##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545290_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3314430
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358510513119903	31.0	31.0	33.0	30.0	34.0
2	31.672558479135176	31.0	31.0	34.0	30.0	34.0
3	31.753495171115397	31.0	31.0	34.0	30.0	34.0
4	30.294169736576123	35.0	33.0	37.0	0.0	37.0
5	32.89682750880242	35.0	33.0	37.0	19.0	37.0
6	34.55118708194169	35.0	33.0	37.0	30.0	37.0
7	35.54970145696243	37.0	35.0	37.0	33.0	37.0
8	35.75540711374203	37.0	35.0	37.0	35.0	37.0
9	37.80195207019005	39.0	37.0	39.0	35.0	39.0
10	37.24083688598039	39.0	37.0	39.0	34.0	39.0
11	36.79817223474323	39.0	35.0	39.0	33.0	39.0
12	35.63718135546685	37.0	35.0	39.0	31.0	39.0
13	35.21055173891136	37.0	35.0	39.0	30.0	39.0
14	36.01136907401876	38.0	35.0	40.0	31.0	41.0
15	36.38011573634079	38.0	35.0	40.0	31.0	41.0
16	36.56503591869492	37.0	35.0	40.0	32.0	41.0
17	36.50948519051541	37.0	35.0	40.0	32.0	41.0
18	36.47450692879318	37.0	35.0	40.0	31.0	41.0
19	36.4774492144954	37.0	35.0	40.0	31.0	41.0
20	36.38304384162586	37.0	35.0	40.0	31.0	41.0
21	36.221980249997735	37.0	35.0	40.0	31.0	41.0
22	36.09029968953938	37.0	35.0	40.0	31.0	41.0
23	36.05513859094928	36.0	34.0	40.0	31.0	41.0
24	36.007844486080565	36.0	34.0	40.0	31.0	41.0
25	35.89598513168177	36.0	34.0	40.0	31.0	41.0
26	35.75976140693875	36.0	34.0	40.0	30.0	41.0
27	35.74273283792387	36.0	34.0	40.0	30.0	41.0
28	35.80644515044819	36.0	34.0	40.0	30.0	41.0
29	35.87486415462085	36.0	35.0	40.0	30.0	41.0
30	35.84917647981704	36.0	35.0	40.0	30.0	41.0
31	35.69515904695528	36.0	34.0	40.0	30.0	41.0
32	35.58519383423394	36.0	34.0	40.0	30.0	41.0
33	35.45538780423783	36.0	34.0	40.0	30.0	41.0
34	35.408609021762416	36.0	34.0	40.0	30.0	41.0
35	35.2642351776927	36.0	34.0	40.0	29.0	41.0
36	35.09657859722486	36.0	34.0	40.0	28.0	41.0
37	35.02810528507164	35.0	34.0	40.0	28.0	41.0
38	35.039457463274225	35.0	34.0	40.0	29.0	41.0
39	34.97329917964778	35.0	34.0	40.0	28.0	41.0
40	34.862127122914046	35.0	34.0	40.0	27.0	41.0
41	34.899389035218725	35.0	34.0	40.0	28.0	41.0
42	34.860882565026266	35.0	34.0	40.0	28.0	41.0
43	34.78745425306916	35.0	33.0	40.0	27.0	41.0
44	34.725536215880254	35.0	34.0	40.0	27.0	41.0
45	34.621007232012744	35.0	33.0	40.0	27.0	41.0
46	34.59693431449752	35.0	34.0	40.0	27.0	41.0
47	34.568445253029935	35.0	33.0	40.0	27.0	41.0
48	34.50933403330286	35.0	33.0	39.0	27.0	41.0
49	34.50086500544588	35.0	34.0	39.0	27.0	41.0
50	34.321769655717574	35.0	33.0	39.0	27.0	41.0
51	34.23629160971871	35.0	33.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	6.0
14	10.0
15	40.0
16	157.0
17	453.0
18	1060.0
19	2202.0
20	4072.0
21	6623.0
22	10458.0
23	15749.0
24	22626.0
25	31713.0
26	41776.0
27	51733.0
28	63037.0
29	78193.0
30	97723.0
31	125502.0
32	163939.0
33	245399.0
34	377281.0
35	521848.0
36	232296.0
37	294408.0
38	401139.0
39	524699.0
40	281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.60495168098285	25.236828051882227	31.0725222738148	12.08569799332012
2	27.731676336504318	26.428164118717245	32.8600091116723	12.980150433106145
3	28.292466577963634	26.517108522430703	31.6308083139484	13.559616585657263
4	22.0614705997713	39.0627649399746	26.199980087073794	12.675784373180305
5	23.50696801561656	32.38541770379824	29.179949493578082	14.927664787007117
6	22.10193004528682	40.573643130191314	25.917005337267646	11.407421487254219
7	90.32675301635575	4.199243912226235	3.955370908421659	1.5186321629963524
8	93.05880649161395	2.1317089212926508	3.109041373629855	1.7004432134635519
9	89.59483229393894	4.057500082970526	4.414273344134587	1.933394278955959
10	57.653865068805196	25.616953744686118	8.396345676330471	8.33283551017822
11	51.25481606188696	18.163243755336513	18.85506708544154	11.726873097334986
12	44.710825089080174	22.99565837866541	19.284944922656386	13.008571609598029
13	20.17297091807641	44.91722558629991	20.94203226497467	13.96777123064901
14	13.01288607694234	45.84103450668742	26.469196815138652	14.676882601231583
15	12.784460676496412	23.60040791327619	50.335442293244995	13.279689116982407
16	15.614449543360397	19.071424045763525	46.529116620354024	18.78500979052205
17	16.33608192057156	19.160157251774816	26.611544066400555	37.89221676125307
18	22.25405273304912	23.588792039656894	33.316256490557954	20.84089873673603
19	31.605947327293077	24.42021705089563	23.514631475095264	20.459204146716026
20	33.80071384823333	24.827858787182112	22.350268371937258	19.0211589926473
21	22.873314566908938	29.422042402464378	25.77927426435315	21.925368766273536
22	22.728342429920076	25.85424944862314	23.655500342441986	27.761907779014795
23	20.562811705180074	32.33044595903368	24.093795916643284	23.01294641914296
24	20.025223039859043	24.09488207625444	38.7383350983427	17.14155978554382
25	16.760438446429703	26.362753173245473	35.59224361353234	21.284564766792478
26	17.197014267913335	38.36008001375802	24.708351058854767	19.734554659473876
27	17.807767851485774	35.80196293178616	28.626973567099018	17.763295649629047
28	14.520747157128072	29.085755318410705	39.643437936538106	16.75005958792311
29	14.571193236846156	25.274149702965516	39.10515533590995	21.049501724278382
30	19.669415253904894	31.07333689352317	30.153088162972214	19.10415968959972
31	30.81078797862679	26.516836982527913	22.948561291081724	19.723813747763565
32	33.01973491671268	26.223272176513007	24.137393156591028	16.61959975018329
33	29.405538810594884	26.460477367149103	24.111717550227336	20.022266272028673
34	19.205353560038983	27.632413416484887	27.706815349848995	25.45541767362714
35	20.55321729528154	25.52683266806057	31.144721716856296	22.775228319801595
36	31.67144878606578	24.10722205628117	25.32175969925447	18.89956945839858
37	20.729688060993897	33.067013030898224	27.859088893112844	18.34421001499504
38	21.12176150952049	34.31214416958572	22.729126878528135	21.83696744236566
39	21.353807442003603	33.059168544817666	25.567925706682598	20.01909830649614
40	25.38620516951633	25.787692001339597	23.501446704259855	25.32465612488422
41	17.35106790609547	24.224255754383105	26.031172780840144	32.39350355868128
42	22.089982289564116	26.30847536378804	22.997347960282763	28.60419438636508
43	22.380680841049593	26.933982615412	25.470593737082996	25.21474280645541
44	19.90891344816454	31.587723982705924	28.163153242035584	20.34020932709395
45	17.67646322293728	37.901388775747265	21.51869250519697	22.90345549611849
46	21.102542518623114	33.74480679935917	25.262292460543744	19.890358221473978
47	22.217183648470478	25.46543447893001	27.455791795270983	24.86159007732853
48	23.204502735010244	21.660194965650202	32.068228926240714	23.067073373098843
49	20.525459882996472	23.256789251847227	32.59568010185763	23.622070763298666
50	19.10853449914465	34.900691823330106	25.157357373666063	20.833416303859185
51	16.962132252001098	36.03096158313798	23.677766614470663	23.329139550390263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	379.0
1	770.0
2	1161.0
3	3887.5
4	6614.0
5	5112.0
6	3610.0
7	4035.5
8	4461.0
9	4963.0
10	5465.0
11	5968.0
12	6471.0
13	6328.5
14	6186.0
15	6074.0
16	5962.0
17	5957.0
18	5952.0
19	6638.0
20	7324.0
21	9599.5
22	11875.0
23	11419.5
24	10964.0
25	14505.0
26	22147.0
27	26248.0
28	34647.5
29	43047.0
30	49104.5
31	55162.0
32	61855.0
33	68548.0
34	77814.0
35	87080.0
36	93933.5
37	100787.0
38	133123.0
39	165459.0
40	221775.0
41	278091.0
42	329753.0
43	381415.0
44	376282.5
45	371150.0
46	341484.0
47	311818.0
48	289731.5
49	267645.0
50	253654.0
51	239663.0
52	211598.0
53	183533.0
54	167295.0
55	151057.0
56	132505.5
57	113954.0
58	108249.5
59	102545.0
60	97960.5
61	93376.0
62	77888.0
63	62400.0
64	52375.0
65	42350.0
66	32753.5
67	23157.0
68	21521.5
69	19886.0
70	15878.5
71	11871.0
72	10182.0
73	8493.0
74	7358.0
75	4617.5
76	3012.0
77	1983.0
78	954.0
79	715.5
80	477.0
81	371.0
82	265.0
83	255.0
84	245.0
85	140.0
86	35.0
87	23.0
88	11.0
89	6.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3314430.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.769294077315442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37968874122551	10.654636898890688
2	10.153113015431673	2.7960239781939533
3	3.5459480633822182	1.464756050027908
4	1.7344805222241584	0.9553028952752041
5	1.001298735775444	0.689358837606813
6	0.5947810655315341	0.4913829241753632
7	0.4005202553840521	0.38604168262131483
8	0.28000677537832774	0.3084396507060003
9	0.21162056038732893	0.26224791559014815
>10	1.802832931796562	5.642777928418056
>50	0.7602094502785678	7.712796666763294
>100	2.0916148210672993	64.49446769609696
>500	0.04052773497924665	3.3846508869597995
>1k	0.0033573271580441015	0.7571159886744899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3838	0.11579668298923194	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.061217162528700256	0.0
2	0.0	0.0	0.0	0.18745304622514278	0.0
3	0.0	0.0	0.0	0.26776851524998263	0.0
4	0.0	0.0	0.0	0.46336775855878687	0.0
5	0.0	0.0	0.0	0.7631176401372182	0.0
6	0.0	0.0	0.0	1.0804572731962963	0.0
7	0.0	0.0	0.0	1.2532169935705386	0.0
8	0.0	0.0	0.0	1.6920858186777215	0.0
9	3.01711003098572E-5	0.0	0.0	1.816632120756812	0.0
10	6.03422006197144E-5	0.0	0.0	2.0273772564211643	0.0
11	6.03422006197144E-5	0.0	0.0	2.3309286966386376	0.0
12	6.03422006197144E-5	0.0	0.0	2.5885597221845082	0.0
13	6.03422006197144E-5	0.0	0.0	2.688546748611375	0.0
14	6.03422006197144E-5	0.0	0.0	2.725053779986302	0.0
15	6.03422006197144E-5	0.0	0.0	2.7820771595719322	0.0
16	6.03422006197144E-5	0.0	0.0	2.9181186508690784	0.0
17	6.03422006197144E-5	0.0	0.0	3.0801072884327017	0.0
18	6.03422006197144E-5	0.0	0.0	3.2840940976276465	0.0
19	6.03422006197144E-5	0.0	0.0	3.4091834795123144	0.0
20	6.03422006197144E-5	0.0	0.0	3.546130103818756	0.0
21	6.03422006197144E-5	0.0	0.0	3.7108341404102667	0.0
22	6.03422006197144E-5	0.0	0.0	3.890171160652058	0.0
23	6.03422006197144E-5	0.0	0.0	4.081667134318722	0.0
24	6.03422006197144E-5	0.0	0.0	4.21921718063136	0.0
25	6.03422006197144E-5	0.0	0.0	4.340716201579156	0.0
26	6.03422006197144E-5	0.0	0.0	4.450448493406106	0.0
27	6.03422006197144E-5	0.0	0.0	4.555926660089367	0.0
28	6.03422006197144E-5	0.0	0.0	4.666594256025923	0.0
29	6.03422006197144E-5	0.0	0.0	4.797084264866055	0.0
30	6.03422006197144E-5	0.0	0.0	4.965469175695368	0.0
31	6.03422006197144E-5	0.0	0.0	5.12996201458471	0.0
32	6.03422006197144E-5	0.0	0.0	5.266154361383405	0.0
33	6.03422006197144E-5	0.0	0.0	5.400174388959791	0.0
34	6.03422006197144E-5	0.0	0.0	5.553534091834795	0.0
35	6.03422006197144E-5	0.0	0.0	5.747986833331825	0.0
36	6.03422006197144E-5	0.0	0.0	5.907652296171589	0.0
37	6.03422006197144E-5	0.0	0.0	6.074920876289437	0.0
38	6.03422006197144E-5	0.0	0.0	6.222668754506808	0.0
39	6.03422006197144E-5	0.0	0.0	6.396756003294684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	30	2.1667438E-6	45.000004	41
GGTACGA	35	1.2128476E-7	45.000004	9
ACACGGC	25	3.8924016E-5	45.000004	38
GATCGAC	20	7.0352113E-4	45.000004	9
GGTCGAC	60	0.0	45.000004	9
GCGGTAC	25	3.8924016E-5	45.000004	31
TAGACCG	60	0.0	45.000004	1
ACGCACC	20	7.0352113E-4	45.000004	41
CCGGCTA	20	7.0352113E-4	45.000004	20
CGGTTTA	35	1.2128476E-7	45.000004	36
ATTACGA	30	2.1667438E-6	45.000004	21
GCTATCG	40	6.8212103E-9	45.000004	37
TATATCG	20	7.0352113E-4	45.000004	1
CGTCTAC	20	7.0352113E-4	45.000004	38
CGCGATA	20	7.0352113E-4	45.000004	11
CTACGTC	35	1.2128476E-7	45.000004	38
ACTACGC	20	7.0352113E-4	45.000004	38
CGTACTA	20	7.0352113E-4	45.000004	21
CGTACAG	50	2.1827873E-11	45.000004	20
ACCACGT	20	7.0352113E-4	45.000004	26
>>END_MODULE