FastQCFastQC Report
Thu 26 May 2016
SRR1545286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545286_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3295481
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG56900.17266068291700057No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT38730.1175245738027317No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.11233564994002393No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG36450.11060600865245467No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA207.0352055E-445.00000426
TACGTCG207.0352055E-445.00000444
TACGATA351.2128476E-745.031
TATTACG351.2128476E-745.01
CTCGCTA253.8923972E-545.021
ACGTAAG950.045.01
CGAACTA302.166742E-644.99999614
ACGTCAC302.166742E-644.99999616
AATCGGC302.166742E-644.99999635
TATAGCG1450.041.8965531
CGTATTT6500.040.51
TCGATAG2000.040.51
GTACGTA501.0822987E-940.523
ATAGGGT7250.040.034484
CGTTTCA451.9301297E-840.025
TAGTACG451.9301297E-840.01
CGTTAGG3950.039.8734172
CGTCGAT403.4608456E-739.37500420
ATCTACC11700.039.2307715
ATACCGG1050.038.571432
Produced by FastQC (version 0.11.5)