##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545296_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2585521
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2781257626606	31.0	31.0	33.0	30.0	34.0
2	31.592225319384372	31.0	31.0	34.0	30.0	34.0
3	31.687341158706506	31.0	31.0	34.0	30.0	34.0
4	30.281088028292945	35.0	33.0	37.0	0.0	37.0
5	32.8363161621971	35.0	33.0	37.0	19.0	37.0
6	34.45730860433932	35.0	33.0	37.0	30.0	37.0
7	35.481759769114234	36.0	35.0	37.0	33.0	37.0
8	35.69760369380098	37.0	35.0	37.0	35.0	37.0
9	37.76666366275888	39.0	37.0	39.0	35.0	39.0
10	37.20359300891387	39.0	37.0	39.0	33.0	39.0
11	36.69583267743716	39.0	35.0	39.0	32.0	39.0
12	35.223079990454536	35.0	35.0	39.0	31.0	39.0
13	34.60957462731883	35.0	33.0	39.0	27.0	39.0
14	35.34279744778712	35.0	33.0	40.0	27.0	41.0
15	35.830413676779266	36.0	34.0	40.0	30.0	41.0
16	36.09920592406714	35.0	34.0	40.0	32.0	41.0
17	36.04114915330411	35.0	34.0	40.0	31.0	41.0
18	36.0499079295817	36.0	35.0	40.0	31.0	41.0
19	36.00395548904844	36.0	35.0	40.0	31.0	41.0
20	35.8746399661809	36.0	34.0	40.0	31.0	41.0
21	35.69070063635144	35.0	34.0	40.0	31.0	41.0
22	35.527436443177216	35.0	34.0	40.0	30.0	41.0
23	35.53046213896542	35.0	34.0	40.0	30.0	41.0
24	35.46077057583365	35.0	34.0	40.0	30.0	41.0
25	35.313838100715486	35.0	34.0	40.0	30.0	41.0
26	35.15979100537184	35.0	34.0	40.0	29.0	41.0
27	35.12589764306691	35.0	34.0	40.0	29.0	41.0
28	35.22916928541675	35.0	34.0	40.0	29.0	41.0
29	35.3352105823159	36.0	34.0	40.0	30.0	41.0
30	35.29963245318835	36.0	34.0	40.0	30.0	41.0
31	35.109718698861855	35.0	34.0	40.0	29.0	41.0
32	34.97839197593058	35.0	34.0	40.0	29.0	41.0
33	34.812542230366724	35.0	34.0	40.0	29.0	41.0
34	34.78079891828378	35.0	34.0	40.0	29.0	41.0
35	34.644183899492596	35.0	33.0	40.0	28.0	41.0
36	34.40745791660559	35.0	33.0	39.0	26.0	41.0
37	34.31955841781985	35.0	33.0	39.0	26.0	41.0
38	34.36566247189638	35.0	33.0	39.0	27.0	41.0
39	34.35181922714996	35.0	33.0	39.0	27.0	41.0
40	34.25399987081907	35.0	33.0	39.0	26.0	41.0
41	34.294317470250675	35.0	33.0	39.0	26.0	41.0
42	34.239776818676006	35.0	33.0	39.0	27.0	41.0
43	34.14182557403324	35.0	33.0	39.0	27.0	41.0
44	34.096655567678624	35.0	33.0	39.0	27.0	41.0
45	33.99309694255046	35.0	33.0	39.0	26.0	41.0
46	33.89305366307216	35.0	33.0	38.0	26.0	41.0
47	33.82535821600366	35.0	33.0	38.0	26.0	40.0
48	33.82887665580748	35.0	33.0	38.0	26.0	40.0
49	33.84094346942067	35.0	33.0	38.0	26.0	40.0
50	33.64520264967873	35.0	33.0	38.0	24.0	40.0
51	33.57724845398664	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	2.0
14	10.0
15	39.0
16	160.0
17	497.0
18	1140.0
19	2232.0
20	4153.0
21	6783.0
22	10490.0
23	15692.0
24	22137.0
25	30218.0
26	38989.0
27	47818.0
28	57361.0
29	69454.0
30	85415.0
31	107668.0
32	139686.0
33	210788.0
34	326849.0
35	463447.0
36	165039.0
37	195982.0
38	257700.0
39	325563.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.901895594737	25.115247565190923	31.413784687883023	12.569072152189056
2	26.214832523116232	27.419541361296236	32.54833358537796	13.81729253020958
3	27.48301019407694	26.768686079130667	31.719022974479806	14.029280752312589
4	22.024381159541925	38.07008336037495	26.7965721415529	13.108963338530222
5	23.367398679028327	32.13611492615995	30.07711018398226	14.419376210829462
6	21.643954932100726	40.948226682359184	26.628482228533436	10.779336157006654
7	89.9562989432304	4.086565144897295	4.10161046845104	1.855525443421268
8	93.04240808719017	1.9855959398511944	3.2194671789554214	1.7525287940032201
9	90.47569135969114	3.311595612644415	4.2872597051039225	1.9254533225605206
10	64.9264113499755	20.045901773762427	6.802072000188743	8.225614876073333
11	58.238552307252576	15.873396503064566	14.025722475276742	11.862328714406111
12	50.21633937608706	20.696911763625202	16.95016207565129	12.136586784636442
13	18.062201003202063	50.17789451333019	20.142168638351805	11.617735845115936
14	11.227176263507433	50.01251198501192	25.67761004455195	13.0827017069287
15	10.012140686538611	19.82695170528493	57.632755641899635	12.528151966276816
16	13.687183356855352	14.298974945475207	53.102798236796374	18.91104346087307
17	14.403093225698033	16.883096288910437	25.219443199262354	43.494367286129176
18	21.474317942109153	22.10858082374887	35.25289487109175	21.164206363050234
19	31.922308888614715	22.068704914792804	24.39755855783032	21.61142763876217
20	34.371989243173815	22.91897841866301	21.651767670809868	21.057264667353312
21	21.79966822934333	29.161201939570404	27.16272658392641	21.876403247159857
22	24.849575772155784	24.656732627582603	21.98806352762171	28.505628072639905
23	20.510179573091843	33.48404441503279	20.884456169568917	25.121319842306445
24	20.04153128131622	22.19564258035421	40.18304241195489	17.579783726374686
25	14.558883876789244	25.96880860762686	37.64912371626454	21.823183799319363
26	15.69675898977421	39.96900431286383	23.88806743399106	20.4461692633709
27	17.256057869961218	38.452482110955586	27.010803625265467	17.280656393817726
28	13.18616247943838	28.98700107251111	41.507572361624604	16.319264086425907
29	13.14253490882495	23.25763356785731	41.33573078694778	22.26410073636996
30	17.80376179501153	31.848822732439615	30.41475973314469	19.93265573940417
31	33.03009335449219	27.627932629439094	20.65823483932252	18.683739176746194
32	36.89144276917495	25.91203088275052	21.953370326522197	15.243156021552329
33	31.78295593035214	27.66761515377365	21.23015051898631	19.319278396887903
34	19.321366950800247	28.567433797675594	26.52192730207954	25.58927194944462
35	19.69525677803429	25.42632606735741	31.555342230830846	23.32307492377745
36	36.62372109915177	22.699602904018185	23.423170803872797	17.253505192957242
37	20.743517457409936	34.38668647440883	27.033390949058234	17.836405119122993
38	19.49405941781173	36.511093895582356	21.020134820022733	22.974711866583174
39	19.295337380744538	35.75302617924975	24.91246445107195	20.039171988933756
40	24.61793967250701	25.731796415500007	20.970705710763905	28.679558201229078
41	15.248570790954705	21.877989001056267	25.813675464248792	37.05976474374024
42	21.432198771543533	25.129712734880126	21.51059689710507	31.927491596471274
43	21.053977128787583	27.098368181886745	24.667523489463054	27.18013119986262
44	19.887094322575606	31.974058613331703	27.206160769918323	20.932686294174367
45	17.210921899299986	40.282326076639876	19.103925282370554	23.402826741689587
46	22.96856223561905	34.08674692644152	23.623014471744767	19.321676366194666
47	22.13406891686434	25.283492185907598	26.233281416008612	26.349157481219454
48	24.494831022451567	20.038978604312245	31.217770035517017	24.248420337719168
49	20.20749396349904	21.30839393685064	34.124379573788026	24.35973252586229
50	18.857785336108275	35.178944591825015	23.887951403218153	22.075318668848563
51	16.51025073863256	36.58276223631523	22.060505406840633	24.846481618211573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	445.0
1	622.0
2	799.0
3	2834.5
4	4870.0
5	3747.5
6	2625.0
7	3146.0
8	3667.0
9	4242.0
10	4817.0
11	4994.0
12	5171.0
13	5215.0
14	5259.0
15	4800.0
16	4341.0
17	4221.0
18	4101.0
19	4037.0
20	3973.0
21	4241.0
22	4509.0
23	5419.5
24	6330.0
25	6827.5
26	8923.5
27	10522.0
28	12751.5
29	14981.0
30	18294.0
31	21607.0
32	31695.5
33	41784.0
34	42775.5
35	43767.0
36	48689.0
37	53611.0
38	79329.5
39	105048.0
40	170245.0
41	235442.0
42	278911.5
43	322381.0
44	328224.0
45	334067.0
46	306408.0
47	278749.0
48	262008.0
49	245267.0
50	219416.5
51	193566.0
52	172135.5
53	150705.0
54	131594.5
55	112484.0
56	101385.5
57	90287.0
58	84553.5
59	78820.0
60	71050.5
61	63281.0
62	53432.5
63	43584.0
64	36764.0
65	29944.0
66	25719.5
67	21495.0
68	17967.0
69	14439.0
70	12006.5
71	9574.0
72	8199.0
73	6824.0
74	4892.0
75	2026.0
76	1092.0
77	889.0
78	686.0
79	470.0
80	254.0
81	148.5
82	43.0
83	30.0
84	17.0
85	10.5
86	4.0
87	4.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2585521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.508660446413483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.93221728622406	11.3069207844194
2	10.015879265447767	2.9063398266930984
3	3.5641573934400395	1.5513344819698702
4	1.7015514792989659	0.9874893058096501
5	0.9371663501064739	0.6798514177749743
6	0.546318514626216	0.4755809894580446
7	0.36461076131967496	0.3703009611766832
8	0.2682606156327688	0.31136817466893457
9	0.18443544411596396	0.2408320109665791
>10	1.7231026797702744	5.808576494704725
>50	0.7384916997488224	7.94404169384476
>100	1.9790866691755231	62.92666212966036
>500	0.03978501447933139	3.506971640890865
>1k	0.0049368266142236035	0.9837300879620342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3315	0.12821400406339767	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07414366388824535	0.0
2	0.0	0.0	0.0	0.24273637692364516	0.0
3	0.0	0.0	0.0	0.33985413384768487	0.0
4	0.0	0.0	0.0	0.5548978329706082	0.0
5	3.867692430268406E-5	0.0	0.0	0.8944812283481743	0.0
6	3.867692430268406E-5	0.0	0.0	1.216234561622203	0.0
7	3.867692430268406E-5	0.0	0.0	1.398286844314937	0.0
8	3.867692430268406E-5	0.0	0.0	1.8575366434850076	0.0
9	3.867692430268406E-5	0.0	0.0	1.983546062863152	0.0
10	3.867692430268406E-5	0.0	0.0	2.1911251155956575	0.0
11	3.867692430268406E-5	0.0	0.0	2.506535433284046	0.0
12	7.735384860536812E-5	0.0	0.0	2.7637756568211977	0.0
13	7.735384860536812E-5	0.0	0.0	2.8707947063667247	0.0
14	1.1603077290805219E-4	0.0	0.0	2.906996307514037	0.0
15	1.1603077290805219E-4	0.0	0.0	2.9645088939521282	0.0
16	1.1603077290805219E-4	0.0	0.0	3.1075361600234537	0.0
17	1.1603077290805219E-4	0.0	0.0	3.2785268423656198	0.0
18	1.1603077290805219E-4	0.0	0.0	3.501112541727567	0.0
19	1.1603077290805219E-4	0.0	0.0	3.622790145583811	0.0
20	1.1603077290805219E-4	0.0	0.0	3.751816365057565	0.0
21	1.1603077290805219E-4	0.0	0.0	3.926868124451513	0.0
22	1.1603077290805219E-4	0.0	0.0	4.099715299160208	0.0
23	1.1603077290805219E-4	0.0	0.0	4.298476013151701	0.0
24	1.1603077290805219E-4	0.0	0.0	4.438602509900326	0.0
25	1.1603077290805219E-4	0.0	0.0	4.554130482792443	0.0
26	1.1603077290805219E-4	0.0	0.0	4.672056424991327	0.0
27	1.1603077290805219E-4	0.0	0.0	4.777218982170325	0.0
28	1.1603077290805219E-4	0.0	0.0	4.890813108847308	0.0
29	1.1603077290805219E-4	0.0	0.0	5.014385881994383	0.0
30	1.1603077290805219E-4	0.0	0.0	5.18653687206563	0.0
31	1.5470769721073624E-4	0.0	0.0	5.35772093902931	0.0
32	1.5470769721073624E-4	0.0	0.0	5.492393989451256	0.0
33	1.5470769721073624E-4	0.0	0.0	5.6298131014986925	0.0
34	1.5470769721073624E-4	0.0	0.0	5.783553875601862	0.0
35	1.5470769721073624E-4	0.0	0.0	6.003664251808436	0.0
36	1.5470769721073624E-4	0.0	0.0	6.167963826246238	0.0
37	1.9338462151342032E-4	0.0	0.0	6.3360537392657035	0.0
38	1.9338462151342032E-4	0.0	0.0	6.480512051536228	0.0
39	1.9338462151342032E-4	0.0	0.0	6.6309652870736695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGC	55	1.8189894E-12	45.000004	31
CGTATAT	30	2.1665583E-6	45.000004	12
ACGTTAG	115	0.0	45.000004	1
CGTGCAC	30	2.1665583E-6	45.000004	32
ACCGCGA	30	2.1665583E-6	45.000004	34
TAGGTCG	60	0.0	45.000004	1
CGGTTGC	30	2.1665583E-6	45.000004	28
CGTTATT	55	1.8189894E-12	45.000004	28
GCTTACC	30	2.1665583E-6	45.000004	38
GTATCGC	30	2.1665583E-6	45.000004	21
TATCGGA	60	0.0	45.000004	11
TATCGCG	105	0.0	45.000004	1
TCGGTGA	30	2.1665583E-6	45.000004	16
ATCGCAC	30	2.1665583E-6	45.000004	44
AAACTCG	35	1.2127202E-7	45.0	25
GGTACGA	40	6.8212103E-9	45.0	9
TACCCGC	35	1.2127202E-7	45.0	41
ACCGGCA	40	6.8212103E-9	45.0	29
CTCCGTA	20	7.034925E-4	45.0	15
CTCCGAT	20	7.034925E-4	45.0	12
>>END_MODULE