##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545298_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2155034
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290455278199786	31.0	31.0	33.0	30.0	34.0
2	31.61606731030694	31.0	31.0	34.0	30.0	34.0
3	31.714607286938396	31.0	31.0	34.0	30.0	34.0
4	30.31161318104494	35.0	33.0	37.0	0.0	37.0
5	32.871547734281684	35.0	33.0	37.0	19.0	37.0
6	34.5103594653263	35.0	33.0	37.0	30.0	37.0
7	35.4844239116413	36.0	35.0	37.0	33.0	37.0
8	35.67988904119378	37.0	35.0	37.0	35.0	37.0
9	37.72182387841677	39.0	37.0	39.0	35.0	39.0
10	37.17002562372566	39.0	37.0	39.0	33.0	39.0
11	36.72014548262348	39.0	35.0	39.0	32.0	39.0
12	35.469843631237374	37.0	35.0	39.0	31.0	39.0
13	34.95037340478155	36.0	34.0	39.0	30.0	39.0
14	35.763078911980045	37.0	34.0	40.0	30.0	41.0
15	36.117894659666625	37.0	35.0	40.0	31.0	41.0
16	36.34602934338855	37.0	35.0	40.0	32.0	41.0
17	36.29676329932614	37.0	35.0	40.0	31.0	41.0
18	36.26434988960731	36.0	35.0	40.0	31.0	41.0
19	36.26341208537777	37.0	35.0	40.0	31.0	41.0
20	36.12237022710546	36.0	35.0	40.0	31.0	41.0
21	35.93616202807009	36.0	34.0	40.0	31.0	41.0
22	35.81198672503543	36.0	34.0	40.0	30.0	41.0
23	35.77107043322751	36.0	34.0	40.0	30.0	41.0
24	35.693660517653086	35.0	34.0	40.0	30.0	41.0
25	35.54417656519572	35.0	34.0	40.0	30.0	41.0
26	35.37861258801485	35.0	34.0	40.0	30.0	41.0
27	35.35741477860674	35.0	34.0	40.0	29.0	41.0
28	35.398172836252236	36.0	34.0	40.0	29.0	41.0
29	35.49452352027857	36.0	34.0	40.0	30.0	41.0
30	35.44361620280701	36.0	34.0	40.0	30.0	41.0
31	35.333258315182036	35.0	34.0	40.0	30.0	41.0
32	35.18908425574724	35.0	34.0	40.0	29.0	41.0
33	35.0478841633125	35.0	34.0	40.0	29.0	41.0
34	35.0108118943831	35.0	34.0	40.0	29.0	41.0
35	34.8926216477327	35.0	34.0	40.0	28.0	41.0
36	34.696414534527065	35.0	33.0	40.0	27.0	41.0
37	34.638227517524086	35.0	33.0	40.0	27.0	41.0
38	34.63289024674321	35.0	33.0	40.0	27.0	41.0
39	34.61637681818477	35.0	33.0	40.0	27.0	41.0
40	34.4898164019686	35.0	33.0	40.0	27.0	41.0
41	34.48644986575618	35.0	33.0	39.0	27.0	41.0
42	34.43606736599051	35.0	33.0	39.0	27.0	41.0
43	34.35961427986751	35.0	33.0	39.0	27.0	41.0
44	34.298406428854484	35.0	33.0	39.0	27.0	41.0
45	34.214588725746324	35.0	33.0	39.0	27.0	41.0
46	34.16517929647514	35.0	33.0	39.0	27.0	41.0
47	34.09005426364503	35.0	33.0	39.0	26.0	41.0
48	34.02016302294999	35.0	33.0	39.0	26.0	41.0
49	33.99032776281024	35.0	33.0	39.0	26.0	40.0
50	33.81627575249393	35.0	33.0	38.0	26.0	40.0
51	33.73527842252141	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	4.0
13	2.0
14	11.0
15	41.0
16	135.0
17	385.0
18	921.0
19	1894.0
20	3402.0
21	5300.0
22	8226.0
23	12330.0
24	17617.0
25	23938.0
26	31044.0
27	37997.0
28	45824.0
29	56031.0
30	68638.0
31	86790.0
32	111417.0
33	165474.0
34	254172.0
35	350456.0
36	154017.0
37	187062.0
38	239801.0
39	291859.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.918289920251837	24.873528677505785	30.816079931917546	12.39210147032483
2	27.551212649081176	26.75776809089787	31.815878542983544	13.875140717037409
3	28.858152585991682	26.432761617682132	30.617289564805013	14.091796231521172
4	22.910821824620864	38.548394132064736	26.025668272519138	12.515115770795262
5	23.830575294867735	32.345104531993464	29.503942861226317	14.32037731191248
6	22.11403625186424	41.783702716523266	25.076541715815154	11.025719315797337
7	90.31398112512377	4.212972973976281	3.881191665653535	1.5918542352464045
8	92.37956338507884	2.6141582917021267	3.277813714307988	1.7284646089110427
9	89.06657621179063	4.185502409706761	4.393573372856299	2.3543480056463144
10	61.90338528301642	20.5485853123431	8.139546754250745	9.40848265038974
11	55.06785507792452	18.49901208055186	14.218105143584742	12.215027697938872
12	49.39379146686317	20.50116146659403	17.122374867403483	12.982672199139317
13	22.004200397766347	43.8456655440239	18.611260889619373	15.538873168590381
14	14.682320557355474	45.33529401392275	23.478887108045626	16.503498320676147
15	14.520652574390938	22.78052225626139	48.761179638001074	13.937645531346604
16	16.22229626075505	16.908967561532673	47.64699768077905	19.221738496933227
17	17.361211006415676	18.838125059743838	25.009489409447834	38.79117452439265
18	22.90223727328664	25.372175102573784	30.20096202658519	21.52462559755438
19	34.102710212460686	25.321549451191956	19.972306701425595	20.60343363492177
20	37.227858121960026	23.727792693757966	20.481347394055035	18.563001790226977
21	25.270227755107342	29.175456164496712	23.401765354978156	22.15255072541779
22	24.84647574005793	25.64785520785287	21.354837093057462	28.150831959031734
23	24.182217078709662	31.194449832346034	20.866352920649973	23.756980168294326
24	23.324411587009763	22.612033035209652	35.32463988967228	18.738915488108308
25	19.676209284865113	25.09849032544266	33.0911252444277	22.13417514526453
26	18.852509983601188	37.859866712079715	22.330227736546153	20.957395567772945
27	20.736146158250868	35.13318119342897	26.096107996439965	18.034564651880203
28	16.022949057880293	29.176801850922075	37.88710526144831	16.913143829749323
29	17.460466981031388	24.982761292861273	35.72741775767807	21.82935396842927
30	20.99419313105965	31.900424772880616	26.876838138052577	20.228543958007158
31	32.9268586945728	26.86616545261003	19.751150097863885	20.455825754953285
32	34.95805634621078	26.982915350755487	21.043426693035933	17.0156016099978
33	29.975211527985174	30.04110375984787	20.499398153347002	19.484286558819957
34	21.017533830092706	28.103454516262854	24.841742636079058	26.037269017565386
35	22.398486520398286	24.04667397358928	28.734488643798663	24.820350862213775
36	32.514521812648894	25.420062978124708	22.940426925978894	19.124988283247504
37	22.843630309312985	32.969874257204296	25.69657833704712	18.4899170964356
38	21.8354327588335	33.48230236738725	20.98700066913097	23.69526420464828
39	22.882980036509863	32.31730914686265	24.34249297226865	20.457217844358837
40	26.44092854219469	25.07352552210313	23.19155985474011	25.293986080962068
41	17.3308170543945	23.57099702371285	26.085806534838895	33.012379387053755
42	23.459351453387743	25.085126267149384	21.9505121496923	29.505010129770575
43	23.30459751447077	26.712525185217494	23.89261607937508	26.090261220936657
44	20.928718526018613	30.755848863637418	25.913929896233658	22.40150271411031
45	18.792789348103092	36.953662912046866	19.760801917742366	24.49274582210768
46	22.291806068952972	34.430918491309185	22.239973940086326	21.037301499651516
47	22.82799250499064	25.42052700792656	26.458190450823516	25.293290036259286
48	24.690236905775038	20.599860605447525	30.20852571235535	24.50137677642209
49	20.81684094079258	22.907852033889025	31.575557508605435	24.699749516712963
50	18.979190119506235	32.97553542078687	25.016913886277433	23.028360573429467
51	18.0020825657507	35.14269380436689	21.34003454237845	25.515189087503952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	590.0
2	978.0
3	2855.0
4	4732.0
5	3632.5
6	2533.0
7	2766.5
8	3000.0
9	3225.0
10	3450.0
11	3500.0
12	3550.0
13	3527.0
14	3504.0
15	3541.5
16	3579.0
17	3530.0
18	3481.0
19	3228.5
20	2976.0
21	3158.0
22	3340.0
23	3786.5
24	4233.0
25	5207.0
26	8417.5
27	10654.0
28	15409.5
29	20165.0
30	23515.0
31	26865.0
32	29930.5
33	32996.0
34	36994.0
35	40992.0
36	42479.5
37	43967.0
38	63443.5
39	82920.0
40	122991.5
41	163063.0
42	193856.0
43	224649.0
44	229200.5
45	233752.0
46	211048.0
47	188344.0
48	176186.0
49	164028.0
50	147456.0
51	130884.0
52	127129.0
53	123374.0
54	114476.0
55	105578.0
56	105420.0
57	105262.0
58	104666.5
59	104071.0
60	101647.0
61	99223.0
62	87106.5
63	74990.0
64	61023.0
65	47056.0
66	39983.0
67	32910.0
68	26513.5
69	20117.0
70	17397.0
71	14677.0
72	11703.5
73	8730.0
74	6642.0
75	3831.5
76	3109.0
77	2244.0
78	1379.0
79	1059.0
80	739.0
81	444.5
82	150.0
83	102.0
84	54.0
85	40.5
86	27.0
87	18.5
88	10.0
89	7.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2155034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.541951565311688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76461386235832	12.241558137092504
2	9.017334122647437	2.8029394036483763
3	3.1014352720256233	1.4460707034231453
4	1.4449965137515413	0.8983226331508279
5	0.763841641307475	0.5935794896384479
6	0.4841206750274758	0.45145080498258167
7	0.34463221493370866	0.3749380034642051
8	0.2553288587530032	0.31746470047723874
9	0.19689740124919666	0.275415288619568
>10	2.3478811381020375	9.081601646480882
>50	1.2636515042876673	14.357202460168278
>100	1.9980144711882413	54.946535250130935
>500	0.014973715489517037	1.5370700342141808
>1k	0.002278608878839549	0.675851444508829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2944	0.13661037366463824	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2275	0.10556677992087364	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2207	0.10241137726829369	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04955838283757936	0.0
2	0.0	0.0	0.0	0.17507844423800273	0.0
3	0.0	0.0	0.0	0.2486271678312268	0.0
4	0.0	0.0	0.0	0.42305597034663955	0.0
5	0.0	0.0	0.0	0.7210095061145207	0.0
6	0.0	0.0	0.0	1.0025363868969122	0.0
7	0.0	0.0	0.0	1.1654108473462599	0.0
8	0.0	0.0	0.0	1.5626667607100397	0.0
9	0.0	0.0	0.0	1.6742659280549634	0.0
10	0.0	0.0	0.0	1.8749588173550857	0.0
11	0.0	0.0	0.0	2.171195442856122	0.0
12	0.0	0.0	0.0	2.4157391484310686	0.0
13	4.6402980184999404E-5	0.0	0.0	2.5161551975514076	0.0
14	9.280596036999881E-5	0.0	0.0	2.548126850898872	0.0
15	9.280596036999881E-5	0.0	0.0	2.6068730238130815	0.0
16	9.280596036999881E-5	0.0	0.0	2.7415808752901345	0.0
17	1.8561192073999761E-4	0.0	0.0	2.8982837393748775	0.0
18	1.8561192073999761E-4	0.0	0.0	3.0899280475389252	0.0
19	1.8561192073999761E-4	0.0	0.0	3.208023632109749	0.0
20	1.8561192073999761E-4	0.0	0.0	3.3225925901865123	0.0
21	1.8561192073999761E-4	0.0	0.0	3.4917314529608348	0.0
22	1.8561192073999761E-4	0.0	0.0	3.6651393899121776	0.0
23	1.8561192073999761E-4	0.0	0.0	3.8550667878093803	0.0
24	1.8561192073999761E-4	0.0	0.0	3.9942757283643786	0.0
25	1.8561192073999761E-4	0.0	0.0	4.114598655984082	0.0
26	1.8561192073999761E-4	0.0	0.0	4.2235064504782756	0.0
27	1.8561192073999761E-4	0.0	0.0	4.324247320459909	0.0
28	1.8561192073999761E-4	0.0	0.0	4.435289652042613	0.0
29	1.8561192073999761E-4	0.0	0.0	4.547770476011052	0.0
30	1.8561192073999761E-4	0.0	0.0	4.700482683799884	0.0
31	2.3201490092499702E-4	0.0	0.0	4.866419740941442	0.0
32	2.3201490092499702E-4	0.0	0.0	5.00293730864571	0.0
33	2.3201490092499702E-4	0.0	0.0	5.138480413766094	0.0
34	2.3201490092499702E-4	0.0	0.0	5.284324980487547	0.0
35	2.3201490092499702E-4	0.0	0.0	5.471282587652909	0.0
36	2.3201490092499702E-4	0.0	0.0	5.647521106395537	0.0
37	2.3201490092499702E-4	0.0	0.0	5.81155564134951	0.0
38	2.3201490092499702E-4	0.0	0.0	5.966216774306113	0.0
39	2.3201490092499702E-4	0.0	0.0	6.122269996668266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGCC	30	2.166389E-6	45.000004	18
CCGTGAT	30	2.166389E-6	45.000004	30
ATTAACG	60	0.0	45.000004	1
CATACGT	30	2.166389E-6	45.000004	37
CTTTCGT	45	3.8562575E-10	45.000004	22
ACGTAAG	135	0.0	45.000004	1
CGACTTA	30	2.166389E-6	45.000004	32
TAGTCGA	45	3.8562575E-10	45.000004	21
TCGTTAC	25	3.891947E-5	45.0	26
ACGCCTA	40	6.8193913E-9	45.0	1
CGTATCG	40	6.8193913E-9	45.0	40
CGAGTCA	25	3.891947E-5	45.0	45
GCGAATC	20	7.034663E-4	45.0	12
GACGTAA	25	3.891947E-5	45.0	27
ATTACGT	25	3.891947E-5	45.0	12
TAAACCG	25	3.891947E-5	45.0	32
GTAGGTT	25	3.891947E-5	45.0	32
CGCGATA	25	3.891947E-5	45.0	11
TCTAGAT	25	3.891947E-5	45.0	44
TACGATG	20	7.034663E-4	45.0	16
>>END_MODULE