FastQCFastQC Report
Thu 26 May 2016
SRR1545285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545285_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3160799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG59660.1887497433402124No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.1101303815902245No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC32080.10149332494726808No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA207.035162E-445.00000426
ATCTCGC207.035162E-445.00000413
CGTATCA207.035162E-445.00000430
CCGTATC207.035162E-445.00000429
CGTACGT207.035162E-445.00000413
TCCGTCG406.8212103E-945.00000412
CGACTTA600.045.00000438
TAGTCCG351.2128294E-745.00000440
TACCGAA207.035162E-445.00000425
CTATACG453.8562575E-1045.01
TTATCCG253.892361E-545.01
CGCTAAG502.1827873E-1145.022
GTCGTAC253.892361E-545.020
CGACTAT253.892361E-545.034
CGATATC253.892361E-545.010
TCGCGAC253.892361E-545.038
ACGACAC253.892361E-545.015
ACGGTAC253.892361E-545.013
GCGATAT2500.043.29
TCATGCA9600.042.4218824