	26.0	41.0
47	34.236084297500774	35.0	33.0	39.0	26.0	41.0
48	34.12454530612597	35.0	33.0	39.0	26.0	41.0
49	34.09534306184699	35.0	33.0	39.0	26.0	41.0
50	33.940392154327135	35.0	33.0	39.0	25.0	41.0
51	33.86188244206778	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	4.0
11	4.0
12	7.0
13	23.0
14	43.0
15	132.0
16	430.0
17	1116.0
18	2670.0
19	5069.0
20	8947.0
21	14150.0
22	21509.0
23	31456.0
24	44761.0
25	60350.0
26	78252.0
27	97503.0
28	117426.0
29	143699.0
30	177569.0
31	221687.0
32	281328.0
33	402691.0
34	588733.0
35	764910.0
36	432368.0
37	545536.0
38	694938.0
39	830012.0
40	432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.81852225231812	25.127210113516092	31.06254457584984	11.991723058315944
2	27.856244444576156	26.18179996001981	32.46136639943158	13.500589195972454
3	28.35513475175012	26.492948596714978	31.065346422266632	14.086570229268267
4	22.593407003933542	38.14913258606653	26.396895554170197	12.860564855829734
5	23.84676630104367	32.22597537931343	29.12097277233902	14.80628554730388
6	21.721368227815976	40.976249502268146	26.268513514508623	11.033868755407248
7	90.09960384406143	4.123653385016624	4.064599083616616	1.7121436873053189
8	92.40008139723052	2.3720144395669567	3.3570789817156172	1.8708251814869077
9	88.71594072801669	4.092958798309625	4.8265217034435945	2.3645787702300947
10	56.787212516638206	22.575338686656043	10.050672110869781	10.586776685835966
11	47.908107340173075	20.34797854863278	17.717080684376132	14.026833426818017
12	44.19276918874153	20.801895628706497	20.067183248119484	14.938151934432483
13	24.295187451607532	36.210057299555274	21.44337477371947	18.051380475117718
14	16.994473717153962	37.72908911074963	26.211668361244932	19.06476881085148
15	16.663011693937076	23.136695800481235	43.470701038137975	16.72959146744371
16	20.713044768297177	18.38566230530535	40.91613552818487	19.985157398212603
17	20.679709980158975	20.14856251808403	26.11956664056998	33.052160861187005
18	24.639850481980446	24.973324087240158	28.66393055587735	21.72289487490205
19	33.07831142774371	25.199483382626077	21.21717955722565	20.505025632404575
20	34.1784851601821	25.25729840580327	21.742238391510263	18.82197804250437
21	26.12109328765605	28.30100164213345	25.201692530762386	20.37621253944811
22	24.990727864021363	25.577121990058117	23.94662698102665	25.48552316489387
23	24.015272218237975	29.18841465243544	23.86318224735742	22.933130881969166
24	23.435990471566917	23.659509565521628	34.08149816883172	18.823001794079737
25	21.78925912176124	24.647232269655447	30.873653429918008	22.689855178665304
26	19.197982239526617	33.977650245871004	24.11849152181031	22.70587599279207
27	20.22279348757498	32.32118427582238	27.093675244806843	20.3623469917958
28	17.86556058391198	28.714166225286053	33.55755288889224	19.86272030190973
29	19.17515437545137	26.288413808289405	32.72118377292687	21.81524804333235
30	23.204101759469747	28.162274323394502	27.45231158615579	21.181312330979964
31	29.963771766619846	26.374319137850303	22.663902178202104	20.99800691732775
32	33.21795473473429	25.254891691573466	22.756165543862636	18.770988029829606
33	30.05856757038786	26.02389076965823	22.31471308823284	21.602828571721073
34	21.004346992873433	25.897161100960403	27.713817251722734	25.384674654443433
35	22.23942244605862	24.91807742327835	28.677239326357096	24.165260804305934
36	29.952330893751288	25.881589300682485	23.13267263639559	21.033407169170637
37	22.694183672168165	30.975022796433105	26.107910241053983	20.222883290344747
38	23.300945066388493	30.94172392940281	22.700972761562692	23.056358242646006
39	23.05508304331529	29.332584019022384	24.571546495294243	23.040786442368084
40	26.287731307239167	23.217518293273216	24.87827683571679	25.616473563770832
41	19.555469105422524	22.77694590478715	26.87130562630517	30.79627936348515
42	22.525552031096186	24.488281367164554	24.165458370399424	28.820708231339836
43	21.87017141732299	25.674899245782456	25.821834537678278	26.633094799216273
44	21.758438811176564	28.514768155291257	26.209297568123034	23.51749546540914
45	19.559689835601663	32.466610881186085	21.823940951445977	26.149758331766275
46	21.8925861886573	30.593163458821927	23.907383170637655	23.606867181883118
47	21.58863973409759	26.16200742956275	27.25664931138338	24.99270352495628
48	22.895719048083457	23.085274734511582	30.042528795707142	23.976477421697822
49	22.12914464478245	23.471390723409804	29.953085237017348	24.446379394790398
50	19.70071610524669	30.71274482704615	25.59889018145009	23.98764888625707
51	17.992721305904695	32.16877101497066	24.01572123208682	25.822786447037828
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	911.0
1	1722.0
2	2533.0
3	7682.0
4	12831.0
5	9397.0
6	5963.0
7	6572.5
8	7182.0
9	7756.0
10	8330.0
11	8613.0
12	8896.0
13	8697.0
14	8498.0
15	8503.5
16	8509.0
17	9543.5
18	10578.0
19	10423.5
20	10269.0
21	11058.0
22	11847.0
23	14252.0
24	16657.0
25	18406.5
26	27608.5
27	35061.0
28	41386.0
29	47711.0
30	61398.5
31	75086.0
32	86454.5
33	97823.0
34	113803.0
35	129783.0
36	135617.5
37	141452.0
38	179429.0
39	217406.0
40	293876.5
41	370347.0
42	435744.0
43	501141.0
44	495376.5
45	489612.0
46	458908.0
47	428204.0
48	416558.0
49	404912.0
50	394029.0
51	383146.0
52	363536.0
53	343926.0
54	319096.0
55	294266.0
56	298701.5
57	303137.0
58	300309.0
59	297481.0
60	287014.0
61	276547.0
62	236911.0
63	197275.0
64	167362.0
65	137449.0
66	117396.0
67	97343.0
68	80864.5
69	64386.0
70	53688.0
71	42990.0
72	35548.0
73	28106.0
74	21716.0
75	11461.0
76	7596.0
77	5482.0
78	3368.0
79	2817.5
80	2267.0
81	1436.5
82	606.0
83	460.0
84	314.0
85	355.5
86	397.0
87	243.0
88	89.0
89	56.5
90	24.0
91	17.5
92	11.0
93	10.0
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5567757.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.456832519730492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56798161847969	11.069304866321636
2	10.073809945698411	2.912707664411145
3	3.6503558292599347	1.5831774858309837
4	1.8956815674787308	1.0962220372712077
5	1.083644073639279	0.7833030441800776
6	0.7307413168763381	0.6338522899997117
7	0.5122018470532663	0.5183371423401978
8	0.403285517648703	0.4664184949024092
9	0.29507486625468704	0.38392631300032964
>10	2.2158080995955527	6.9184710686664905
>50	0.6994026659186438	7.421623442152571
>100	1.8135354954216438	60.08415437022332
>500	0.053592753797330375	4.830517255997733
>1k	0.0046130471623018554	1.0683353752595628
>5k	2.713557154295209E-4	0.22964914944256895
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6118	0.10988266908918617	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5590	0.10039949660159378	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06453227035590814	0.0
2	0.0	0.0	0.0	0.22114830083281292	0.0
3	0.0	0.0	0.0	0.31522568244267846	0.0
4	0.0	0.0	0.0	0.5040090650507916	0.0
5	0.0	0.0	0.0	0.8543655910270509	0.0
6	0.0	0.0	0.0	1.2059613952261208	0.0
7	0.0	0.0	0.0	1.3992708374305847	0.0
8	0.0	0.0	0.0	1.8617910228481596	0.0
9	0.0	0.0	0.0	1.996387414177738	0.0
10	0.0	0.0	0.0	2.2305391560730827	0.0
11	0.0	0.0	0.0	2.6044958499446005	0.0
12	0.0	0.0	0.0	2.8864585864648906	0.0
13	0.0	0.0	0.0	2.9973470465754883	0.0
14	0.0	0.0	0.0	3.043038695833888	0.0
15	0.0	0.0	0.0	3.1125460396349913	0.0
16	0.0	0.0	0.0	3.2693416756514337	0.0
17	1.7960553953773485E-5	0.0	0.0	3.453976170296225	0.0
18	1.7960553953773485E-5	0.0	0.0	3.6719813741871277	0.0
19	3.592110790754697E-5	0.0	0.0	3.8158633719108073	0.0
20	5.388166186132046E-5	0.0	0.0	3.965241299144341	0.0
21	5.388166186132046E-5	0.0	0.0	4.166579108966142	0.0
22	5.388166186132046E-5	0.0	0.0	4.376178773606679	0.0
23	7.184221581509394E-5	0.0	0.0	4.595495097936206	0.0
24	8.980276976886743E-5	0.0	0.0	4.758684691160193	0.0
25	8.980276976886743E-5	0.0	0.0	4.898345958704735	0.0
26	8.980276976886743E-5	0.0	0.0	5.028757540963085	0.0
27	8.980276976886743E-5	0.0	0.0	5.172890986442116	0.0
28	1.0776332372264092E-4	0.0	0.0	5.314096861626684	0.0
29	1.0776332372264092E-4	0.0	0.0	5.483393043194953	0.0
30	1.0776332372264092E-4	0.0	0.0	5.694124222734577	0.0
31	1.0776332372264092E-4	0.0	0.0	5.885942938960878	0.0
32	1.0776332372264092E-4	0.0	0.0	6.050264047083951	0.0
33	1.257238776764144E-4	0.0	0.0	6.212214362085127	0.0
34	1.257238776764144E-4	0.0	0.0	6.402398667901634	0.0
35	1.257238776764144E-4	0.0	0.0	6.626959473985664	0.0
36	1.4368443163018788E-4	0.0	0.0	6.822424182664581	0.0
37	1.4368443163018788E-4	0.0	0.0	7.0291860797804215	0.0
38	1.6164498558396138E-4	0.0	0.0	7.284944368082156	0.0
39	1.6164498558396138E-4	0.0	0.0	7.723074121230506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	165	0.0	42.272728	1
TACGGGA	1265	0.0	41.264824	4
CGTAAGG	790	0.0	41.012657	2
TACGTAA	50	1.0822987E-9	40.5	1
ATCGGAT	95	0.0	40.263157	20
GCGAATG	700	0.0	39.857143	1
TGTTACG	435	0.0	39.827587	1
TATACGG	450	0.0	39.500004	2
CGTTAGG	930	0.0	39.19355	2
TAAACGG	575	0.0	39.130436	2
TTGTACG	115	0.0	39.130436	1
CGTTTTT	5280	0.0	39.076702	1
ATAGGGC	2675	0.0	38.859814	4
ATGCACG	70	0.0	38.571426	1
TCGTCCC	2120	0.0	38.525944	38
TCGATTG	260	0.0	38.076927	1
AATACGG	435	0.0	37.75862	2
TTAGGGA	4105	0.0	37.710106	4
CGTTGAT	1170	0.0	37.692307	25
TATGGGC	1415	0.0	37.685513	4
>>END_MODULE