##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545295_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3699059
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291480076419436	31.0	31.0	33.0	30.0	34.0
2	31.59323600948241	31.0	31.0	34.0	30.0	34.0
3	31.68129732453578	31.0	31.0	34.0	30.0	34.0
4	30.260816602276417	35.0	33.0	37.0	0.0	37.0
5	32.81442226252677	35.0	33.0	37.0	19.0	37.0
6	34.41831449565957	35.0	33.0	37.0	30.0	37.0
7	35.503382346699524	36.0	35.0	37.0	33.0	37.0
8	35.71234495043199	37.0	35.0	37.0	35.0	37.0
9	37.77867235964606	39.0	37.0	39.0	35.0	39.0
10	37.23960228804136	39.0	37.0	39.0	34.0	39.0
11	36.70484304251433	39.0	35.0	39.0	32.0	39.0
12	35.069338715603074	35.0	35.0	39.0	31.0	39.0
13	34.397650591677504	35.0	33.0	39.0	27.0	39.0
14	35.0663276795531	35.0	33.0	40.0	27.0	41.0
15	35.59143852531144	35.0	34.0	40.0	30.0	41.0
16	35.903638736229944	35.0	34.0	40.0	31.0	41.0
17	35.86813511220016	35.0	34.0	40.0	31.0	41.0
18	35.8713872906596	36.0	35.0	40.0	31.0	41.0
19	35.82411770128565	36.0	35.0	40.0	31.0	41.0
20	35.679730980230374	35.0	34.0	40.0	31.0	41.0
21	35.448982024887954	35.0	34.0	40.0	30.0	41.0
22	35.284832169478776	35.0	34.0	40.0	30.0	41.0
23	35.29354465554618	35.0	34.0	40.0	30.0	41.0
24	35.21485464276185	35.0	34.0	40.0	30.0	41.0
25	35.0740772180168	35.0	34.0	39.0	30.0	41.0
26	34.908262074219415	35.0	34.0	39.0	29.0	41.0
27	34.85400881683693	35.0	34.0	39.0	29.0	41.0
28	34.95423701000714	35.0	34.0	39.0	29.0	41.0
29	35.081097922471635	35.0	34.0	39.0	29.0	41.0
30	35.086862361481664	36.0	34.0	39.0	29.0	41.0
31	34.88957894426664	35.0	34.0	39.0	29.0	41.0
32	34.74322307376011	35.0	34.0	39.0	29.0	41.0
33	34.60321881862387	35.0	34.0	39.0	29.0	41.0
34	34.5696940762502	35.0	34.0	39.0	28.0	41.0
35	34.46672761910529	35.0	33.0	39.0	27.0	41.0
36	34.22940266700261	35.0	33.0	39.0	26.0	41.0
37	34.1443802329187	35.0	33.0	39.0	26.0	41.0
38	34.188230033638284	35.0	33.0	39.0	27.0	41.0
39	34.1749412485716	35.0	33.0	39.0	27.0	41.0
40	34.03240202440675	35.0	33.0	39.0	25.0	41.0
41	34.09265004964776	35.0	33.0	39.0	26.0	41.0
42	34.05039687120427	35.0	33.0	38.0	26.0	41.0
43	33.92665621175548	35.0	33.0	38.0	26.0	41.0
44	33.89253212776547	35.0	33.0	38.0	26.0	41.0
45	33.798291943978185	35.0	33.0	38.0	26.0	41.0
46	33.793078996577236	35.0	33.0	38.0	26.0	40.0
47	33.71661414429994	35.0	33.0	38.0	26.0	40.0
48	33.67860664022931	35.0	33.0	38.0	26.0	40.0
49	33.69959954680366	35.0	33.0	38.0	26.0	40.0
50	33.48505227951217	35.0	33.0	38.0	24.0	40.0
51	33.385721341562814	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	3.0
14	24.0
15	70.0
16	275.0
17	765.0
18	1773.0
19	3651.0
20	6664.0
21	10507.0
22	16387.0
23	23830.0
24	33594.0
25	45421.0
26	58522.0
27	71208.0
28	84938.0
29	102561.0
30	125550.0
31	158481.0
32	205461.0
33	311913.0
34	488923.0
35	700557.0
36	218259.0
37	259294.0
38	340822.0
39	429275.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.36745291167294	25.20316653505662	32.52600188318164	11.903378670088799
2	26.965155192171846	27.735999885376255	31.775784057513007	13.523060864938893
3	27.775766755815468	25.868768246194506	32.02044087428722	14.33502412370281
4	21.460295713044857	38.91476183537489	26.58327428678483	13.041668164795425
5	23.41738804382412	31.937122387071952	30.568260738744634	14.077228830359289
6	21.557158185365523	41.04297876838407	26.708630492241404	10.691232554009005
7	89.30682100501777	4.375599307823963	4.449807369928406	1.8677723172298686
8	92.1451915203299	2.39250036293014	3.2148176063155525	2.247490510424408
9	89.86569286945681	3.5564179971176455	4.528232720808184	2.049656412617371
10	67.87177495682009	17.3257036451703	6.962067920517083	7.840453477492519
11	62.16210663306533	14.979728628280869	13.811972179951711	9.046192558702092
12	53.59792855426204	20.914075714931823	15.808777313365372	9.67921841744076
13	18.453260680621746	54.005248361813095	16.724388553953855	10.817102403611296
14	10.995607261198051	53.658078987115374	24.27509266545897	11.071221086227606
15	9.285523696702324	21.660076251825124	58.89376189998592	10.160638151486635
16	10.787067738038242	14.678570955478138	55.940281028229066	18.594080278254552
17	12.342057804430802	15.874685967431176	25.704618390785335	46.07863783735269
18	21.622255822359147	22.35111686512705	34.878167663722046	21.14845964879176
19	33.903595481986095	23.963797279253992	21.177926602414292	20.95468063634562
20	37.52913916755586	22.17972192387307	20.951842076592992	19.33929683197808
21	21.811790512127544	30.508326577110555	25.404109531640344	22.275773379121556
22	22.697799629581468	26.197878974085032	21.103448201285786	30.00087319504771
23	19.15927807585659	34.13362695755867	20.56709557755094	26.139999389033807
24	20.422464199678892	23.241505474770747	40.74768745240343	15.588342873146926
25	14.024512720667609	24.95502234487203	39.40572453697008	21.61474039749028
26	14.894707005214029	41.30109846855646	24.287744531784973	19.51644999444453
27	16.73257982638287	38.53274576047584	27.80901845577483	16.925655957366452
28	11.365971724160119	29.756027140956657	44.1669894965179	14.711011638365326
29	11.405549357282489	23.54190619830611	43.093824672707306	21.958719771704104
30	17.09302284716194	32.84375837206165	31.148705657303655	18.914513123472755
31	33.86169293325681	25.828731036731234	21.031673190397882	19.277902839614075
32	35.152372535826004	26.220560418203657	22.692284713490647	15.934782332479694
33	30.102791007118295	27.312081261747924	22.78065854045583	19.804469190677953
34	17.108594374947792	27.596423847254126	26.961316378030197	28.333665399767888
35	18.69137529301371	23.491217631294877	33.219610717212134	24.59779635847928
36	35.26296823056891	20.722837889311847	25.909697574437175	18.104496305682066
37	19.241163766244334	32.65052003766363	29.486418032261717	18.621898163830313
38	18.42836245650583	35.914458244650874	21.76272397926067	23.89445531958263
39	18.99091093167208	34.40169513381647	26.37865467947389	20.228739255037564
40	25.106979910296108	25.411246481875526	22.420431790896007	27.061341816932362
41	14.514448133971369	21.81741356382799	27.007301046022786	36.66083725617785
42	22.636513772827087	24.501474564206735	21.48900571740002	31.373005945566156
43	21.503522922992037	26.07376632813913	25.150531527072157	27.272179221796677
44	18.88061260985564	31.858345595460904	27.595153253841044	21.665888540842417
45	15.249040364049343	41.98513730113523	19.615204839933615	23.150617494881807
46	21.97834638485085	35.15610321435803	24.005456522861625	18.860093877929497
47	20.450498356473904	26.42726163599986	26.680866674470455	26.441373333055786
48	23.8284385299072	19.603120685558135	32.86352015472043	23.70492062981423
49	19.39030980581818	19.933718278080992	34.87378817153227	25.802183744568552
50	17.95710746976461	35.82565187524719	25.109439995415052	21.10780065957315
51	14.924119891031745	39.388395805527836	21.24210508672611	24.445379216714304
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1118.0
1	1197.5
2	1277.0
3	3645.5
4	6014.0
5	4746.0
6	3478.0
7	4296.0
8	5114.0
9	5990.5
10	6867.0
11	7359.5
12	7852.0
13	8191.0
14	8530.0
15	7856.5
16	7183.0
17	6910.5
18	6638.0
19	7593.0
20	8548.0
21	8278.0
22	8008.0
23	9347.5
24	10687.0
25	11858.0
26	16242.5
27	19456.0
28	23833.5
29	28211.0
30	30881.0
31	33551.0
32	43570.5
33	53590.0
34	59409.5
35	65229.0
36	74973.5
37	84718.0
38	122242.5
39	159767.0
40	256136.0
41	352505.0
42	428616.0
43	504727.0
44	496939.0
45	489151.0
46	434484.5
47	379818.0
48	354492.0
49	329166.0
50	300065.5
51	270965.0
52	247831.5
53	224698.0
54	190332.0
55	155966.0
56	135139.5
57	114313.0
58	107819.5
59	101326.0
60	93546.5
61	85767.0
62	75449.5
63	65132.0
64	51225.0
65	37318.0
66	28138.5
67	18959.0
68	15594.5
69	12230.0
70	10580.0
71	8930.0
72	7272.0
73	5614.0
74	4074.0
75	1628.5
76	723.0
77	512.5
78	302.0
79	165.5
80	29.0
81	21.5
82	14.0
83	9.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3699059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.389475242739628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17262510169792	10.199114779736716
2	10.611504196452248	2.8416494545325013
3	4.116513668097326	1.6535387353616533
4	2.173282970607605	1.1639647412167264
5	1.258392069998806	0.8424604733454444
6	0.809142137722548	0.6500393172536085
7	0.5713271501454594	0.5354839512664331
8	0.3763356219517599	0.40311491944672884
9	0.2746019874632763	0.33090988614719724
>10	1.5463278142002186	4.103270915856061
>50	0.40234436266889845	3.9804987863236327
>100	1.4458586826716477	51.63064509419728
>500	0.22529158865966423	18.442167974063842
>1k	0.016233279027083887	3.025377213807423
>5k	2.1936863550113363E-4	0.19776375744473723
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6733	0.18201926489953255	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10410755816546857	0.0
2	0.0	0.0	0.0	0.3629301398004195	0.0
3	0.0	0.0	0.0	0.5069667718195358	0.0
4	0.0	0.0	0.0	0.8291838545965339	0.0
5	0.0	0.0	0.0	1.3101980801063189	0.0
6	2.703390240599028E-5	0.0	0.0	1.8096223931545834	0.0
7	2.703390240599028E-5	0.0	0.0	2.050791836518423	0.0
8	2.703390240599028E-5	0.0	0.0	2.6206394653342917	0.0
9	2.703390240599028E-5	0.0	0.0	2.778355251970839	0.0
10	2.703390240599028E-5	0.0	0.0	3.028229611909407	0.0
11	2.703390240599028E-5	0.0	0.0	3.4197886543577702	0.0
12	2.703390240599028E-5	0.0	0.0	3.7199460727714806	0.0
13	2.703390240599028E-5	0.0	0.0	3.845951091885801	0.0
14	5.406780481198056E-5	0.0	0.0	3.8976399132860546	0.0
15	5.406780481198056E-5	0.0	0.0	3.9771195863596662	0.0
16	8.110170721797084E-5	0.0	0.0	4.166167665884756	0.0
17	8.110170721797084E-5	0.0	0.0	4.370949476610132	0.0
18	8.110170721797084E-5	0.0	0.0	4.642829433107177	0.0
19	8.110170721797084E-5	0.0	0.0	4.77926953855021	0.0
20	8.110170721797084E-5	0.0	0.0	4.923927950324663	0.0
21	8.110170721797084E-5	0.0	0.0	5.126033404711847	0.0
22	8.110170721797084E-5	0.0	0.0	5.308160805221004	0.0
23	8.110170721797084E-5	0.0	0.0	5.539922450547558	0.0
24	8.110170721797084E-5	0.0	0.0	5.686662472807273	0.0
25	8.110170721797084E-5	0.0	0.0	5.80696333851393	0.0
26	8.110170721797084E-5	0.0	0.0	5.932725052506597	0.0
27	1.0813560962396111E-4	0.0	0.0	6.039536000912665	0.0
28	1.0813560962396111E-4	0.0	0.0	6.162053646616613	0.0
29	1.0813560962396111E-4	0.0	0.0	6.278677901596055	0.0
30	1.0813560962396111E-4	0.0	0.0	6.467212336975431	0.0
31	1.0813560962396111E-4	0.0	0.0	6.6396345665208365	0.0
32	1.351695120299514E-4	0.0	0.0	6.774425603917105	0.0
33	1.351695120299514E-4	0.0	0.0	6.905837403512623	0.0
34	1.351695120299514E-4	0.0	0.0	7.067446072095633	0.0
35	1.351695120299514E-4	0.0	0.0	7.283257715002653	0.0
36	1.6220341443594168E-4	0.0	0.0	7.450327231871674	0.0
37	1.6220341443594168E-4	0.0	0.0	7.612098103869119	0.0
38	1.6220341443594168E-4	0.0	0.0	7.7582433802759025	0.0
39	1.8923731684193195E-4	0.0	0.0	7.906037724729451	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAG	35	1.2129021E-7	45.000004	10
ATCTACG	65	0.0	45.000004	1
GCACCTA	65	0.0	45.000004	9
ATCGTAG	35	1.2129021E-7	45.000004	1
TACGATT	65	0.0	45.000004	1
CGTAGCA	35	1.2129021E-7	45.000004	12
TGTTACG	70	0.0	45.000004	1
CGTACTC	35	1.2129021E-7	45.000004	31
CTATTCG	35	1.2129021E-7	45.000004	37
TCGAGTC	35	1.2129021E-7	45.000004	23
ACGTAGC	35	1.2129021E-7	45.000004	11
ACCCGGT	35	1.2129021E-7	45.000004	35
CCCGGTG	35	1.2129021E-7	45.000004	32
GCCCCTA	55	1.8189894E-12	45.0	20
TCGTTAA	20	7.0353184E-4	45.0	29
CGAACTA	20	7.0353184E-4	45.0	27
GCAAGTA	75	0.0	45.0	9
GTCGATC	40	6.8230293E-9	45.0	28
CAAGTCG	105	0.0	45.0	1
TGCCAAC	40	6.8230293E-9	45.0	44
>>END_MODULE