FastQCFastQC Report
Thu 26 May 2016
SRR1545289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545289_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3256446
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38730.11893334021199797No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA207.035194E-445.029
CGTCGTT253.892387E-545.029
ATACGAC253.892387E-545.012
ACGCGTC406.8212103E-945.035
TAGTACG850.042.3529431
TATTACG700.041.7857131
TACGATC650.041.53846411
TCGAACG603.6379788E-1241.2500041
CGTATTT6200.041.0080641
CGCATAT556.184564E-1140.90909229
TACGGGA8500.040.7647064
ACGTTAG1550.040.645161
TGTTACG1450.040.344831
ACGGGAT10300.039.7572825
CTATACG800.039.3751
TATAGCG2000.039.3751
TACGTCA403.4608274E-739.37538
TAGACGC356.251821E-638.57142645
CCGTCGA356.251821E-638.57142619
CCGAATA356.251821E-638.57142638
Produced by FastQC (version 0.11.5)