FastQCFastQC Report
Thu 26 May 2016
SRR1545300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545300_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2402970
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33280.13849527875920217No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31700.13192008223157176No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.10953112190331131No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24470.10183231584247826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAG551.8189894E-1245.00000425
GATCGAC351.2126657E-745.010
CGCGATA207.0348254E-445.011
ACAATCG253.892081E-545.017
AATCTCG253.892081E-545.018
CGCGTAA207.0348254E-445.031
CGATCGG207.0348254E-445.02
ACCCACG207.0348254E-445.013
ATCGATC351.2126657E-745.010
CCGAATT302.1664946E-644.99999624
TACGTAG600.044.9999961
TATAGCG950.042.631581
CTATATG850.042.352941
TAATGCG850.042.352941
ACAACGT750.042.00000419
TGTAACG700.041.7857131
TAGATCG603.6379788E-1241.2499961
TCGTTCA1150.041.08695616
CGTTAGG3700.040.1351362
AACGCGT451.9297659E-840.00000413
Produced by FastQC (version 0.11.5)