##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545291_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2406996
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278417994878264	31.0	31.0	33.0	30.0	34.0
2	31.600684421577768	31.0	31.0	34.0	30.0	34.0
3	31.70626498756126	31.0	31.0	34.0	30.0	34.0
4	30.33542722962564	35.0	33.0	37.0	0.0	37.0
5	32.88735918131979	35.0	33.0	37.0	19.0	37.0
6	34.52245579136816	35.0	33.0	37.0	30.0	37.0
7	35.490483989171565	36.0	35.0	37.0	33.0	37.0
8	35.657397851928295	37.0	35.0	37.0	35.0	37.0
9	37.68748680928427	39.0	37.0	39.0	35.0	39.0
10	37.15557649451848	39.0	37.0	39.0	33.0	39.0
11	36.72101158456433	39.0	35.0	39.0	32.0	39.0
12	35.62466825869258	37.0	35.0	39.0	31.0	39.0
13	35.17496331526932	37.0	35.0	39.0	30.0	39.0
14	36.03147782547208	38.0	35.0	40.0	30.0	41.0
15	36.3524571706808	38.0	35.0	40.0	31.0	41.0
16	36.536280076909144	38.0	35.0	40.0	32.0	41.0
17	36.47061316263093	37.0	35.0	40.0	32.0	41.0
18	36.44311664830353	37.0	35.0	40.0	31.0	41.0
19	36.425766390970324	37.0	35.0	40.0	31.0	41.0
20	36.30721488527609	37.0	35.0	40.0	31.0	41.0
21	36.145277349858496	37.0	34.0	40.0	31.0	41.0
22	36.02919032686386	37.0	34.0	40.0	30.0	41.0
23	35.99429371714785	37.0	34.0	40.0	30.0	41.0
24	35.91627863112361	36.0	34.0	40.0	30.0	41.0
25	35.80308234828807	36.0	34.0	40.0	30.0	41.0
26	35.61551535607039	36.0	34.0	40.0	30.0	41.0
27	35.61101181721947	36.0	34.0	40.0	30.0	41.0
28	35.608263578335816	36.0	34.0	40.0	30.0	41.0
29	35.65991717476888	36.0	34.0	40.0	30.0	41.0
30	35.66265045725045	36.0	34.0	40.0	30.0	41.0
31	35.561155066315024	36.0	34.0	40.0	30.0	41.0
32	35.461456105452605	36.0	34.0	40.0	30.0	41.0
33	35.33648996508511	36.0	34.0	40.0	29.0	41.0
34	35.25541712574512	36.0	34.0	40.0	29.0	41.0
35	35.13202473124176	36.0	34.0	40.0	29.0	41.0
36	34.945175230868685	35.0	34.0	40.0	27.0	41.0
37	34.900737682987426	35.0	33.0	40.0	27.0	41.0
38	34.891966168618474	35.0	33.0	40.0	27.0	41.0
39	34.84546546816031	35.0	34.0	40.0	27.0	41.0
40	34.671232108404006	35.0	33.0	40.0	27.0	41.0
41	34.646656662495495	35.0	34.0	40.0	27.0	41.0
42	34.58886720210586	35.0	33.0	40.0	27.0	41.0
43	34.54013342772485	35.0	33.0	40.0	27.0	41.0
44	34.494932687881494	35.0	33.0	40.0	27.0	41.0
45	34.40492963012817	35.0	33.0	39.0	27.0	41.0
46	34.35369065839744	35.0	33.0	39.0	27.0	41.0
47	34.29885342559771	35.0	33.0	39.0	27.0	41.0
48	34.19837465454866	35.0	33.0	39.0	26.0	41.0
49	34.18098783712146	35.0	33.0	39.0	26.0	41.0
50	34.01377733905665	35.0	33.0	39.0	26.0	40.0
51	33.939671274900334	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	4.0
13	11.0
14	21.0
15	57.0
16	134.0
17	435.0
18	926.0
19	1881.0
20	3409.0
21	5482.0
22	8591.0
23	12621.0
24	18027.0
25	24995.0
26	32869.0
27	40841.0
28	49090.0
29	60390.0
30	75134.0
31	94917.0
32	121229.0
33	177844.0
34	270017.0
35	363741.0
36	177208.0
37	223702.0
38	289293.0
39	353937.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.69332229883224	25.443291139661223	30.650736436620583	12.212650124885958
2	28.137354611308034	27.009226438265788	31.277742048595016	13.575676901831162
3	29.246538008372262	26.42605139352122	30.28883305165443	14.03857754645209
4	23.126544456243384	38.37829393983206	25.608143927119116	12.887017676805446
5	24.00934608948249	33.05813553491572	29.00744330277242	13.925075072829369
6	22.771122178848657	41.64680788833883	24.46917236256313	11.11289757024939
7	90.11232257968024	4.136691544148807	4.007318666088352	1.7436672100826092
8	91.54464735296611	3.0657300635314724	3.5174549521478222	1.8721676313546012
9	88.16686857809485	4.631457634329263	4.6868378676159	2.514835919959983
10	59.475586997236384	22.22525504820116	9.155519992555035	9.143637962007416
11	52.478442008212724	19.943780546373986	14.861013479041926	12.716763966371362
12	48.52816539786522	20.695298205730296	17.596497875359994	13.180038521044487
13	23.50735107162621	40.48569253958046	18.57730548783629	17.429650900957043
14	14.936709491831312	43.117022213580746	23.13057437569485	18.815693918893093
15	15.257108861003507	23.93946645528285	46.398913832843924	14.404510850869714
16	17.47410465160723	17.788770733312393	45.7504291656488	18.986695449431572
17	17.523377687374637	19.593094462973763	25.859536118880133	37.02399173077146
18	23.241127114461346	25.96028410516677	28.563612070813576	22.234976709558303
19	33.873633358759214	26.07951986625653	20.050344911250374	19.996501863733883
20	37.251869134805375	23.922682048495304	20.537258890334677	18.288189926364648
21	26.455798015451627	28.162406584805293	22.714038577546454	22.66775682219663
22	24.220605268974275	27.29256716670904	21.451261240151627	27.03556632416506
23	24.47137427731496	30.61928644667461	21.917028528506073	22.99231074750436
24	23.940754367684868	23.02999257165363	34.401054260164955	18.628198800496556
25	21.415698239631475	24.39979127509975	31.253105530711313	22.931404954557465
26	19.797747898210048	36.284189919717356	22.722887782115134	21.195174399957455
27	22.630531999222267	33.497022845073275	26.210263747841704	17.662181407862747
28	18.09824361984814	29.935031051152556	34.99764852122729	16.96907680777201
29	19.161394534930675	26.2559223197712	32.81945628492943	21.763226860368693
30	22.89152121565636	29.858961128310973	25.882012267573355	21.367505388459307
31	30.668144026828465	27.914379583514055	19.79164070069082	21.625835688966664
32	33.62452617287274	27.804283845922466	21.120849390692797	17.45034059051199
33	29.942758525564646	28.381808694322714	20.930903084176293	20.744529695936347
34	21.057118499573743	27.511096819438002	26.45583956101298	24.975945119975272
35	23.58529054472878	24.1461971685869	27.642463884443515	24.6260484022408
36	30.79942800071126	26.82817088187932	22.129783348206644	20.242617769202774
37	23.778975951767265	33.37325861779579	24.55961704963365	18.288148380803293
38	23.119980257549244	32.607573922017316	20.598372411088345	23.67407340934509
39	24.97295383955769	30.589664461428267	23.279598304276366	21.157783394737674
40	26.51338016349009	25.138305173751846	23.05845128118202	25.28986338157604
41	17.089226571211587	24.93394255744505	26.478855802003824	31.49797506933954
42	23.25421396628827	26.605071217401278	22.004731208527144	28.13598360778331
43	23.78221650555298	27.92784034539318	22.83044924046405	25.459493908589792
44	22.354918745191103	30.86789508582482	24.768632768812246	22.00855340017183
45	19.9763107209152	34.580364902974495	20.041578797804398	25.401745578305906
46	21.613081201630578	33.10508201924723	22.424258287093124	22.857578492029067
47	23.510840898780057	25.91686899355047	26.50365850213295	24.068631605536527
48	24.85596153878112	20.88092377386585	30.025600374907146	24.23751431244589
49	21.144114905051776	24.86385519543863	28.983222240502272	25.008807659007324
50	19.218353499548815	32.65456195191018	24.335686473928497	23.791398074612506
51	18.222464848300536	33.79116541946892	21.77922190149049	26.20714783074006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	326.0
1	831.5
2	1337.0
3	3424.0
4	5511.0
5	4213.5
6	2916.0
7	3145.0
8	3374.0
9	3652.0
10	3930.0
11	3989.5
12	4049.0
13	4003.5
14	3958.0
15	3935.5
16	3913.0
17	3995.0
18	4077.0
19	4066.5
20	4056.0
21	4495.0
22	4934.0
23	5362.0
24	5790.0
25	7510.5
26	12573.5
27	15916.0
28	21457.5
29	26999.0
30	30053.0
31	33107.0
32	35763.0
33	38419.0
34	43670.5
35	48922.0
36	49550.5
37	50179.0
38	68792.5
39	87406.0
40	124612.5
41	161819.0
42	196124.5
43	230430.0
44	229440.0
45	228450.0
46	211637.5
47	194825.0
48	182393.0
49	169961.0
50	162526.5
51	155092.0
52	144989.5
53	134887.0
54	128709.0
55	122531.0
56	126879.0
57	131227.0
58	133690.5
59	136154.0
60	132010.0
61	127866.0
62	110587.5
63	93309.0
64	75462.0
65	57615.0
66	48674.5
67	39734.0
68	33029.0
69	26324.0
70	21541.5
71	16759.0
72	13145.0
73	9531.0
74	7740.5
75	4761.5
76	3573.0
77	2565.5
78	1558.0
79	1047.0
80	536.0
81	390.5
82	245.0
83	201.5
84	158.0
85	125.5
86	93.0
87	52.0
88	11.0
89	6.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2406996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.976756134808625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82118746579158	11.804857029311947
2	8.755695921691332	2.6226384521941903
3	2.9145507810858198	1.3095154887251497
4	1.4671551795034103	0.878929013413758
5	0.7956537311092535	0.5958155949286942
6	0.5041060925549095	0.45299244085596874
7	0.3430032123345186	0.3595952825213046
8	0.2515391137310493	0.30137919717726536
9	0.21854839377960406	0.2945831397562131
>10	2.46630998641895	9.267142690678916
>50	1.3482163377437328	14.84543176338486
>100	2.098268725202181	55.233627312871455
>500	0.013642839565669971	1.3514087324012192
>1k	0.0021222194879931067	0.682083861779092
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3074	0.12771105560624113	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2707	0.1124638345888402	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2441	0.10141271526832617	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.061695158612644144	0.0
2	0.0	0.0	0.0	0.181346375315954	0.0
3	0.0	0.0	0.0	0.26011675964563297	0.0
4	0.0	0.0	0.0	0.4385549456667149	0.0
5	0.0	0.0	0.0	0.7254270468251713	0.0
6	0.0	0.0	0.0	1.0186971644323464	0.0
7	0.0	0.0	0.0	1.1722080136402386	0.0
8	0.0	0.0	0.0	1.5378089535670187	0.0
9	0.0	0.0	0.0	1.640135671185162	0.0
10	0.0	0.0	0.0	1.8147101200001994	0.0
11	0.0	0.0	0.0	2.0882460959635996	0.0
12	0.0	0.0	0.0	2.300710096734685	0.0
13	0.0	0.0	0.0	2.390365418139457	0.0
14	0.0	0.0	0.0	2.42626078315045	0.0
15	0.0	0.0	0.0	2.488329851815292	0.0
16	0.0	0.0	0.0	2.62094328366146	0.0
17	0.0	0.0	0.0	2.7728338559764953	0.0
18	0.0	0.0	0.0	2.940220922677063	0.0
19	0.0	0.0	0.0	3.0434200970836676	0.0
20	0.0	0.0	0.0	3.1515216477302	0.0
21	0.0	0.0	0.0	3.3083561418465175	0.0
22	0.0	0.0	0.0	3.4772388487558765	0.0
23	0.0	0.0	0.0	3.642548637388679	0.0
24	0.0	0.0	0.0	3.771630696519645	0.0
25	0.0	0.0	0.0	3.8771564223621477	0.0
26	0.0	0.0	0.0	3.9781121364555654	0.0
27	0.0	0.0	0.0	4.073542290888726	0.0
28	0.0	0.0	0.0	4.181062203676284	0.0
29	4.154556135531592E-5	0.0	0.0	4.291905761372266	0.0
30	4.154556135531592E-5	0.0	0.0	4.430335571808179	0.0
31	4.154556135531592E-5	0.0	0.0	4.5817275973869505	0.0
32	4.154556135531592E-5	0.0	0.0	4.718038584193742	0.0
33	4.154556135531592E-5	0.0	0.0	4.8447110007661	0.0
34	4.154556135531592E-5	0.0	0.0	4.982683810027105	0.0
35	4.154556135531592E-5	0.0	0.0	5.146996505187379	0.0
36	4.154556135531592E-5	0.0	0.0	5.31878740139161	0.0
37	4.154556135531592E-5	0.0	0.0	5.487960927230456	0.0
38	4.154556135531592E-5	0.0	0.0	5.630379111556479	0.0
39	4.154556135531592E-5	0.0	0.0	5.788667700320233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCGA	60	0.0	45.000004	38
CATACGT	30	2.1664964E-6	45.000004	24
CTCTACA	30	2.1664964E-6	45.000004	20
TCGTTTG	40	6.8212103E-9	45.0	1
ACTATCG	35	1.2126657E-7	45.0	1
TCGTTGC	45	3.8562575E-10	45.0	30
ATCTCGC	20	7.034827E-4	45.0	40
TCGTTAG	50	2.1827873E-11	45.0	1
CGAACTT	40	6.8212103E-9	45.0	30
CGAAACG	35	1.2126657E-7	45.0	39
CTATCGG	35	1.2126657E-7	45.0	2
AACCCGT	25	3.892083E-5	45.0	22
CGCATCG	35	1.2126657E-7	45.0	21
TAGAACG	85	0.0	45.0	1
ACCGAAT	25	3.892083E-5	45.0	17
TACGGAC	35	1.2126657E-7	45.0	17
TATGCGA	35	1.2126657E-7	45.0	34
ATCGTAC	40	6.8212103E-9	45.0	40
GTGCTTA	40	6.8212103E-9	45.0	30
CTACGTC	20	7.034827E-4	45.0	21
>>END_MODULE