FastQCFastQC Report
Thu 26 May 2016
SRR1545293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545293_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1568550
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27540.175576169073348No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25540.16282553951101336No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23910.15243377641771064No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19010.12119473398999075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.1660107E-645.0000041
GTCGTTT302.1660107E-645.0000049
TATGTCG302.1660107E-645.00000434
TCGCGAT302.1660107E-645.00000438
CCCGCAT302.1660107E-645.00000423
TAGGTCG351.2123019E-745.01
CGCATCG351.2123019E-745.021
CCAATCG207.0340745E-445.023
ATTAACG551.8189894E-1245.01
ATTCGAT502.1827873E-1145.014
CGTCTAG207.0340745E-445.015
TATGCGA207.0340745E-445.011
CACCTCG207.0340745E-445.043
CGTAGGT207.0340745E-445.024
TCGACCG207.0340745E-445.024
ATGTTCG253.8914593E-545.01
TGTCGTA253.8914593E-545.023
TACGTAG800.045.01
CTATGCG1050.042.8571431
CGTACAT700.041.78571335