FastQCFastQC Report
Thu 26 May 2016
SRR1545298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545298_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2155034
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29440.13661037366463824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22750.10556677992087364No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22070.10241137726829369No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCC302.166389E-645.00000418
CCGTGAT302.166389E-645.00000430
ATTAACG600.045.0000041
CATACGT302.166389E-645.00000437
CTTTCGT453.8562575E-1045.00000422
ACGTAAG1350.045.0000041
CGACTTA302.166389E-645.00000432
TAGTCGA453.8562575E-1045.00000421
TCGTTAC253.891947E-545.026
ACGCCTA406.8193913E-945.01
CGTATCG406.8193913E-945.040
CGAGTCA253.891947E-545.045
GCGAATC207.034663E-445.012
GACGTAA253.891947E-545.027
ATTACGT253.891947E-545.012
TAAACCG253.891947E-545.032
GTAGGTT253.891947E-545.032
CGCGATA253.891947E-545.011
TCTAGAT253.891947E-545.044
TACGATG207.034663E-445.016