##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545292_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5233590
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340731123378024	31.0	31.0	33.0	30.0	34.0
2	31.666345472228432	31.0	31.0	34.0	30.0	34.0
3	31.75090005139875	31.0	31.0	34.0	30.0	34.0
4	30.28193381598482	35.0	33.0	37.0	0.0	37.0
5	32.88617507294228	35.0	33.0	37.0	19.0	37.0
6	34.54552668436007	35.0	33.0	37.0	30.0	37.0
7	35.56422264640524	37.0	35.0	37.0	33.0	37.0
8	35.7679447568495	37.0	35.0	37.0	35.0	37.0
9	37.80419157786529	39.0	37.0	39.0	35.0	39.0
10	37.19094598545167	39.0	37.0	39.0	33.0	39.0
11	36.772294352442586	39.0	35.0	39.0	32.0	39.0
12	35.87067137471602	37.0	35.0	39.0	32.0	39.0
13	35.524808210043204	37.0	35.0	39.0	31.0	39.0
14	36.398924065507615	38.0	35.0	40.0	31.0	41.0
15	36.6788095743075	38.0	35.0	40.0	31.0	41.0
16	36.83261738118576	38.0	35.0	40.0	32.0	41.0
17	36.77109383807291	38.0	35.0	40.0	32.0	41.0
18	36.728958898194165	38.0	35.0	40.0	32.0	41.0
19	36.74614824623251	38.0	35.0	40.0	32.0	41.0
20	36.675816026857284	38.0	35.0	40.0	32.0	41.0
21	36.51059846109458	38.0	35.0	40.0	31.0	41.0
22	36.409474949317776	38.0	35.0	40.0	31.0	41.0
23	36.345672282314816	38.0	35.0	40.0	31.0	41.0
24	36.300716143221	38.0	35.0	40.0	31.0	41.0
25	36.20345976662291	37.0	35.0	40.0	31.0	41.0
26	36.08497627823349	37.0	35.0	40.0	31.0	41.0
27	36.07662961752831	37.0	34.0	40.0	30.0	41.0
28	36.1401546548354	37.0	35.0	40.0	31.0	41.0
29	36.19724051750328	37.0	35.0	40.0	31.0	41.0
30	36.15095851986877	37.0	35.0	40.0	30.0	41.0
31	36.0415936288475	37.0	35.0	40.0	30.0	41.0
32	35.92543970773408	37.0	34.0	40.0	30.0	41.0
33	35.825026415901895	37.0	34.0	40.0	30.0	41.0
34	35.751586578237884	37.0	34.0	40.0	30.0	41.0
35	35.63055569886063	37.0	34.0	40.0	30.0	41.0
36	35.49057071723234	37.0	34.0	40.0	29.0	41.0
37	35.458807052138205	37.0	34.0	40.0	29.0	41.0
38	35.448776652355264	37.0	34.0	40.0	29.0	41.0
39	35.4010207142707	37.0	34.0	40.0	29.0	41.0
40	35.31651161057706	37.0	34.0	40.0	29.0	41.0
41	35.31999602567262	36.0	34.0	40.0	29.0	41.0
42	35.272996547303094	36.0	34.0	40.0	29.0	41.0
43	35.21657179870796	36.0	34.0	40.0	29.0	41.0
44	35.133915725152335	36.0	34.0	40.0	28.0	41.0
45	35.051567279821306	36.0	34.0	40.0	28.0	41.0
46	35.0334972743375	36.0	34.0	40.0	28.0	41.0
47	34.99244514759467	36.0	34.0	40.0	28.0	41.0
48	34.90651006288227	36.0	34.0	40.0	28.0	41.0
49	34.892067204347306	36.0	34.0	40.0	28.0	41.0
50	34.73329741152823	35.0	34.0	40.0	27.0	41.0
51	34.646967951253345	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	0.0
12	7.0
13	6.0
14	20.0
15	68.0
16	236.0
17	651.0
18	1558.0
19	3088.0
20	5699.0
21	9548.0
22	15100.0
23	22627.0
24	32597.0
25	45529.0
26	60504.0
27	75590.0
28	93170.0
29	115510.0
30	146737.0
31	188029.0
32	243293.0
33	353523.0
34	531676.0
35	743380.0
36	384959.0
37	499220.0
38	700238.0
39	960467.0
40	555.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.68736947296215	25.594802038371366	31.032484394077485	11.685344094588991
2	28.538307356900326	26.805233119140016	31.18274454055438	13.473714983405271
3	28.30991728431154	26.215007289451407	31.270695641041808	14.204379785195249
4	21.879952384500886	39.1460928349374	26.07067041934886	12.903284361212858
5	22.801671510378156	33.407355180669484	29.491286096159612	14.299687212792747
6	21.42026792316555	40.965398512302265	26.6816850383771	10.932648526155086
7	90.06223261661688	4.174495900519529	3.973620402056714	1.7896510808068649
8	92.88668008002155	2.10027915828332	3.1203246719746867	1.8927160897204403
9	88.98303076855466	4.401510244402026	4.542904583660547	2.072554403382764
10	52.235234322902635	29.061122479980284	9.461287567425037	9.242355629692046
11	43.89371349303251	21.214672910946405	22.487680540508524	12.403933055512564
12	40.55470527878569	23.46515489367719	21.883047774090063	14.09709205344706
13	19.88090393018941	41.22437944126307	23.562774309795	15.33194231875252
14	14.705775576611849	42.947365001843856	26.897273191060055	15.449586230484238
15	13.424761970272794	26.21829375247201	46.179066376999344	14.177877900255847
16	15.934874531631252	22.62001799911724	43.70050003917005	17.744607430081455
17	17.05162230896956	22.677664853379802	26.231745322044713	34.038967515605925
18	21.127352352782697	26.615592738445308	32.52717541878519	19.729879489986796
19	29.096681245569485	25.82269532003844	24.78358067789032	20.297042756501753
20	31.220825475438467	26.08811542363846	24.38773384999589	18.303325250927184
21	22.244654243072155	29.99556709639081	28.306669035977215	19.453109624559815
22	23.444977539318135	26.229700836328405	24.833145126003377	25.492176498350084
23	19.29650584015943	32.58367583245917	25.480826736523117	22.638991590858282
24	18.473304175527698	25.649200644299615	37.99890705997222	17.878588120200476
25	16.944219933162515	28.475807237479433	34.68823503560653	19.891737793751517
26	17.12797525216916	37.26936959142768	25.98294860697915	19.61970654942401
27	17.90925540594506	35.40395789505865	28.929931461960145	17.756855237036145
28	14.778249729153412	30.021018077457345	37.55924709425079	17.64148509913845
29	15.544702584650308	26.717759702231163	37.368727775771504	20.36880993734702
30	18.582903895796193	31.802414786026418	30.187595894978397	19.42708542319899
31	28.390110803482887	27.924254670312347	24.189915526435964	19.4957189997688
32	30.234294241619995	27.33037933808342	25.35229163919986	17.083034781096725
33	27.371861380046962	28.185528480450323	24.797280642923884	19.64532949657883
34	18.58684000848366	27.39742700517236	29.389214669089476	24.626518317254504
35	19.64758416306971	26.63502490642179	31.900798495869946	21.816592434638558
36	29.15503507152834	25.428549045683745	27.067424081748857	18.34899180103906
37	20.06389495547034	32.273162399041574	28.84094856494299	18.821994080545092
38	19.184020911076335	34.208793581461286	25.28115500067831	21.326030506784065
39	19.50964825292008	32.90087683597683	27.502020601537374	20.087454309565707
40	23.990568615424593	26.705129748413615	26.175397767115882	23.128903869045914
41	17.30892179173378	25.033925087750475	28.16309645960039	29.49405666091536
42	21.46620197608143	26.25280161418835	25.477903313022228	26.803093096707997
43	20.54985201362736	27.826310429361108	26.45146066084657	25.172376896164966
44	19.40574252090821	31.05615839223172	28.87075602024614	20.66734306661393
45	17.21949942582434	35.69297556744032	23.880452996891236	23.207072009844104
46	20.490504605825063	33.40431711310974	26.62103833124108	19.484139949824115
47	20.889695218769525	27.1171604959502	27.683941615602293	24.309202669677983
48	21.64881085449949	24.147229721854405	32.09458517002669	22.109374253619407
49	19.907864391364242	24.576820117739448	32.877718736087466	22.637596754808843
50	18.195636264972993	33.66591574808114	27.153579091980838	20.984868894965025
51	17.571953477440914	34.381103601925254	25.131983972760572	22.914958947873256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	971.0
1	1592.0
2	2213.0
3	6231.0
4	10249.0
5	8144.5
6	6040.0
7	6676.5
8	7313.0
9	8097.0
10	8881.0
11	9392.0
12	9903.0
13	9879.5
14	9856.0
15	10549.5
16	11243.0
17	11409.0
18	11575.0
19	12956.0
20	14337.0
21	16776.5
22	19216.0
23	20888.5
24	22561.0
25	28153.0
26	48390.5
27	63036.0
28	76983.5
29	90931.0
30	102355.0
31	113779.0
32	129925.0
33	146071.0
34	163756.5
35	181442.0
36	199170.0
37	216898.0
38	258156.0
39	299414.0
40	370705.0
41	441996.0
42	507343.5
43	572691.0
44	557470.5
45	542250.0
46	516117.0
47	489984.0
48	464761.0
49	439538.0
50	410357.0
51	381176.0
52	342828.0
53	304480.0
54	256339.5
55	208199.0
56	184246.0
57	160293.0
58	147577.5
59	134862.0
60	118515.0
61	102168.0
62	87536.0
63	72904.0
64	58155.0
65	43406.0
66	35014.0
67	26622.0
68	20817.5
69	15013.0
70	11180.0
71	7347.0
72	6442.5
73	5538.0
74	4297.5
75	2081.5
76	1106.0
77	859.5
78	613.0
79	381.5
80	150.0
81	195.0
82	240.0
83	239.0
84	238.0
85	130.5
86	23.0
87	19.0
88	15.0
89	8.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5233590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.172298302386576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31091546340558	9.920178439097961
2	10.812423394794108	2.848489326558626
3	4.108668534098217	1.6236182267031323
4	2.118157094265278	1.1160398838791439
5	1.2350711293257002	0.8134362670071795
6	0.8088085843679146	0.6392320765695101
7	0.5246218167326985	0.48373325461601585
8	0.3955794392097965	0.4168552300449788
9	0.28968111768065563	0.3434189485192511
>10	1.8357880480588704	5.103755794951393
>50	0.569118048447003	5.491739059957599
>100	1.8904879554488905	62.33153684613298
>500	0.09447637954841998	7.4500029186592265
>1k	0.0060439434727945435	1.279835820592004
>5k	1.5905114402090903E-4	0.1381279067110248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6593	0.1259747133420845	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07864582437676623	0.0
2	0.0	0.0	0.0	0.24923618395785685	0.0
3	0.0	0.0	0.0	0.3604218137072258	0.0
4	0.0	0.0	0.0	0.5788760678616399	0.0
5	0.0	0.0	0.0	0.9493865587483926	0.0
6	1.9107343143043302E-5	0.0	0.0	1.317145592222547	0.0
7	1.9107343143043302E-5	0.0	0.0	1.5087731366041284	0.0
8	3.8214686286086605E-5	0.0	0.0	1.986036353631064	0.0
9	3.8214686286086605E-5	0.0	0.0	2.1164439705823344	0.0
10	3.8214686286086605E-5	0.0	0.0	2.344776721141702	0.0
11	3.8214686286086605E-5	0.0	0.0	2.7231403300602453	0.0
12	3.8214686286086605E-5	0.0	0.0	3.0258388601323376	0.0
13	7.642937257217321E-5	0.0	0.0	3.1449922519723557	0.0
14	9.553671571521652E-5	0.0	0.0	3.1882321695050626	0.0
15	9.553671571521652E-5	0.0	0.0	3.264852615508666	0.0
16	9.553671571521652E-5	0.0	0.0	3.439054262943792	0.0
17	9.553671571521652E-5	0.0	0.0	3.6490057493995516	0.0
18	9.553671571521652E-5	0.0	0.0	3.8946879675328026	0.0
19	9.553671571521652E-5	0.0	0.0	4.04529204618627	0.0
20	9.553671571521652E-5	0.0	0.0	4.1955904073494485	0.0
21	9.553671571521652E-5	0.0	0.0	4.40015362303887	0.0
22	1.1464405885825981E-4	0.0	0.0	4.616276781329833	0.0
23	1.337514020013031E-4	0.0	0.0	4.838743577544286	0.0
24	1.337514020013031E-4	0.0	0.0	5.005416931781053	0.0
25	1.337514020013031E-4	0.0	0.0	5.1538236659730705	0.0
26	1.337514020013031E-4	0.0	0.0	5.289581339004393	0.0
27	1.337514020013031E-4	0.0	0.0	5.420791464367671	0.0
28	1.5285874514434642E-4	0.0	0.0	5.561192221782753	0.0
29	1.5285874514434642E-4	0.0	0.0	5.713191136485663	0.0
30	1.5285874514434642E-4	0.0	0.0	5.9043027826023815	0.0
31	1.5285874514434642E-4	0.0	0.0	6.086625050873301	0.0
32	1.5285874514434642E-4	0.0	0.0	6.249228541020599	0.0
33	1.7196608828738973E-4	0.0	0.0	6.4134943700213425	0.0
34	1.7196608828738973E-4	0.0	0.0	6.590772299702499	0.0
35	1.7196608828738973E-4	0.0	0.0	6.811748723151794	0.0
36	1.7196608828738973E-4	0.0	0.0	7.006911126014839	0.0
37	1.7196608828738973E-4	0.0	0.0	7.1928446821397936	0.0
38	1.7196608828738973E-4	0.0	0.0	7.384357582462516	0.0
39	1.9107343143043304E-4	0.0	0.0	7.592895125525691	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCCG	30	2.1669875E-6	45.000004	31
CGCCTAT	30	2.1669875E-6	45.000004	27
TATCGCC	30	2.1669875E-6	45.000004	31
CGAACCG	35	1.2130295E-7	45.0	29
GTCGATA	40	6.8230293E-9	45.0	12
CTCCGCG	40	6.8230293E-9	45.0	25
ATTACCG	175	0.0	45.0	1
CAATTCG	45	3.8562575E-10	45.0	23
TGACGTA	35	1.2130295E-7	45.0	29
TACGCAC	25	3.8927137E-5	45.0	18
CTCGCTA	45	3.8562575E-10	45.0	38
TACTCGC	160	0.0	45.0	45
TCGGTGA	25	3.8927137E-5	45.0	31
GTTAGCG	400	0.0	43.875	1
CGCTAAG	105	0.0	42.857147	26
ATCGGCC	95	0.0	42.63158	27
GTCGGTA	75	0.0	42.0	15
CTATACG	140	0.0	41.785713	1
ACGTAGA	70	0.0	41.785713	27
CGTTTTT	5065	0.0	41.579468	1
>>END_MODULE