FastQCFastQC Report
Thu 26 May 2016
SRR1545295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545295_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3699059
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67330.18201926489953255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAG351.2129021E-745.00000410
ATCTACG650.045.0000041
GCACCTA650.045.0000049
ATCGTAG351.2129021E-745.0000041
TACGATT650.045.0000041
CGTAGCA351.2129021E-745.00000412
TGTTACG700.045.0000041
CGTACTC351.2129021E-745.00000431
CTATTCG351.2129021E-745.00000437
TCGAGTC351.2129021E-745.00000423
ACGTAGC351.2129021E-745.00000411
ACCCGGT351.2129021E-745.00000435
CCCGGTG351.2129021E-745.00000432
GCCCCTA551.8189894E-1245.020
TCGTTAA207.0353184E-445.029
CGAACTA207.0353184E-445.027
GCAAGTA750.045.09
GTCGATC406.8230293E-945.028
CAAGTCG1050.045.01
TGCCAAC406.8230293E-945.044