FastQCFastQC Report
Thu 26 May 2016
SRR1545296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545296_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2585521
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33150.12821400406339767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC551.8189894E-1245.00000431
CGTATAT302.1665583E-645.00000412
ACGTTAG1150.045.0000041
CGTGCAC302.1665583E-645.00000432
ACCGCGA302.1665583E-645.00000434
TAGGTCG600.045.0000041
CGGTTGC302.1665583E-645.00000428
CGTTATT551.8189894E-1245.00000428
GCTTACC302.1665583E-645.00000438
GTATCGC302.1665583E-645.00000421
TATCGGA600.045.00000411
TATCGCG1050.045.0000041
TCGGTGA302.1665583E-645.00000416
ATCGCAC302.1665583E-645.00000444
AAACTCG351.2127202E-745.025
GGTACGA406.8212103E-945.09
TACCCGC351.2127202E-745.041
ACCGGCA406.8212103E-945.029
CTCCGTA207.034925E-445.015
CTCCGAT207.034925E-445.012
Produced by FastQC (version 0.11.5)