FastQCFastQC Report
Thu 26 May 2016
SRR1545287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545287_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1740313
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.1293445489403343No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21020.12078287066751786No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18010.10348713133786853No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8916347E-545.00000438
CTATCGA253.8916347E-545.00000425
CGCATTA502.1827873E-1145.00000423
GACCGTT253.8916347E-545.00000434
TAGCGAT351.212411E-745.00000424
TATGCGT302.1661472E-645.00000420
TCTAGCG351.212411E-745.0000041
GCCGATC351.212411E-745.0000049
TTAACGA253.8916347E-545.00000423
TATAGCG600.045.0000041
CGTCGCA302.1661472E-645.00000432
CCGAATA253.8916347E-545.00000422
AACGTAT207.034289E-445.040
ACCGGGT406.8193913E-945.04
CATGCGA207.034289E-445.028
TCCGATT207.034289E-445.026
CATTCGA207.034289E-445.028
CCCAACG207.034289E-445.023
CTACGTC207.034289E-445.030
AGTTATC207.034289E-445.021