##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545294_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3224720
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21553220124538	31.0	31.0	33.0	30.0	34.0
2	31.53574635937384	31.0	31.0	34.0	30.0	34.0
3	31.651745888015082	31.0	31.0	34.0	30.0	34.0
4	30.29284589049592	35.0	33.0	37.0	0.0	37.0
5	32.83263570170433	35.0	33.0	37.0	19.0	37.0
6	34.45108629586445	35.0	33.0	37.0	30.0	37.0
7	35.449919372844775	36.0	35.0	37.0	33.0	37.0
8	35.629638852365474	37.0	35.0	37.0	35.0	37.0
9	37.624701989630104	39.0	37.0	39.0	35.0	39.0
10	37.07316604232305	39.0	37.0	39.0	33.0	39.0
11	36.6432170855144	39.0	35.0	39.0	32.0	39.0
12	35.707755091914954	37.0	35.0	39.0	31.0	39.0
13	35.32606644918008	37.0	35.0	39.0	30.0	39.0
14	36.1756586618373	38.0	35.0	40.0	30.0	41.0
15	36.45218468580218	38.0	35.0	40.0	31.0	41.0
16	36.5772904314173	38.0	35.0	40.0	31.0	41.0
17	36.51244139026024	38.0	35.0	40.0	31.0	41.0
18	36.48852024361805	38.0	35.0	40.0	31.0	41.0
19	36.47218053040264	38.0	35.0	40.0	31.0	41.0
20	36.39966570741026	38.0	35.0	40.0	31.0	41.0
21	36.2271065394825	38.0	35.0	40.0	31.0	41.0
22	36.09692004267037	37.0	34.0	40.0	30.0	41.0
23	36.030302165769434	37.0	34.0	40.0	30.0	41.0
24	35.96819723883004	37.0	34.0	40.0	30.0	41.0
25	35.852999640278846	37.0	34.0	40.0	30.0	41.0
26	35.692234364533974	37.0	34.0	40.0	30.0	41.0
27	35.68290208142102	37.0	34.0	40.0	30.0	41.0
28	35.679250911707065	37.0	34.0	40.0	30.0	41.0
29	35.722355119204146	37.0	34.0	40.0	30.0	41.0
30	35.664494901882954	36.0	34.0	40.0	30.0	41.0
31	35.58105447914858	37.0	34.0	40.0	29.0	41.0
32	35.495778238110596	36.0	34.0	40.0	29.0	41.0
33	35.374897045324865	36.0	34.0	40.0	29.0	41.0
34	35.27206827259421	36.0	34.0	40.0	29.0	41.0
35	35.17000359721154	36.0	34.0	40.0	28.0	41.0
36	35.006288297898735	36.0	34.0	40.0	27.0	41.0
37	34.95982410875983	36.0	33.0	40.0	27.0	41.0
38	34.9264385745119	36.0	33.0	40.0	27.0	41.0
39	34.871494889478775	36.0	33.0	40.0	27.0	41.0
40	34.74157663301	36.0	33.0	40.0	27.0	41.0
41	34.68755643900866	35.0	33.0	40.0	26.0	41.0
42	34.62275019226475	35.0	33.0	40.0	27.0	41.0
43	34.56483601677045	35.0	33.0	40.0	27.0	41.0
44	34.47617312510854	35.0	33.0	40.0	26.0	41.0
45	34.37905058423677	35.0	33.0	40.0	26.0	41.0
46	34.29654109504081	35.0	33.0	39.0	26.0	41.0
47	34.222587077327645	35.0	33.0	39.0	26.0	41.0
48	34.110166153960655	35.0	33.0	39.0	26.0	41.0
49	34.05211150115359	35.0	33.0	39.0	26.0	41.0
50	33.91096405269295	35.0	33.0	39.0	25.0	41.0
51	33.82771031283336	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	13.0
14	22.0
15	70.0
16	241.0
17	635.0
18	1515.0
19	2861.0
20	5122.0
21	8336.0
22	12418.0
23	18368.0
24	25667.0
25	34837.0
26	45427.0
27	56429.0
28	68295.0
29	82623.0
30	102451.0
31	127893.0
32	160989.0
33	229858.0
34	339472.0
35	440170.0
36	254222.0
37	318142.0
38	405460.0
39	482894.0
40	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.32934952491999	25.399538564588553	29.751203205239523	12.519908705251929
2	28.325404996402785	27.093236001885433	31.177807685628522	13.403551316083256
3	29.532362499689896	26.512410379815922	30.060408345530774	13.894818774963408
4	23.82613064079982	38.40752065295592	25.506214493041256	12.260134213203006
5	24.166222183631447	33.15633605398298	27.97942146915081	14.698020293234762
6	22.495224391575082	41.34895432781761	25.57620506586618	10.579616214741124
7	89.03582326527575	4.599345059416011	4.4593329033218385	1.905498771986405
8	91.01376243518817	3.1816405765461813	3.77331985412687	2.031277134138778
9	86.69317025974348	5.300677268103898	5.178092981716242	2.828059490436379
10	56.04458681683992	23.158382743307946	10.0010543551068	10.795976084745343
11	47.769852886452156	20.730730109901014	16.973504676375004	14.525912327271826
12	43.42662308665559	21.57517551911484	19.951716738197426	15.046484656032153
13	24.087858790840755	36.73205735691781	20.430642040239153	18.74944181200228
14	16.89777717135131	38.44867151256543	25.12708080081371	19.52647051526954
15	18.057195663499467	23.948435833188615	41.49439331166737	16.499975191644545
16	19.772259296931207	19.384659753404947	40.83635168324692	20.00672926641693
17	20.032871070976704	21.58661837306805	25.056314966880844	33.3241955890744
18	24.496111290282567	25.382916966434294	28.092330496911362	22.028641246371777
19	32.1455506214493	25.93881019127242	21.095381924632218	20.82025726264606
20	34.56340395445186	25.014047731275895	21.96221687464338	18.460331439628867
21	26.511790170929565	28.065630504353866	23.996222927882112	21.426356396834453
22	25.715100845964923	25.468815897194176	22.883444143987695	25.932639112853206
23	24.72859659133196	29.309335384157386	23.07837579696842	22.883692227542234
24	24.160640303654272	23.19404475427324	33.466905653824206	19.178409288248282
25	21.323339700811232	25.787975390111388	30.455822521025084	22.432862388052296
26	20.59127614180456	33.740014636929715	23.281897343025133	22.386811878240593
27	21.780092535165842	32.01022104244709	25.98963630950904	20.22005011287802
28	18.384603934605177	28.92877521149123	33.656255426827755	19.03036542707584
29	19.634945049492668	25.75383289091766	31.825584856979834	22.78563720260984
30	22.335396561561936	29.81198367610211	26.463817013570168	21.388802748765784
31	30.330385273760204	26.786232603140736	21.326471755687315	21.556910367411746
32	31.963488302860405	26.21781115879828	22.3005408221489	19.51815971619241
33	28.926790543054903	27.75524696717855	22.110508819370363	21.20745367039619
34	21.177838696072836	27.142573618794806	26.686099878439055	24.99348780669329
35	23.32394750551986	24.66586246247736	27.87634275223895	24.133847279763824
36	29.5984457565308	27.16986280979434	22.46272544593019	20.76896598774467
37	23.414808107370565	32.33710213599941	25.060253293309188	19.187836463320846
38	22.77943511374631	31.906677168870473	21.854114465752065	23.459773251631148
39	24.0100845964921	30.397181770820414	24.30018730308368	21.29254632960381
40	26.21715993946761	25.576639212086633	23.664318142350343	24.541882706095414
41	18.596033143962888	24.589328685901414	25.93301123818502	30.881626931950677
42	22.985251432682528	25.804503956932695	22.55898186509216	28.651262745292616
43	22.8704507678186	26.34817906670967	23.956777642710065	26.824592522761666
44	21.331991614775855	28.922821206182242	25.997109826589593	23.748077352452306
45	20.80261231982932	33.08172492495473	21.182211168721626	24.933451586494332
46	22.245249199930537	32.19287255947803	23.698739735542933	21.8631385050485
47	22.911787690094023	25.60854895928949	26.999274355602964	24.48038899501352
48	24.08556401796125	21.810482770597137	30.100721923143713	24.0032312882979
49	22.093856210771786	24.045808628346027	29.673491031779502	24.186844129102685
50	19.589173633679824	30.666197375275996	26.083939070679	23.66068992036518
51	18.984842094817534	31.809831551266466	24.07210548512739	25.13322086878861
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	1178.5
2	1903.0
3	5308.5
4	8714.0
5	6446.0
6	4178.0
7	4360.0
8	4542.0
9	4800.5
10	5059.0
11	5176.0
12	5293.0
13	5463.0
14	5633.0
15	5287.0
16	4941.0
17	5050.5
18	5160.0
19	5217.5
20	5275.0
21	6012.0
22	6749.0
23	6918.0
24	7087.0
25	11319.5
26	18764.0
27	21976.0
28	30582.5
29	39189.0
30	44827.5
31	50466.0
32	55771.5
33	61077.0
34	66570.0
35	72063.0
36	72973.5
37	73884.0
38	97422.0
39	120960.0
40	162829.5
41	204699.0
42	242690.0
43	280681.0
44	284015.0
45	287349.0
46	269435.5
47	251522.0
48	244812.0
49	238102.0
50	222301.0
51	206500.0
52	197244.5
53	187989.0
54	177414.0
55	166839.0
56	165062.5
57	163286.0
58	168959.5
59	174633.0
60	165239.5
61	155846.0
62	140704.5
63	125563.0
64	108277.5
65	90992.0
66	76285.0
67	61578.0
68	50399.0
69	39220.0
70	35364.5
71	31509.0
72	25291.5
73	19074.0
74	13828.0
75	7292.5
76	6003.0
77	4147.0
78	2291.0
79	1868.5
80	1446.0
81	995.5
82	545.0
83	377.5
84	210.0
85	130.0
86	50.0
87	41.5
88	33.0
89	25.5
90	18.0
91	10.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3224720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.65451359236878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1452804682405	12.233263552695414
2	9.04255740231828	2.831136755287329
3	3.1904866657847615	1.498365506273967
4	1.5694300536499093	0.9827465642853828
5	0.8831850504435086	0.6912916188372407
6	0.5809726536230777	0.5456906581762219
7	0.40053546218546693	0.4389131485905672
8	0.3080693546444704	0.38581407197393125
9	0.22814319240964456	0.32143236359248684
>10	2.440864695471863	9.392822926536063
>50	1.2587249637188538	14.401127175427986
>100	1.93702965162596	53.90351982197341
>500	0.011040289412805458	1.1299377874578063
>1k	0.003463620207938967	1.060365095209032
>5k	2.1647626299618544E-4	0.18357295368319998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5417	0.1679835768686894	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4088	0.1267706963705376	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3973	0.12320449527400829	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05761740554218661	0.0
2	0.0	0.0	0.0	0.21000272891909996	0.0
3	0.0	0.0	0.0	0.3003671636607209	0.0
4	0.0	0.0	0.0	0.4518221737081049	0.0
5	0.0	0.0	0.0	0.7770907241558958	0.0
6	0.0	0.0	0.0	1.0588826316703466	0.0
7	0.0	0.0	0.0	1.2269592398719888	0.0
8	0.0	0.0	0.0	1.5957664541417549	0.0
9	0.0	0.0	0.0	1.707249001463693	0.0
10	0.0	0.0	0.0	1.8929395420377584	0.0
11	0.0	0.0	0.0	2.19290356992235	0.0
12	0.0	0.0	0.0	2.4220087325411197	0.0
13	0.0	0.0	0.0	2.5258006896722818	0.0
14	3.101044431764618E-5	0.0	0.0	2.5688741968294924	0.0
15	3.101044431764618E-5	0.0	0.0	2.642244908085043	0.0
16	3.101044431764618E-5	0.0	0.0	2.793017688357439	0.0
17	3.101044431764618E-5	0.0	0.0	2.9678855838646454	0.0
18	3.101044431764618E-5	0.0	0.0	3.16030539085564	0.0
19	3.101044431764618E-5	0.0	0.0	3.2808739983626487	3.101044431764618E-5
20	6.202088863529236E-5	0.0	0.0	3.4017527103128335	3.101044431764618E-5
21	6.202088863529236E-5	0.0	0.0	3.565487856310005	3.101044431764618E-5
22	6.202088863529236E-5	3.101044431764618E-5	0.0	3.7432707335830706	3.101044431764618E-5
23	6.202088863529236E-5	3.101044431764618E-5	0.0	3.930790890371877	3.101044431764618E-5
24	6.202088863529236E-5	3.101044431764618E-5	0.0	4.084478652410132	3.101044431764618E-5
25	6.202088863529236E-5	3.101044431764618E-5	0.0	4.212396735220422	3.101044431764618E-5
26	6.202088863529236E-5	3.101044431764618E-5	0.0	4.330422486293384	3.101044431764618E-5
27	6.202088863529236E-5	3.101044431764618E-5	0.0	4.447548934481134	3.101044431764618E-5
28	6.202088863529236E-5	3.101044431764618E-5	0.0	4.575498027735741	3.101044431764618E-5
29	6.202088863529236E-5	3.101044431764618E-5	0.0	4.704439455208514	3.101044431764618E-5
30	6.202088863529236E-5	3.101044431764618E-5	0.0	4.8686087474261335	3.101044431764618E-5
31	6.202088863529236E-5	3.101044431764618E-5	0.0	5.021645290133717	3.101044431764618E-5
32	6.202088863529236E-5	3.101044431764618E-5	0.0	5.169813193083431	3.101044431764618E-5
33	6.202088863529236E-5	3.101044431764618E-5	0.0	5.317857054255874	3.101044431764618E-5
34	6.202088863529236E-5	3.101044431764618E-5	0.0	5.467978615197598	3.101044431764618E-5
35	6.202088863529236E-5	3.101044431764618E-5	0.0	5.651994591778511	3.101044431764618E-5
36	6.202088863529236E-5	3.101044431764618E-5	0.0	5.82459872485053	3.101044431764618E-5
37	1.2404177727058473E-4	3.101044431764618E-5	0.0	5.9965826490361955	3.101044431764618E-5
38	1.2404177727058473E-4	3.101044431764618E-5	0.0	6.167295145004838	3.101044431764618E-5
39	1.2404177727058473E-4	3.101044431764618E-5	0.0	6.3458532833858445	3.101044431764618E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCTCG	25	3.892379E-5	45.0	27
CCGTAAA	25	3.892379E-5	45.0	28
AATCTCG	45	3.8562575E-10	45.0	18
TCGACTA	45	3.8562575E-10	45.0	14
GTACGAT	20	7.0351845E-4	45.0	20
ATTACGC	30	2.1667274E-6	44.999996	22
CAATCGT	30	2.1667274E-6	44.999996	20
TCGAACG	100	0.0	40.5	1
GACTAAT	740	0.0	40.43919	9
TAGGGTA	1170	0.0	40.384613	5
GGACTAA	890	0.0	40.19663	8
ATTACGG	185	0.0	40.135136	2
TCGTTAG	45	1.9301297E-8	40.0	1
CGGTGAT	45	1.9301297E-8	40.0	41
ACGGTAC	45	1.9301297E-8	40.0	13
GGCGATT	1585	0.0	39.463722	8
AGTACGG	400	0.0	38.8125	2
GTAGGGT	665	0.0	38.57143	4
TACGCCC	35	6.2517993E-6	38.57143	25
ACGTAGG	280	0.0	38.57143	2
>>END_MODULE