FastQCFastQC Report
Thu 26 May 2016
SRR1545291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545291_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2406996
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30740.12771105560624113No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27070.1124638345888402No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.10141271526832617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGA600.045.00000438
CATACGT302.1664964E-645.00000424
CTCTACA302.1664964E-645.00000420
TCGTTTG406.8212103E-945.01
ACTATCG351.2126657E-745.01
TCGTTGC453.8562575E-1045.030
ATCTCGC207.034827E-445.040
TCGTTAG502.1827873E-1145.01
CGAACTT406.8212103E-945.030
CGAAACG351.2126657E-745.039
CTATCGG351.2126657E-745.02
AACCCGT253.892083E-545.022
CGCATCG351.2126657E-745.021
TAGAACG850.045.01
ACCGAAT253.892083E-545.017
TACGGAC351.2126657E-745.017
TATGCGA351.2126657E-745.034
ATCGTAC406.8212103E-945.040
GTGCTTA406.8212103E-945.030
CTACGTC207.034827E-445.021