##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545289_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3256446
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266460429560325	31.0	31.0	33.0	30.0	34.0
2	31.595255379637802	31.0	31.0	34.0	30.0	34.0
3	31.69722759106093	31.0	31.0	34.0	30.0	34.0
4	30.330658638282348	35.0	33.0	37.0	0.0	37.0
5	32.88925012114434	35.0	33.0	37.0	19.0	37.0
6	34.51128162420012	35.0	33.0	37.0	30.0	37.0
7	35.48892197199032	36.0	35.0	37.0	33.0	37.0
8	35.654591846448554	37.0	35.0	37.0	35.0	37.0
9	37.67425131569816	39.0	37.0	39.0	35.0	39.0
10	37.13620278057736	39.0	37.0	39.0	33.0	39.0
11	36.74020143432441	39.0	35.0	39.0	32.0	39.0
12	35.80491922789446	37.0	35.0	39.0	31.0	39.0
13	35.42173215831001	37.0	35.0	39.0	30.0	39.0
14	36.212717176946896	38.0	35.0	40.0	30.0	41.0
15	36.50430254332484	38.0	35.0	40.0	31.0	41.0
16	36.65925582675101	38.0	35.0	40.0	32.0	41.0
17	36.60719661864499	38.0	35.0	40.0	31.0	41.0
18	36.56305217405724	38.0	35.0	40.0	31.0	41.0
19	36.55846680706512	38.0	35.0	40.0	31.0	41.0
20	36.478866838264786	38.0	35.0	40.0	31.0	41.0
21	36.32319344463259	38.0	35.0	40.0	31.0	41.0
22	36.2394850091173	38.0	35.0	40.0	30.0	41.0
23	36.16254591662199	37.0	34.0	40.0	30.0	41.0
24	36.09232181341254	37.0	34.0	40.0	30.0	41.0
25	35.9581249005818	37.0	34.0	40.0	30.0	41.0
26	35.831713776307055	37.0	34.0	40.0	30.0	41.0
27	35.84115136562989	37.0	34.0	40.0	30.0	41.0
28	35.85624297163226	37.0	34.0	40.0	30.0	41.0
29	35.879365111535705	37.0	34.0	40.0	30.0	41.0
30	35.80463548297745	37.0	34.0	40.0	30.0	41.0
31	35.686619400413825	37.0	34.0	40.0	30.0	41.0
32	35.608040483398156	37.0	34.0	40.0	30.0	41.0
33	35.47860243959212	37.0	34.0	40.0	29.0	41.0
34	35.357925480723466	37.0	34.0	40.0	29.0	41.0
35	35.217855293777326	36.0	34.0	40.0	28.0	41.0
36	35.05105443173325	36.0	34.0	40.0	27.0	41.0
37	35.00104162636199	36.0	33.0	40.0	27.0	41.0
38	34.93612330743393	36.0	33.0	40.0	27.0	41.0
39	34.868369688918534	36.0	33.0	40.0	27.0	41.0
40	34.745683791470825	36.0	33.0	40.0	26.0	41.0
41	34.720804828331254	36.0	33.0	40.0	26.0	41.0
42	34.660515175132645	35.0	33.0	40.0	27.0	41.0
43	34.58254704668832	35.0	33.0	40.0	27.0	41.0
44	34.507634089433694	35.0	33.0	40.0	27.0	41.0
45	34.40782435821138	35.0	33.0	40.0	26.0	41.0
46	34.375999172103576	35.0	33.0	39.0	26.0	41.0
47	34.29090794074276	35.0	33.0	39.0	26.0	41.0
48	34.17706788320764	35.0	33.0	39.0	26.0	41.0
49	34.102150319704364	35.0	33.0	39.0	26.0	41.0
50	33.94733982998643	35.0	33.0	39.0	25.0	41.0
51	33.85630776619664	35.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	4.0
14	32.0
15	76.0
16	204.0
17	596.0
18	1341.0
19	2569.0
20	4752.0
21	7754.0
22	12057.0
23	17370.0
24	24652.0
25	33765.0
26	43795.0
27	54733.0
28	66556.0
29	81845.0
30	100871.0
31	126995.0
32	161749.0
33	231385.0
34	338404.0
35	439990.0
36	262188.0
37	331364.0
38	421847.0
39	489321.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89472633662588	25.05737850405012	30.212507746174815	11.835387413149181
2	29.419066061589845	26.194415629800094	31.11044985852675	13.276068450083311
3	29.75937571204927	26.513628661430282	30.139606184165192	13.587389442355255
4	22.552377653429538	38.46656754019566	25.42044302285375	13.560611783521054
5	24.101704741918027	32.18223179503054	28.653998868705333	15.062064594346106
6	22.513316664854877	41.13281780198413	25.409050234519476	10.944815298641524
7	89.47383128723769	4.2893387453684175	4.368074889004761	1.8687550783891398
8	91.0707869867948	2.753124111377864	4.079232390157859	2.09685651166947
9	87.2281315274382	4.57550961999677	5.214488433095467	2.9818704194695687
10	55.92056493490142	23.306082766304122	10.092935672816315	10.680416625978136
11	46.66464605892436	20.553020071574963	17.547565659003713	15.234768210496966
12	40.712973591455224	21.379350371540013	22.00518602181642	15.902490015188336
13	23.660149746072868	35.363460656187755	23.274637442168547	17.701752155570826
14	17.196047470156113	37.3165100849208	27.716596559562173	17.770845885360913
15	16.971231827581356	22.440046602953036	42.879445874428754	17.709275695036858
16	21.089248831394716	19.34516954987124	39.934701819099715	19.630879799634325
17	21.843967318972894	19.55149878118661	25.399070029105346	33.20546387073515
18	24.88943467817369	24.24471340842133	29.33464887794854	21.531203035456446
19	31.62975833162902	24.385449658922642	22.830103738861325	21.154688270587013
20	33.88242888105622	24.124858818478796	22.749647929061314	19.24306437140367
21	26.22629087047659	26.729170390050992	26.07747833067092	20.967060408801498
22	26.315345011094916	23.71210823087501	24.098449659536808	25.874097098493266
23	24.521978868987848	27.885123843601278	24.01295768454321	23.579939602867668
24	23.749695219880813	23.304455225113514	33.18246333579614	19.76338621920953
25	22.135143650470482	24.924104376366138	30.606280589329593	22.334471383833787
26	20.420022318810137	32.75110350363556	24.67367799128252	22.155196186271784
27	20.76549096776056	30.575295890059284	27.701211689062248	20.95800145311791
28	18.813454913731103	27.477655087785884	33.393091732520666	20.31579826596234
29	19.602720266204322	24.987946982692176	32.7335383421067	22.6757944089968
30	22.75833838485269	27.738184511581032	28.179033215966115	21.324443887600165
31	30.03341679855892	25.09917253349203	23.413838276452303	21.453572391496742
32	32.76891433175923	24.775383961533525	23.591363099526294	18.864338607180958
33	30.064984955991903	26.34863283469156	22.52360395351251	21.062778255804027
34	22.294335603906838	26.45359388732379	26.30610180546522	24.945968703304157
35	23.319471595721225	25.610865342155222	28.581926431453187	22.48773663067037
36	31.893481421156682	24.442536433891426	24.05945008761085	19.60453205734104
37	23.790168791375628	30.687688357184488	25.29524518447412	20.226897666965765
38	23.761763591350814	31.080632075581782	22.16265830908911	22.994946023978287
39	23.891966886599686	29.78618407920782	24.417631982842643	21.904217051349846
40	26.51731366035242	24.936633372701404	24.11530852960559	24.430744437340586
41	20.54948861427458	23.717297937690354	25.95154349250686	29.781669955528205
42	24.11702819576925	24.829154237472387	23.669669326621722	27.38414824013664
43	23.3335052999497	24.8882984701727	25.808289159408755	25.96990707046885
44	22.06583496240994	27.711498977719884	27.446271180299014	22.776394879571164
45	20.611120221247337	32.304205259353296	22.45325118242403	24.63142333697534
46	23.67633303300592	29.86538084770943	25.076417665147833	21.38186845413681
47	22.695631986527644	25.99736031243878	26.630873043802968	24.676134657230612
48	24.191219507401627	23.107184949481734	29.45373575978229	23.247859783334345
49	23.39716365632963	23.23511582872862	29.668294822023768	23.699425692917984
50	21.26207528084298	30.257372608051845	25.297118392259538	23.183433718845635
51	20.035431264636355	31.772398498240108	23.712998772281193	24.47917146484235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	1145.0
2	1826.0
3	5225.0
4	8624.0
5	6370.0
6	4116.0
7	4361.0
8	4606.0
9	4926.5
10	5247.0
11	5271.0
12	5295.0
13	5587.0
14	5879.0
15	5634.5
16	5390.0
17	5372.0
18	5354.0
19	6111.0
20	6868.0
21	7406.0
22	7944.0
23	8229.0
24	8514.0
25	11120.0
26	16962.0
27	20198.0
28	24620.0
29	29042.0
30	38466.5
31	47891.0
32	50494.0
33	53097.0
34	62571.0
35	72045.0
36	76367.5
37	80690.0
38	100170.0
39	119650.0
40	161087.0
41	202524.0
42	240182.0
43	277840.0
44	282977.0
45	288114.0
46	265560.5
47	243007.0
48	244571.5
49	246136.0
50	239004.5
51	231873.0
52	212181.0
53	192489.0
54	188134.0
55	183779.0
56	177896.5
57	172014.0
58	170609.5
59	169205.0
60	161984.0
61	154763.0
62	136730.5
63	118698.0
64	103923.5
65	89149.0
66	77225.0
67	65301.0
68	54149.0
69	42997.0
70	36930.5
71	30864.0
72	24704.5
73	18545.0
74	14732.0
75	8567.0
76	6215.0
77	4704.0
78	3193.0
79	2324.5
80	1456.0
81	991.0
82	526.0
83	352.5
84	179.0
85	174.5
86	170.0
87	92.0
88	14.0
89	11.5
90	9.0
91	5.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3256446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.017669459609994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87118617130432	12.473149205197055
2	9.098178743130504	2.9146323958382867
3	3.1250017671541395	1.501657361009164
4	1.5232193435940038	0.9759369584069144
5	0.8967616103685717	0.7182015529475676
6	0.573893323309837	0.5515460134712438
7	0.40865540145968465	0.45819950004258175
8	0.2878402962130727	0.3688424577517784
9	0.2355429572068533	0.3395564308870595
>10	2.7383598785986334	10.627973096769326
>50	1.3158032742192765	15.463242698214447
>100	1.9152825631386359	52.26576570684872
>500	0.008806860259375809	0.920854626324237
>1k	0.0014678100432293014	0.42044199629160006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3873	0.11893334021199797	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05628835853565513	0.0
2	0.0	0.0	0.0	0.20083244125651092	0.0
3	0.0	0.0	0.0	0.2883818739816352	0.0
4	0.0	0.0	0.0	0.4436431619010418	0.0
5	0.0	0.0	0.0	0.7781796473824532	0.0
6	0.0	0.0	0.0	1.079336184294166	0.0
7	0.0	0.0	0.0	1.2426123448692226	0.0
8	0.0	0.0	0.0	1.615657069086974	0.0
9	0.0	0.0	0.0	1.7349589091911857	0.0
10	0.0	0.0	0.0	1.950347096190141	0.0
11	0.0	0.0	0.0	2.2727230852284976	0.0
12	0.0	0.0	0.0	2.534265883727229	0.0
13	0.0	0.0	0.0	2.6417757272805997	0.0
14	0.0	0.0	0.0	2.6897728382414448	0.0
15	0.0	0.0	0.0	2.7649775245774073	0.0
16	0.0	0.0	0.0	2.924630102879028	0.0
17	0.0	0.0	0.0	3.1068532995787432	0.0
18	0.0	0.0	0.0	3.318556487655561	0.0
19	3.070832435114846E-5	0.0	0.0	3.453673114800614	0.0
20	6.141664870229693E-5	0.0	0.0	3.593979448761011	0.0
21	6.141664870229693E-5	0.0	0.0	3.7739302294587413	0.0
22	6.141664870229693E-5	0.0	0.0	3.958180175565632	0.0
23	6.141664870229693E-5	0.0	0.0	4.150137911084661	0.0
24	9.212497305344538E-5	0.0	0.0	4.302881116407273	0.0
25	1.2283329740459385E-4	0.0	0.0	4.4413142425822505	0.0
26	1.8424994610689077E-4	0.0	0.0	4.575939536537685	0.0
27	1.8424994610689077E-4	0.0	0.0	4.709367205843425	0.0
28	1.8424994610689077E-4	0.0	0.0	4.843900374825807	0.0
29	1.8424994610689077E-4	0.0	0.0	4.9975955382033055	0.0
30	1.8424994610689077E-4	0.0	0.0	5.188754857289204	0.0
31	1.8424994610689077E-4	0.0	0.0	5.362748223062812	0.0
32	1.8424994610689077E-4	0.0	0.0	5.511591471192828	0.0
33	1.8424994610689077E-4	0.0	0.0	5.664365384839791	0.0
34	1.8424994610689077E-4	0.0	0.0	5.835871376340956	0.0
35	1.8424994610689077E-4	0.0	0.0	6.048403689175254	0.0
36	1.8424994610689077E-4	0.0	0.0	6.223533262949854	0.0
37	1.8424994610689077E-4	0.0	0.0	6.413617790683463	0.0
38	1.8424994610689077E-4	0.0	0.0	6.628054019627532	0.0
39	1.8424994610689077E-4	0.0	0.0	6.972232918955204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	7.035194E-4	45.0	29
CGTCGTT	25	3.892387E-5	45.0	29
ATACGAC	25	3.892387E-5	45.0	12
ACGCGTC	40	6.8212103E-9	45.0	35
TAGTACG	85	0.0	42.352943	1
TATTACG	70	0.0	41.785713	1
TACGATC	65	0.0	41.538464	11
TCGAACG	60	3.6379788E-12	41.250004	1
CGTATTT	620	0.0	41.008064	1
CGCATAT	55	6.184564E-11	40.909092	29
TACGGGA	850	0.0	40.764706	4
ACGTTAG	155	0.0	40.64516	1
TGTTACG	145	0.0	40.34483	1
ACGGGAT	1030	0.0	39.757282	5
CTATACG	80	0.0	39.375	1
TATAGCG	200	0.0	39.375	1
TACGTCA	40	3.4608274E-7	39.375	38
TAGACGC	35	6.251821E-6	38.571426	45
CCGTCGA	35	6.251821E-6	38.571426	19
CCGAATA	35	6.251821E-6	38.571426	38
>>END_MODULE