FastQCFastQC Report
Thu 26 May 2016
SRR1545292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545292_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5233590
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC65930.1259747133420845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG302.1669875E-645.00000431
CGCCTAT302.1669875E-645.00000427
TATCGCC302.1669875E-645.00000431
CGAACCG351.2130295E-745.029
GTCGATA406.8230293E-945.012
CTCCGCG406.8230293E-945.025
ATTACCG1750.045.01
CAATTCG453.8562575E-1045.023
TGACGTA351.2130295E-745.029
TACGCAC253.8927137E-545.018
CTCGCTA453.8562575E-1045.038
TACTCGC1600.045.045
TCGGTGA253.8927137E-545.031
GTTAGCG4000.043.8751
CGCTAAG1050.042.85714726
ATCGGCC950.042.6315827
GTCGGTA750.042.015
CTATACG1400.041.7857131
ACGTAGA700.041.78571327
CGTTTTT50650.041.5794681
Produced by FastQC (version 0.11.5)