##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545287_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1740313
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334484084184858	31.0	31.0	33.0	30.0	34.0
2	31.642798737928178	31.0	31.0	34.0	30.0	34.0
3	31.736932379405314	31.0	31.0	34.0	30.0	34.0
4	30.328922440963208	35.0	33.0	37.0	0.0	37.0
5	32.903463342513675	35.0	33.0	37.0	19.0	37.0
6	34.55312176602715	35.0	33.0	37.0	30.0	37.0
7	35.52567670298389	36.0	35.0	37.0	33.0	37.0
8	35.70515361317188	37.0	35.0	37.0	35.0	37.0
9	37.73375306625877	39.0	37.0	39.0	35.0	39.0
10	37.20840848743875	39.0	37.0	39.0	34.0	39.0
11	36.75739076821238	39.0	35.0	39.0	32.0	39.0
12	35.747023092972356	37.0	35.0	39.0	31.0	39.0
13	35.33527474655421	37.0	35.0	39.0	30.0	39.0
14	36.21159182285026	38.0	35.0	40.0	31.0	41.0
15	36.48243045934841	38.0	35.0	40.0	31.0	41.0
16	36.663091064653315	38.0	35.0	40.0	32.0	41.0
17	36.60077813588705	38.0	35.0	40.0	32.0	41.0
18	36.57072951819586	38.0	35.0	40.0	31.0	41.0
19	36.555230582084945	38.0	35.0	40.0	31.0	41.0
20	36.45892319370136	38.0	35.0	40.0	31.0	41.0
21	36.29061151643411	37.0	35.0	40.0	31.0	41.0
22	36.17815243579747	37.0	35.0	40.0	31.0	41.0
23	36.138891107519164	37.0	34.0	40.0	31.0	41.0
24	36.066927041285105	37.0	34.0	40.0	31.0	41.0
25	35.962171747266154	37.0	34.0	40.0	30.0	41.0
26	35.79043884634546	37.0	34.0	40.0	30.0	41.0
27	35.800099752171015	37.0	34.0	40.0	30.0	41.0
28	35.793061363099625	36.0	34.0	40.0	30.0	41.0
29	35.84501178810938	36.0	34.0	40.0	30.0	41.0
30	35.79246664249477	36.0	34.0	40.0	30.0	41.0
31	35.698545606451255	36.0	34.0	40.0	30.0	41.0
32	35.59067880318081	36.0	34.0	40.0	30.0	41.0
33	35.47412505681449	36.0	34.0	40.0	29.0	41.0
34	35.378602010098184	36.0	34.0	40.0	29.0	41.0
35	35.25851211822241	36.0	34.0	40.0	29.0	41.0
36	35.09218514140847	36.0	34.0	40.0	28.0	41.0
37	35.03186840528112	36.0	34.0	40.0	27.0	41.0
38	35.03688302046816	35.0	34.0	40.0	28.0	41.0
39	34.98279562354588	36.0	34.0	40.0	28.0	41.0
40	34.84105445399764	36.0	34.0	40.0	27.0	41.0
41	34.804550101045045	35.0	34.0	40.0	27.0	41.0
42	34.75359145165266	35.0	33.0	40.0	27.0	41.0
43	34.691094647916785	35.0	33.0	40.0	27.0	41.0
44	34.61911851488784	35.0	33.0	40.0	27.0	41.0
45	34.49133690318926	35.0	33.0	40.0	27.0	41.0
46	34.424097849065085	35.0	33.0	39.0	27.0	41.0
47	34.36386960276686	35.0	33.0	39.0	27.0	41.0
48	34.253310755019356	35.0	33.0	39.0	26.0	41.0
49	34.21052132576151	35.0	33.0	39.0	26.0	41.0
50	34.08484967933929	35.0	33.0	39.0	26.0	41.0
51	33.98762866220042	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	4.0
14	13.0
15	52.0
16	98.0
17	284.0
18	692.0
19	1276.0
20	2329.0
21	3745.0
22	5966.0
23	8710.0
24	12274.0
25	17136.0
26	22441.0
27	28124.0
28	34265.0
29	42386.0
30	52910.0
31	66431.0
32	85661.0
33	125369.0
34	189272.0
35	255172.0
36	133393.0
37	168405.0
38	218526.0
39	265192.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.28148442262972	25.02590051329847	29.36333866379209	12.329276400279719
2	28.84774175679892	26.621992710506674	30.82330592255531	13.706959610139096
3	29.013286690382706	26.192817039233745	30.615584667815504	14.178311602568044
4	22.966788158222112	37.83422867035987	26.1660977077112	13.032885463706817
5	23.33861782334557	32.886785308160086	29.443611580215745	14.330985288278603
6	22.780442368700342	41.313085634595616	25.36974670648326	10.536725290220781
7	90.02679403072895	4.3057771791625985	3.9305573192868177	1.7368714708216282
8	91.99368159635651	2.702789670593738	3.544132578449968	1.7593961545997758
9	88.26509943900896	4.652151653179629	4.653932941947799	2.428815965863612
10	58.978471114104195	22.32201908507263	9.181336920427533	9.518172880395653
11	50.07265934346293	20.185162094404856	15.765842121503432	13.976336440628783
12	45.872093123478365	20.556244767464243	19.326293603506954	14.245368505550438
13	23.247312408744865	39.143935602388765	19.789083917663085	17.819668071203285
14	16.53369250244065	40.722099990059256	24.143415580990315	18.60079192650977
15	16.343726674454537	22.56157369392747	44.84400219960432	16.250697432013666
16	18.672100938164572	17.680382781718002	43.17085489794078	20.476661382176655
17	18.567579510122602	20.96289575495902	24.913564399047758	35.555960335870616
18	23.491751196480173	25.33159265028762	29.127978702681645	22.04867745055056
19	33.03658594747037	25.808575813661104	20.609511047725324	20.545327191143205
20	37.09895863560176	22.962076362125664	21.216585752103214	18.722379250169364
21	26.709907930355058	27.994906663341595	23.31666774884748	21.97851765745587
22	25.65624689351858	25.421059315192153	21.6421988458398	27.28049494544947
23	24.754167784760558	29.839632296029507	21.25726808913109	24.148931830078844
24	23.622877034188676	22.524453934435932	34.494599534681406	19.358069496693986
25	21.048512537687188	24.525818056866783	30.750962614196414	23.67470679124962
26	19.977613222449065	35.723171636366565	23.32465481784024	20.974560323344136
27	21.99069937419303	33.64699338567258	25.307114294957287	19.0551929451771
28	18.19167011911076	29.041959693457443	34.79615448485416	17.97021570257764
29	19.849475352996844	25.275223479914246	32.82415289663411	22.0511482704548
30	22.926852813258307	30.13337255999352	26.31808186228569	20.621692764462484
31	31.885930864160645	26.771161279608897	20.863545810437547	20.47936204579291
32	34.035429258989616	27.342322903983362	21.395921308408315	17.226326528618703
33	31.36332372395081	28.107874847800367	21.021735745236633	19.507065683012193
34	22.407693328728797	27.52591056896087	25.327340541615218	24.739055560695117
35	24.793068833020268	24.32878453473599	27.44253476242492	23.43561186981882
36	31.27759201936663	26.903896023301556	22.25398534631414	19.564526611017673
37	23.80318942626987	33.56516902419278	24.547308443940832	18.084333105596524
38	23.582482001800827	32.469676431768306	20.968182160335523	22.97965940609534
39	24.70521107409989	31.649134379850064	22.85387743469135	20.7917771113587
40	27.135061336667597	24.81524875123038	23.71056241032504	24.33912750177698
41	18.257807647245063	25.205925600739636	25.904535563430258	30.631731188585043
42	23.15405332259197	26.215743949507935	21.760453435675075	28.869749292225023
43	23.636495274125977	25.93073774660076	23.99269556683194	26.440071412441323
44	22.48290968348797	29.64501213287495	25.313837223533923	22.558240960103156
45	20.590893707051546	34.60768264099619	20.531191802853854	24.27023184909841
46	22.58513267440972	32.44899049768634	23.128310826845517	21.83756600105843
47	23.981777990510903	26.329056899534738	24.987861378958844	24.701303730995516
48	25.221095285733085	20.997946921042367	29.881751156257526	23.899206636967026
49	21.46562141407896	24.428191940185474	29.897897677027064	24.2082889687085
50	20.204698809926718	31.8813339899202	25.066238084758318	22.84772911539476
51	19.96738517726409	32.527769429981845	22.704823787445132	24.80002160530893
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	642.0
2	1057.0
3	2949.5
4	4842.0
5	3615.5
6	2389.0
7	2574.0
8	2759.0
9	2881.0
10	3003.0
11	2958.0
12	2913.0
13	3014.0
14	3115.0
15	2795.5
16	2476.0
17	2520.5
18	2565.0
19	2617.0
20	2669.0
21	2951.5
22	3234.0
23	3524.0
24	3814.0
25	4479.5
26	8505.5
27	11866.0
28	14860.5
29	17855.0
30	19871.0
31	21887.0
32	26551.0
33	31215.0
34	34543.0
35	37871.0
36	38674.5
37	39478.0
38	49284.5
39	59091.0
40	89199.0
41	119307.0
42	137816.0
43	156325.0
44	158864.0
45	161403.0
46	150906.5
47	140410.0
48	129594.0
49	118778.0
50	114234.5
51	109691.0
52	102943.0
53	96195.0
54	90909.5
55	85624.0
56	87554.0
57	89484.0
58	95560.5
59	101637.0
60	98087.0
61	94537.0
62	81274.5
63	68012.0
64	57761.5
65	47511.0
66	40021.5
67	32532.0
68	27465.0
69	22398.0
70	18346.0
71	14294.0
72	12406.0
73	10518.0
74	8084.0
75	4423.5
76	3197.0
77	2358.0
78	1519.0
79	1281.5
80	1044.0
81	716.5
82	389.0
83	295.0
84	201.0
85	156.0
86	111.0
87	87.0
88	63.0
89	37.0
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1740313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.76458681648982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5540180787183	13.169256558647838
2	8.162945743024292	2.7369682517445346
3	2.56899926378243	1.2920463356753693
4	1.1837006445755254	0.7937700888288542
5	0.6802296668122637	0.570188465221307
6	0.508780836291477	0.5117700300344856
7	0.3157498928876582	0.3705391544128755
8	0.2814141183416927	0.3774233134660198
9	0.22899946133844978	0.34551732154840603
>10	3.9157390261238434	17.009838118648595
>50	2.055097681382306	25.20683732006259
>100	1.5375703350665404	36.445919926257204
>500	0.0045035011034411715	0.5259787020800205
>1k	0.0022517505517205857	0.6439464133718913
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2251	0.1293445489403343	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2102	0.12078287066751786	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1801	0.10348713133786853	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05677139686941372	0.0
2	0.0	0.0	0.0	0.2088130123719124	0.0
3	0.0	0.0	0.0	0.29351042025198915	0.0
4	0.0	0.0	0.0	0.4353239905695125	0.0
5	0.0	0.0	0.0	0.7441190176709592	0.0
6	0.0	0.0	0.0	1.0158517462088716	0.0
7	0.0	0.0	0.0	1.1673187524313156	0.0
8	0.0	0.0	0.0	1.5123716251042199	0.0
9	0.0	0.0	0.0	1.619536255834439	0.0
10	0.0	0.0	0.0	1.8041007565880391	0.0
11	0.0	0.0	0.0	2.108241448521042	0.0
12	0.0	0.0	0.0	2.334752426718642	0.0
13	0.0	0.0	0.0	2.4275518254474915	0.0
14	0.0	0.0	0.0	2.4653036551470913	0.0
15	0.0	0.0	0.0	2.525580168624839	0.0
16	0.0	0.0	0.0	2.6816440490877214	0.0
17	0.0	0.0	0.0	2.854831286096237	0.0
18	0.0	0.0	0.0	3.0392808649938257	0.0
19	0.0	0.0	0.0	3.146905183148089	0.0
20	5.746092800547948E-5	0.0	0.0	3.2617695782310423	0.0
21	5.746092800547948E-5	0.0	0.0	3.4274868945988453	0.0
22	5.746092800547948E-5	0.0	0.0	3.601133819031404	0.0
23	1.1492185601095895E-4	0.0	0.0	3.7921914046496235	0.0
24	1.1492185601095895E-4	0.0	0.0	3.93676309951141	0.0
25	1.1492185601095895E-4	0.0	0.0	4.059786946371141	0.0
26	1.1492185601095895E-4	0.0	0.0	4.168043334733465	0.0
27	1.1492185601095895E-4	0.0	0.0	4.274403512471607	0.0
28	1.1492185601095895E-4	0.0	0.0	4.388233610850462	0.0
29	1.1492185601095895E-4	0.0	0.0	4.515509566382599	0.0
30	1.1492185601095895E-4	0.0	0.0	4.662954307644659	0.0
31	1.1492185601095895E-4	0.0	0.0	4.82183377357981	0.0
32	1.1492185601095895E-4	0.0	0.0	4.97105980361004	0.0
33	1.1492185601095895E-4	0.0	0.0	5.10580567978289	0.0
34	1.1492185601095895E-4	0.0	0.0	5.252848194548911	0.0
35	1.1492185601095895E-4	0.0	0.0	5.434424727046227	0.0
36	1.1492185601095895E-4	0.0	0.0	5.612783447575235	0.0
37	1.1492185601095895E-4	0.0	0.0	5.791372011816265	0.0
38	1.1492185601095895E-4	0.0	0.0	5.952837219511663	0.0
39	1.1492185601095895E-4	0.0	0.0	6.121312660423729	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8916347E-5	45.000004	38
CTATCGA	25	3.8916347E-5	45.000004	25
CGCATTA	50	2.1827873E-11	45.000004	23
GACCGTT	25	3.8916347E-5	45.000004	34
TAGCGAT	35	1.212411E-7	45.000004	24
TATGCGT	30	2.1661472E-6	45.000004	20
TCTAGCG	35	1.212411E-7	45.000004	1
GCCGATC	35	1.212411E-7	45.000004	9
TTAACGA	25	3.8916347E-5	45.000004	23
TATAGCG	60	0.0	45.000004	1
CGTCGCA	30	2.1661472E-6	45.000004	32
CCGAATA	25	3.8916347E-5	45.000004	22
AACGTAT	20	7.034289E-4	45.0	40
ACCGGGT	40	6.8193913E-9	45.0	4
CATGCGA	20	7.034289E-4	45.0	28
TCCGATT	20	7.034289E-4	45.0	26
CATTCGA	20	7.034289E-4	45.0	28
CCCAACG	20	7.034289E-4	45.0	23
CTACGTC	20	7.034289E-4	45.0	30
AGTTATC	20	7.034289E-4	45.0	21
>>END_MODULE