FastQCFastQC Report
Thu 26 May 2016
SRR1545288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545288_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2456797
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.10729417204596065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC302.1665146E-645.00000424
CGTAGTC302.1665146E-645.00000425
TAGTCGA351.2126839E-745.00000414
CGTACGT253.892107E-545.031
CACGTTA207.0348557E-445.019
ATACGCA207.0348557E-445.042
TATGTCG253.892107E-545.01
ATCCGTT501.0822987E-940.515
CGAAACG451.9297659E-840.00000413
TCGATCT403.4603727E-739.37540
TATCGGC403.4603727E-739.37524
TCACGAC1900.039.07894525
GTCCGAT356.2511917E-638.5714326
ATAGGGC12350.038.0769234
TAGCACG1300.038.076921
TAGTAGG10700.038.0607452
AGGGCGA21650.038.0369536
TAATGCG950.037.8947371
TCGATAG1250.037.8000031
ATACTCG301.1402997E-437.50000444
Produced by FastQC (version 0.11.5)