FastQCFastQC Report
Thu 26 May 2016
SRR1545297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545297_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3109022
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT38530.12392964732961041No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37940.12203194445069865No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.11260775896728939No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34130.10977728687670914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT351.2128294E-745.00000426
CTTAACG1700.045.01
AACCGAT207.0351455E-445.042
TCGTTAA551.8189894E-1245.043
TCGATTG453.8562575E-1045.01
ATCCGAT1000.045.010
ATTCCGT207.0351455E-445.015
TATTCCG453.8562575E-1045.01
TATTACG253.8923466E-545.01
ACGCTAG453.8562575E-1045.01
TTAACGA453.8562575E-1045.017
CGATCGA502.1827873E-1145.041
TCGACAC207.0351455E-445.034
ATTTCGC207.0351455E-445.011
TTATCCG302.166702E-644.99999614
CGTTAGA302.166702E-644.99999637
AATGCGT302.166702E-644.99999619
TTCGATC302.166702E-644.99999643
TAATCGC302.166702E-644.99999616
ATTACCG1700.042.352941
Produced by FastQC (version 0.11.5)