##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545300_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2402970
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260606249765914	31.0	31.0	33.0	30.0	34.0
2	31.579946482894087	31.0	31.0	34.0	30.0	34.0
3	31.685926166369118	31.0	31.0	34.0	30.0	34.0
4	30.29836826926678	35.0	33.0	37.0	0.0	37.0
5	32.86771412044262	35.0	33.0	37.0	19.0	37.0
6	34.50791686954061	35.0	33.0	37.0	30.0	37.0
7	35.455714802931375	36.0	35.0	37.0	33.0	37.0
8	35.6418802565159	37.0	35.0	37.0	35.0	37.0
9	37.64502802781558	39.0	37.0	39.0	35.0	39.0
10	37.100997931726155	39.0	37.0	39.0	33.0	39.0
11	36.70868383708494	39.0	35.0	39.0	32.0	39.0
12	35.826806410400465	37.0	35.0	39.0	31.0	39.0
13	35.44485574102049	37.0	35.0	39.0	30.0	39.0
14	36.36765544305589	38.0	35.0	40.0	31.0	41.0
15	36.65004973012564	38.0	35.0	40.0	31.0	41.0
16	36.80585899948813	38.0	35.0	40.0	32.0	41.0
17	36.761145166190175	38.0	35.0	40.0	32.0	41.0
18	36.7136897256312	38.0	35.0	40.0	31.0	41.0
19	36.690469710400045	38.0	35.0	40.0	31.0	41.0
20	36.59578271888538	38.0	35.0	40.0	31.0	41.0
21	36.44593440617236	38.0	35.0	40.0	31.0	41.0
22	36.31266724095598	38.0	35.0	40.0	31.0	41.0
23	36.26233078232354	38.0	35.0	40.0	31.0	41.0
24	36.16860010736713	38.0	35.0	40.0	30.0	41.0
25	36.07460892145969	37.0	34.0	40.0	30.0	41.0
26	35.89109227331178	37.0	34.0	40.0	30.0	41.0
27	35.896947943586476	37.0	34.0	40.0	30.0	41.0
28	35.88429859715269	37.0	34.0	40.0	30.0	41.0
29	35.93301331269221	37.0	35.0	40.0	30.0	41.0
30	35.89150551192899	37.0	34.0	40.0	30.0	41.0
31	35.803190634922615	37.0	34.0	40.0	30.0	41.0
32	35.6995409846981	37.0	34.0	40.0	30.0	41.0
33	35.559054836306736	37.0	34.0	40.0	29.0	41.0
34	35.47812956466373	37.0	34.0	40.0	29.0	41.0
35	35.329348681007254	37.0	34.0	40.0	29.0	41.0
36	35.18488453871667	36.0	34.0	40.0	27.0	41.0
37	35.14209082926545	36.0	34.0	40.0	27.0	41.0
38	35.122777229844736	36.0	34.0	40.0	28.0	41.0
39	35.04375127446452	36.0	34.0	40.0	27.0	41.0
40	34.904236424091856	36.0	34.0	40.0	27.0	41.0
41	34.84688656121383	36.0	34.0	40.0	27.0	41.0
42	34.806174858612465	36.0	33.0	40.0	27.0	41.0
43	34.773671747878666	35.0	33.0	40.0	27.0	41.0
44	34.70082065111092	35.0	33.0	40.0	27.0	41.0
45	34.59796002446972	35.0	33.0	40.0	27.0	41.0
46	34.52335651298185	35.0	33.0	40.0	27.0	41.0
47	34.45713804167343	35.0	33.0	39.0	27.0	41.0
48	34.34017153772207	35.0	33.0	39.0	26.0	41.0
49	34.29286216640241	35.0	33.0	39.0	26.0	41.0
50	34.14184113825807	35.0	33.0	39.0	26.0	41.0
51	34.04157396888018	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	4.0
14	21.0
15	45.0
16	135.0
17	430.0
18	933.0
19	1834.0
20	3394.0
21	5398.0
22	8254.0
23	12590.0
24	17540.0
25	24189.0
26	31917.0
27	39133.0
28	47620.0
29	58404.0
30	72421.0
31	90771.0
32	115898.0
33	166259.0
34	245746.0
35	322410.0
36	191221.0
37	245130.0
38	317711.0
39	383332.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.94714457525479	24.97888030229258	30.020308201933442	12.053666920519191
2	28.40264339546478	27.218150871629692	31.252741399185176	13.126464333720357
3	29.86941160314111	25.98409468283	30.083854563311235	14.062639150717654
4	23.118640682155835	38.72507771632605	25.66815232816057	12.488129273357554
5	24.16796714066343	32.6919187505462	28.543968505640937	14.596145603149434
6	23.016766751145457	41.275338435352914	24.836015430904258	10.871879382597369
7	89.51834604676712	4.443293091465978	4.056771412044262	1.9815894497226347
8	91.12489960340746	3.1277544039251426	3.770459056917065	1.9768869357503425
9	87.07320524184655	5.052372688797613	5.095111466227211	2.7793106031286285
10	54.309833248022244	25.60826810155766	9.498828533023715	10.583070117396389
11	47.367923860888816	21.638638851088444	17.42768324198804	13.565754046034698
12	45.78796239653429	20.56480105868987	19.15396363666629	14.493272908109548
13	23.416854975301398	37.32173102452382	20.064919661918378	19.196494338256407
14	15.663283353516691	39.65584256149681	24.338755789710234	20.34211829527626
15	15.80539915188288	24.362393205075385	42.2902075348423	17.54200010819944
16	17.708460779785014	18.74650952779269	43.40216482103397	20.142864871388326
17	17.842836156922477	20.855899158125155	26.02346263165999	35.27780205329238
18	22.835574310124553	25.938775764990822	27.382114633141484	23.843535291743134
19	32.611143709659295	26.347103792390254	20.367253856685686	20.67449864126477
20	35.809227747329345	23.163501833148146	21.697607543997634	19.32966287552487
21	26.338198146460424	28.269100321685247	23.15051790076447	22.24218363108986
22	24.008997199299202	27.055435565154788	21.583998135640478	27.351569099905532
23	24.950082606108275	30.70933053679405	22.046592341976805	22.293994515120872
24	23.470205620544576	23.115602774899397	32.867992525915845	20.546199078640182
25	21.729235071598897	24.898438182748848	29.661335763659135	23.710990981993117
26	21.07633470247236	35.93124341960158	21.992451008543593	20.999970869382473
27	23.22646558217539	32.50935300898472	24.775673437454486	19.48850797138541
28	18.74330515986467	29.27269171067471	33.260090637835674	18.723912491624947
29	19.25533818566191	26.30319979025955	31.800688314877007	22.640773709201532
30	22.14001839390421	29.17314822906653	26.23986150472124	22.44697187230802
31	29.804034174375875	27.291476797463137	21.10421686496294	21.80027216319804
32	32.60714865354124	27.645122494246703	21.62623753105532	18.121491321156736
33	29.603906831962114	28.154741840305952	21.203552270731638	21.037799057000296
34	22.810313903211444	27.036958430608788	25.127030300003746	25.025697366176026
35	23.906540655938276	23.65589249969829	27.61882170813618	24.818745136227253
36	29.367199756967423	26.887518362692838	22.31421948671852	21.43106239362123
37	23.740579366367452	31.581293149727212	25.405602233902215	19.272525250003124
38	23.1444004710837	31.125107679246934	22.299903868962158	23.43058798070721
39	24.166136073275986	30.07977627685739	24.262891338635107	21.491196311231516
40	26.961010749197868	24.86701873098707	24.936432831038257	23.235537688776805
41	18.77826190089764	24.93676575238143	27.30196381977303	28.983008526947902
42	23.274739176935206	26.521263270036666	22.64264639175687	27.561351161271258
43	23.701794029888013	28.1195770234335	23.12883639829045	25.04979254838804
44	22.19415972733742	30.612450425931243	25.44709255629492	21.746297290436416
45	20.181400516860386	33.07111616041815	21.548375551921165	25.1991077708003
46	21.473926016554515	32.88255783467958	22.503027503464462	23.140488645301442
47	23.986941160314114	25.687961148079253	26.072069147762978	24.253028543843662
48	24.50638168599691	21.68741182786302	29.37901846465	24.427188021490075
49	20.712618135057866	25.841479502449054	28.72898954210831	24.716912820384774
50	19.252008972230197	32.122207102044555	24.871097017440917	23.75468690828433
51	19.103734129015344	32.627165549299406	22.89013179523673	25.37896852644852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	835.0
2	1376.0
3	3564.0
4	5752.0
5	4442.0
6	3132.0
7	3318.0
8	3504.0
9	3806.0
10	4108.0
11	3981.5
12	3855.0
13	3867.5
14	3880.0
15	4081.5
16	4283.0
17	3867.0
18	3451.0
19	3415.5
20	3380.0
21	3738.0
22	4096.0
23	4567.5
24	5039.0
25	6864.5
26	12887.0
27	17084.0
28	22672.5
29	28261.0
30	32471.0
31	36681.0
32	38969.0
33	41257.0
34	48627.5
35	55998.0
36	59204.5
37	62411.0
38	76626.0
39	90841.0
40	124065.5
41	157290.0
42	185979.0
43	214668.0
44	211998.5
45	209329.0
46	197682.5
47	186036.0
48	178856.5
49	171677.0
50	161421.0
51	151165.0
52	142373.5
53	133582.0
54	126906.5
55	120231.0
56	122207.5
57	124184.0
58	131841.5
59	139499.0
60	137876.0
61	136253.0
62	117146.0
63	98039.0
64	78552.5
65	59066.0
66	48232.0
67	37398.0
68	32661.5
69	27925.0
70	24140.5
71	20356.0
72	16432.5
73	12509.0
74	9665.0
75	5631.5
76	4442.0
77	3503.5
78	2565.0
79	1890.0
80	1215.0
81	896.0
82	577.0
83	555.0
84	533.0
85	365.5
86	198.0
87	113.5
88	29.0
89	16.0
90	3.0
91	4.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2402970.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.181764097544633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79590447930885	12.750567381368361
2	8.264397156539221	2.6746505039107267
3	2.7721506869300527	1.3457486537624526
4	1.3580145635539878	0.8790028523384265
5	0.7898255457949834	0.6390385330134428
6	0.529587177822059	0.5141792868360587
7	0.39106546802648273	0.4429690405210299
8	0.2721912227418949	0.35236273246652555
9	0.2259845740800443	0.32911461607026393
>10	3.046686955298225	12.671949226831789
>50	1.700895215551991	20.05670401560177
>100	1.8443153546949147	45.8602196453941
>500	0.007016874732234448	0.7721087990094686
>1k	0.0019647249250256456	0.7113847128756063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3328	0.13849527875920217	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3170	0.13192008223157176	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2632	0.10953112190331131	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2447	0.10183231584247826	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047108370058719	0.0
2	0.0	0.0	0.0	0.15002268026650353	0.0
3	0.0	0.0	0.0	0.21261189278268144	0.0
4	0.0	0.0	0.0	0.35518545799573026	0.0
5	4.161516789639488E-5	0.0	0.0	0.6121175045880722	0.0
6	4.161516789639488E-5	0.0	0.0	0.8492823464296266	0.0
7	8.323033579278976E-5	0.0	0.0	0.9888180043862387	0.0
8	8.323033579278976E-5	0.0	0.0	1.3212815807105374	0.0
9	8.323033579278976E-5	0.0	0.0	1.4201592196323716	0.0
10	8.323033579278976E-5	0.0	0.0	1.591572096197622	0.0
11	1.2484550368918463E-4	0.0	0.0	1.8525408140759143	0.0
12	1.2484550368918463E-4	0.0	0.0	2.057453900797763	0.0
13	1.2484550368918463E-4	0.0	0.0	2.1429730708248544	0.0
14	1.2484550368918463E-4	0.0	0.0	2.1772223540035873	0.0
15	1.2484550368918463E-4	0.0	0.0	2.2351090525474726	8.323033579278976E-5
16	1.2484550368918463E-4	0.0	0.0	2.3577073371702517	8.323033579278976E-5
17	1.2484550368918463E-4	0.0	0.0	2.4947877002209764	8.323033579278976E-5
18	1.2484550368918463E-4	0.0	0.0	2.6583769252217047	8.323033579278976E-5
19	1.2484550368918463E-4	0.0	0.0	2.765577597722818	8.323033579278976E-5
20	1.2484550368918463E-4	0.0	0.0	2.867576374236882	8.323033579278976E-5
21	1.6646067158557952E-4	0.0	0.0	3.021136343774579	8.323033579278976E-5
22	1.6646067158557952E-4	0.0	0.0	3.1839348805852756	8.323033579278976E-5
23	1.6646067158557952E-4	0.0	0.0	3.3617148778386747	8.323033579278976E-5
24	1.6646067158557952E-4	0.0	0.0	3.489390212944814	8.323033579278976E-5
25	1.6646067158557952E-4	0.0	0.0	3.6002946353887064	8.323033579278976E-5
26	1.6646067158557952E-4	0.0	0.0	3.708161150576162	8.323033579278976E-5
27	1.6646067158557952E-4	0.0	0.0	3.8060400254684827	8.323033579278976E-5
28	1.6646067158557952E-4	0.0	0.0	3.912117088436393	8.323033579278976E-5
29	1.6646067158557952E-4	0.0	0.0	4.0272246428378216	8.323033579278976E-5
30	1.6646067158557952E-4	0.0	0.0	4.167426143480776	8.323033579278976E-5
31	2.080758394819744E-4	0.0	0.0	4.318322742273104	8.323033579278976E-5
32	2.080758394819744E-4	0.0	0.0	4.450742206519433	8.323033579278976E-5
33	2.080758394819744E-4	0.0	0.0	4.575338019201238	8.323033579278976E-5
34	2.080758394819744E-4	0.0	0.0	4.71337553111358	8.323033579278976E-5
35	2.080758394819744E-4	0.0	0.0	4.887826314935268	8.323033579278976E-5
36	2.4969100737836926E-4	0.0	0.0	5.065814388028149	8.323033579278976E-5
37	2.4969100737836926E-4	0.0	0.0	5.23098498940894	8.323033579278976E-5
38	2.4969100737836926E-4	0.0	0.0	5.3701877260223805	8.323033579278976E-5
39	2.913061752747641E-4	0.0	0.0	5.517963187222479	8.323033579278976E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAG	55	1.8189894E-12	45.000004	25
GATCGAC	35	1.2126657E-7	45.0	10
CGCGATA	20	7.0348254E-4	45.0	11
ACAATCG	25	3.892081E-5	45.0	17
AATCTCG	25	3.892081E-5	45.0	18
CGCGTAA	20	7.0348254E-4	45.0	31
CGATCGG	20	7.0348254E-4	45.0	2
ACCCACG	20	7.0348254E-4	45.0	13
ATCGATC	35	1.2126657E-7	45.0	10
CCGAATT	30	2.1664946E-6	44.999996	24
TACGTAG	60	0.0	44.999996	1
TATAGCG	95	0.0	42.63158	1
CTATATG	85	0.0	42.35294	1
TAATGCG	85	0.0	42.35294	1
ACAACGT	75	0.0	42.000004	19
TGTAACG	70	0.0	41.785713	1
TAGATCG	60	3.6379788E-12	41.249996	1
TCGTTCA	115	0.0	41.086956	16
CGTTAGG	370	0.0	40.135136	2
AACGCGT	45	1.9297659E-8	40.000004	13
>>END_MODULE