##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545299_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3063822
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271995566322065	31.0	31.0	33.0	30.0	34.0
2	31.580324836103404	31.0	31.0	34.0	30.0	34.0
3	31.668750338629334	31.0	31.0	34.0	30.0	34.0
4	30.283164948877577	35.0	33.0	37.0	0.0	37.0
5	32.83864467322188	35.0	33.0	37.0	19.0	37.0
6	34.46285391253147	35.0	33.0	37.0	30.0	37.0
7	35.48355811793244	36.0	35.0	37.0	33.0	37.0
8	35.671808936681046	37.0	35.0	37.0	35.0	37.0
9	37.69690341018506	39.0	37.0	39.0	35.0	39.0
10	37.159503391515564	39.0	37.0	39.0	33.0	39.0
11	36.68119590498404	39.0	35.0	39.0	32.0	39.0
12	35.39909694492696	35.0	35.0	39.0	31.0	39.0
13	34.86327632610511	35.0	34.0	39.0	30.0	39.0
14	35.64093867071912	36.0	34.0	40.0	29.0	41.0
15	36.04015344233444	36.0	35.0	40.0	31.0	41.0
16	36.25736416802282	36.0	35.0	40.0	32.0	41.0
17	36.211649371275485	36.0	35.0	40.0	31.0	41.0
18	36.2002188769452	36.0	35.0	40.0	31.0	41.0
19	36.18049449347906	36.0	35.0	40.0	31.0	41.0
20	36.03557811126103	36.0	34.0	40.0	31.0	41.0
21	35.83059981944121	36.0	34.0	40.0	30.0	41.0
22	35.69525285737879	35.0	34.0	40.0	30.0	41.0
23	35.69313948395175	35.0	34.0	40.0	30.0	41.0
24	35.6213507181553	35.0	34.0	40.0	30.0	41.0
25	35.4945479861428	35.0	34.0	40.0	30.0	41.0
26	35.32556591081336	35.0	34.0	40.0	30.0	41.0
27	35.31272280178157	35.0	34.0	40.0	29.0	41.0
28	35.39162131481529	36.0	34.0	40.0	30.0	41.0
29	35.47645457209982	36.0	34.0	40.0	30.0	41.0
30	35.451759926000925	36.0	34.0	40.0	30.0	41.0
31	35.30225319878244	35.0	34.0	40.0	30.0	41.0
32	35.17172733925143	35.0	34.0	40.0	29.0	41.0
33	35.01298313022101	35.0	34.0	40.0	29.0	41.0
34	34.9633918027875	35.0	34.0	40.0	29.0	41.0
35	34.85988415776112	35.0	34.0	40.0	29.0	41.0
36	34.64392383108418	35.0	33.0	40.0	27.0	41.0
37	34.58323329488462	35.0	33.0	40.0	27.0	41.0
38	34.614557242555215	35.0	33.0	40.0	27.0	41.0
39	34.57248528145565	35.0	33.0	40.0	27.0	41.0
40	34.432550259120795	35.0	33.0	39.0	26.0	41.0
41	34.43640263696781	35.0	33.0	39.0	27.0	41.0
42	34.3672324958826	35.0	33.0	39.0	27.0	41.0
43	34.29435489398536	35.0	33.0	39.0	27.0	41.0
44	34.2595842708878	35.0	33.0	39.0	27.0	41.0
45	34.14735353424579	35.0	33.0	39.0	26.0	41.0
46	34.10116710435528	35.0	33.0	39.0	26.0	41.0
47	34.041718154644755	35.0	33.0	39.0	26.0	41.0
48	33.98676718164437	35.0	33.0	39.0	26.0	41.0
49	33.9817277243913	35.0	33.0	38.0	26.0	40.0
50	33.78362417921146	35.0	33.0	38.0	26.0	40.0
51	33.71921932801579	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	3.0
13	8.0
14	20.0
15	57.0
16	212.0
17	592.0
18	1338.0
19	2691.0
20	4868.0
21	8003.0
22	12397.0
23	17925.0
24	25055.0
25	34430.0
26	44667.0
27	55143.0
28	66206.0
29	80231.0
30	99040.0
31	124923.0
32	160694.0
33	237885.0
34	366478.0
35	508630.0
36	211679.0
37	256289.0
38	332478.0
39	411564.0
40	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.20206656914142	24.954582870675907	31.0322858181709	11.811064742011775
2	27.714110023362977	27.054737514124515	31.986518799068612	13.244633663443894
3	29.05720371483722	25.910447800165937	31.282528815316297	13.74981966968055
4	22.892648463259288	39.089411852255125	25.446517454342978	12.571422230142613
5	24.389504351101337	32.974174087136916	28.772591880337693	13.86372968142405
6	22.076413055327627	41.56494731090775	25.62870819518889	10.72993143857574
7	89.24105251545292	4.820939336554146	4.115154209350282	1.8228539386426497
8	91.78379161713703	2.8094647796118704	3.5455062337172327	1.8612373695338698
9	88.5528597940742	4.4042049440209	4.635843727213917	2.407091534690984
10	62.74705253764742	19.906737401846453	8.465929156458827	8.880280904047298
11	56.12281000658654	17.74607010459485	14.674677575916617	11.45644231290199
12	50.66776072500295	20.713931814576693	16.295333083971588	12.322974376448762
13	21.410741224522834	46.12046652840798	17.929142097680607	14.539650149388574
14	13.314905369828924	47.48076095804522	23.855628688611805	15.348704983514056
15	13.43694248556215	23.13434004978096	51.11759103498833	12.311126429668565
16	15.040658367228907	16.49573637110772	49.73728891560933	18.726316346054045
17	16.26514203501378	17.471282600621056	25.802935026904304	40.46064033746086
18	22.456493882477506	24.480338609749523	31.462891773738814	21.600275734034156
19	34.83985035684188	24.36864804809157	20.59865096601565	20.192850629050906
20	38.1353094272448	23.350997544896536	20.076982278996624	18.436710748862044
21	24.741026077885724	30.06653128021145	23.174551263095573	22.017891378807256
22	23.639069110411768	26.303159909420327	21.235208833933562	28.822562146234343
23	23.02375921316578	32.967678931739506	21.028538864202947	22.98002299089177
24	22.346304713524482	23.348484344064374	37.35034215434187	16.95486878806928
25	18.45260592815118	25.08060194097438	34.933458928097	21.533333202777445
26	17.471837463142442	39.72228151635441	22.573667791405637	20.23221322909751
27	19.19794948923273	37.203793170752085	25.913940170153488	17.684317169861696
28	15.524792236624712	29.076101679536215	39.12482513670834	16.27428094713074
29	16.110890254068284	24.94025436203539	37.567685067866215	21.38117031603011
30	20.272652915215048	32.07399124361663	27.76146917151192	19.891886669656397
31	33.07708476536823	26.520470183972826	19.661063860759533	20.741381189899414
32	36.320419397732636	26.670935844184157	21.184781622431068	15.823863135652136
33	30.831164473654148	28.18943137036029	21.014014521731355	19.965389634254212
34	19.82840386941539	27.402734231949506	26.133502533763387	26.635359364871718
35	21.76134906009553	24.13603009574316	29.85013489687064	24.252485947290673
36	33.563144334102965	23.799718129839135	23.349789902938227	19.28734763311968
37	21.940537015531582	33.90660423484132	26.320621759358083	17.832236990269017
38	21.18794760270016	35.02370568525195	21.251234569110085	22.537112142937808
39	22.456428604533816	32.072718323714625	24.733943420995082	20.736909650756473
40	26.90006142654501	24.771053931984298	22.919347142229544	25.409537499241143
41	16.893050575392436	23.596834280842685	25.7893898535881	33.72072529017678
42	22.506300953514923	25.457516787855166	21.74711846837055	30.28906379025936
43	22.45427443239196	26.992854023503977	23.590175930586046	26.96269561351802
44	21.001415878598692	31.643515843936104	26.38031843886492	20.974749838600285
45	18.295710390486132	37.832582963370584	19.795112118132188	24.076594528011093
46	21.284395764505902	35.23585900225274	21.74065595194499	21.739089281296366
47	21.85326040481464	25.941585379307284	27.09674387089067	25.1084103449874
48	24.44655727388863	20.290016848237265	31.595797667096846	23.66762821077726
49	19.831765683515556	23.088906600970944	32.2731542498226	24.806173465690893
50	18.133723173213063	35.31944740915105	24.786067859033587	21.760761558602297
51	17.741761760311142	35.705207417402185	21.449451045132516	25.103579777154156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	945.0
2	1447.0
3	3888.5
4	6330.0
5	4928.5
6	3527.0
7	3891.5
8	4256.0
9	4726.0
10	5196.0
11	5272.5
12	5349.0
13	5465.5
14	5582.0
15	5470.0
16	5358.0
17	5166.5
18	4975.0
19	4611.5
20	4248.0
21	4447.0
22	4646.0
23	6087.0
24	7528.0
25	9460.5
26	14942.0
27	18491.0
28	23748.5
29	29006.0
30	35134.5
31	41263.0
32	45136.0
33	49009.0
34	55257.5
35	61506.0
36	64064.5
37	66623.0
38	95154.5
39	123686.0
40	189472.5
41	255259.0
42	304505.0
43	353751.0
44	341174.5
45	328598.0
46	297941.5
47	267285.0
48	247852.0
49	228419.0
50	221635.0
51	214851.0
52	194907.0
53	174963.0
54	159320.5
55	143678.0
56	135685.0
57	127692.0
58	129536.5
59	131381.0
60	126805.5
61	122230.0
62	108157.5
63	94085.0
64	78295.0
65	62505.0
66	48435.5
67	34366.0
68	28880.5
69	23395.0
70	20102.5
71	16810.0
72	14427.5
73	12045.0
74	9485.5
75	5092.5
76	3259.0
77	2386.0
78	1513.0
79	1045.0
80	577.0
81	339.5
82	102.0
83	149.0
84	196.0
85	124.5
86	53.0
87	35.5
88	18.0
89	9.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3063822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.019422543244223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70813642384307	10.894231995739231
2	9.833371077060875	2.757163683076659
3	3.5192505700082712	1.4801358232949713
4	1.770253086924171	0.992717041362896
5	1.0148582850492365	0.711386355980872
6	0.6245783705416833	0.5253736852796891
7	0.42868660694047167	0.42069570769296866
8	0.3152009965167249	0.35351487649756935
9	0.22284092855302007	0.28116910235823167
>10	1.7889623009194766	5.714461808771009
>50	0.7310587108092931	7.5744814922685295
>100	1.9791229958874061	61.73566997773228
>500	0.057109524642414713	5.04773248322635
>1k	0.006570122303994613	1.5112659667187396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3415	0.11146208885503141	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3124	0.10196414804776517	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0633196053817748	0.0
2	0.0	0.0	0.0	0.1993262010652055	0.0
3	3.2638971846275664E-5	0.0	0.0	0.2863417000073764	0.0
4	3.2638971846275664E-5	0.0	0.0	0.48948666077859615	0.0
5	3.2638971846275664E-5	0.0	0.0	0.8058562148845462	0.0
6	3.2638971846275664E-5	0.0	0.0	1.1293410648529842	0.0
7	3.2638971846275664E-5	0.0	0.0	1.307615129077342	0.0
8	6.527794369255133E-5	0.0	0.0	1.745956520972824	0.0
9	6.527794369255133E-5	0.0	0.0	1.8635547365349554	0.0
10	6.527794369255133E-5	0.0	0.0	2.049498959143188	0.0
11	6.527794369255133E-5	0.0	0.0	2.360515721866349	0.0
12	6.527794369255133E-5	0.0	0.0	2.6049816209949532	0.0
13	9.791691553882699E-5	0.0	0.0	2.705248542506712	0.0
14	9.791691553882699E-5	0.0	0.0	2.7451986440465537	0.0
15	9.791691553882699E-5	0.0	0.0	2.806788383920476	0.0
16	9.791691553882699E-5	0.0	0.0	2.9510852784528607	0.0
17	9.791691553882699E-5	0.0	0.0	3.1071322028499044	0.0
18	9.791691553882699E-5	0.0	0.0	3.307013266436497	0.0
19	9.791691553882699E-5	0.0	0.0	3.4262107916190954	0.0
20	1.3055588738510265E-4	0.0	0.0	3.5392069121509016	0.0
21	1.3055588738510265E-4	0.0	0.0	3.7096149841603068	0.0
22	1.3055588738510265E-4	0.0	0.0	3.8766286030976995	0.0
23	1.3055588738510265E-4	0.0	0.0	4.0663589464401	0.0
24	1.3055588738510265E-4	0.0	0.0	4.203638462025536	0.0
25	1.3055588738510265E-4	0.0	0.0	4.316406109754418	0.0
26	1.3055588738510265E-4	0.0	0.0	4.417978590140028	0.0
27	1.3055588738510265E-4	0.0	0.0	4.51602606156624	0.0
28	1.6319485923137832E-4	0.0	0.0	4.62618259154742	0.0
29	1.6319485923137832E-4	0.0	0.0	4.73940718488215	0.0
30	1.6319485923137832E-4	0.0	0.0	4.896531195350121	0.0
31	1.9583383107765398E-4	0.0	0.0	5.0531329822685525	0.0
32	1.9583383107765398E-4	0.0	0.0	5.192142363361841	0.0
33	1.9583383107765398E-4	0.0	0.0	5.324134365508179	0.0
34	1.9583383107765398E-4	0.0	0.0	5.464122915756855	0.0
35	1.9583383107765398E-4	0.0	0.0	5.659010216650968	0.0
36	1.9583383107765398E-4	0.0	0.0	5.818973817669565	0.0
37	1.9583383107765398E-4	0.0	0.0	5.983604791662179	0.0
38	2.611117747702053E-4	0.0	0.0	6.1144217908220515	0.0
39	2.93750746616481E-4	0.0	0.0	6.261003413383675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTC	30	2.166691E-6	45.000004	26
CGAAACG	30	2.166691E-6	45.000004	24
TACCGTA	30	2.166691E-6	45.000004	45
TACCGGA	30	2.166691E-6	45.000004	31
AAATGCG	110	0.0	45.000004	1
CGGACGT	30	2.166691E-6	45.000004	26
AACGTCC	45	3.8562575E-10	45.0	33
TCGTTAC	35	1.2128112E-7	45.0	42
CGTATTA	25	3.8923332E-5	45.0	31
GTCGAAT	35	1.2128112E-7	45.0	11
GTCGAAC	20	7.03513E-4	45.0	14
CGACGGA	25	3.8923332E-5	45.0	31
TCCGATC	40	6.8212103E-9	45.0	19
CGTGCAA	25	3.8923332E-5	45.0	29
ACCGCGA	20	7.03513E-4	45.0	37
TATATCG	35	1.2128112E-7	45.0	1
ACCTATC	20	7.03513E-4	45.0	24
GCCGAAT	20	7.03513E-4	45.0	14
GTACTCG	35	1.2128112E-7	45.0	33
CATACGT	35	1.2128112E-7	45.0	10
>>END_MODULE