FastQCFastQC Report
Thu 26 May 2016
SRR1545299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545299_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3063822
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34150.11146208885503141No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31240.10196414804776517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTC302.166691E-645.00000426
CGAAACG302.166691E-645.00000424
TACCGTA302.166691E-645.00000445
TACCGGA302.166691E-645.00000431
AAATGCG1100.045.0000041
CGGACGT302.166691E-645.00000426
AACGTCC453.8562575E-1045.033
TCGTTAC351.2128112E-745.042
CGTATTA253.8923332E-545.031
GTCGAAT351.2128112E-745.011
GTCGAAC207.03513E-445.014
CGACGGA253.8923332E-545.031
TCCGATC406.8212103E-945.019
CGTGCAA253.8923332E-545.029
ACCGCGA207.03513E-445.037
TATATCG351.2128112E-745.01
ACCTATC207.03513E-445.024
GCCGAAT207.03513E-445.014
GTACTCG351.2128112E-745.033
CATACGT351.2128112E-745.010