##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545285_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3160799
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234123397280246	31.0	31.0	33.0	30.0	34.0
2	31.54898935364128	31.0	31.0	34.0	30.0	34.0
3	31.647211037462363	31.0	31.0	34.0	30.0	34.0
4	30.276679092849623	35.0	33.0	37.0	0.0	37.0
5	32.827966915960175	35.0	33.0	37.0	19.0	37.0
6	34.4399197797772	35.0	33.0	37.0	30.0	37.0
7	35.43963440889471	36.0	35.0	37.0	33.0	37.0
8	35.6353497960484	37.0	35.0	37.0	35.0	37.0
9	37.64679690166948	39.0	37.0	39.0	35.0	39.0
10	37.09837987167169	39.0	37.0	39.0	33.0	39.0
11	36.66604614845803	39.0	35.0	39.0	32.0	39.0
12	35.600603834663325	37.0	35.0	39.0	31.0	39.0
13	35.15054895929795	37.0	35.0	39.0	30.0	39.0
14	35.984706714979346	38.0	35.0	40.0	30.0	41.0
15	36.309316410186156	38.0	35.0	40.0	31.0	41.0
16	36.48644820502664	38.0	35.0	40.0	32.0	41.0
17	36.42342268521345	38.0	35.0	40.0	31.0	41.0
18	36.38263806081943	37.0	35.0	40.0	31.0	41.0
19	36.39236471537735	37.0	35.0	40.0	31.0	41.0
20	36.292657647639096	37.0	35.0	40.0	31.0	41.0
21	36.12607476780396	37.0	34.0	40.0	31.0	41.0
22	35.98839533927972	37.0	34.0	40.0	30.0	41.0
23	35.95736679238382	37.0	34.0	40.0	30.0	41.0
24	35.88011069353034	36.0	34.0	40.0	30.0	41.0
25	35.75939248272351	36.0	34.0	40.0	30.0	41.0
26	35.58494102282366	36.0	34.0	40.0	30.0	41.0
27	35.57519190559096	36.0	34.0	40.0	30.0	41.0
28	35.60256536401081	36.0	34.0	40.0	30.0	41.0
29	35.631764626602326	36.0	34.0	40.0	30.0	41.0
30	35.59581042641433	36.0	34.0	40.0	30.0	41.0
31	35.494289576781064	36.0	34.0	40.0	30.0	41.0
32	35.397121740420694	36.0	34.0	40.0	29.0	41.0
33	35.25145445819238	36.0	34.0	40.0	29.0	41.0
34	35.15730737702714	36.0	34.0	40.0	29.0	41.0
35	35.04249748244036	36.0	34.0	40.0	28.0	41.0
36	34.89750724421262	36.0	33.0	40.0	27.0	41.0
37	34.837184522014844	35.0	33.0	40.0	27.0	41.0
38	34.827157310540784	35.0	33.0	40.0	27.0	41.0
39	34.77182636415666	35.0	33.0	40.0	27.0	41.0
40	34.62213573213608	35.0	33.0	40.0	26.0	41.0
41	34.57934623492351	35.0	33.0	40.0	26.0	41.0
42	34.55629162120084	35.0	33.0	40.0	27.0	41.0
43	34.4833201984688	35.0	33.0	40.0	27.0	41.0
44	34.41885928209924	35.0	33.0	40.0	27.0	41.0
45	34.32244252165354	35.0	33.0	39.0	26.0	41.0
46	34.251132387728546	35.0	33.0	39.0	26.0	41.0
47	34.17884528563822	35.0	33.0	39.0	26.0	41.0
48	34.09307488391384	35.0	33.0	39.0	26.0	41.0
49	34.064770015429644	35.0	33.0	39.0	26.0	41.0
50	33.893248510898665	35.0	33.0	39.0	26.0	40.0
51	33.824882569249105	35.0	33.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	12.0
14	22.0
15	85.0
16	276.0
17	639.0
18	1461.0
19	2820.0
20	5070.0
21	8326.0
22	12617.0
23	18240.0
24	25920.0
25	35119.0
26	45216.0
27	55649.0
28	66934.0
29	81411.0
30	100590.0
31	125468.0
32	160528.0
33	232130.0
34	346049.0
35	463649.0
36	233811.0
37	293414.0
38	377943.0
39	467123.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.543796995633066	25.137441513996933	29.208437486850634	12.110324003519363
2	28.447996851429018	27.143105271799943	30.60529948282064	13.80359839395039
3	29.877635370044093	25.744123558631856	30.143707334759345	14.23453373656471
4	22.807998863578483	38.61188262841136	25.779810737728027	12.800307770282135
5	24.104854500396893	32.52886374616038	28.988967662923205	14.377314090519516
6	22.484441433953883	41.45470812917873	24.947331355141532	11.113519081725855
7	88.97082668021599	4.684385182354209	4.327513391392493	2.0172747460373155
8	90.76458199335042	3.2594922992572446	3.901988073268816	2.073937634123524
9	86.89805963618693	4.866870686810518	5.310049769061557	2.925019907940998
10	57.97100669798997	22.205334790348896	9.674136191513602	10.149522320147533
11	51.45309777685958	20.478176562318577	15.905820015761837	12.162905645059999
12	47.73859394412615	20.35912438595431	18.35871246479134	13.543569205128197
13	23.613681224272725	40.31872953642417	18.918855643778677	17.148733595524423
14	14.834097327922466	42.81873032736343	24.232543733404118	18.11462861130999
15	14.936919430814804	23.658226923002694	45.82145210752092	15.583401538661585
16	16.64110878293748	18.25813662937757	45.76225821382505	19.3384963738599
17	17.80119520412402	19.536136274404033	26.48311392151162	36.179554599960326
18	21.749152666778244	25.668731229034176	29.705020787465447	22.877095316722134
19	33.0732514152276	25.661043299494846	20.759118184990566	20.506587100286986
20	36.7131854951865	23.43670065701742	20.791135405952733	19.058978441843344
21	26.109189480254834	28.576382110978898	23.225962802443306	22.088465606322956
22	23.746052817657816	27.056829618080748	22.075494202573463	27.12162336168798
23	24.192395656920922	31.214797271196304	21.47732266430102	23.115484407581754
24	23.630006210455015	22.977101675873726	34.01054606762404	19.382346046047218
25	20.426797148442528	24.888865125558443	32.08748800540623	22.5968497205928
26	19.271456362774096	36.92781477088546	22.692205356936647	21.108523509403792
27	21.47156462653905	34.57600435839166	25.379342375139956	18.573088639929335
28	18.296987565485814	30.227135607167682	34.3473280015591	17.128548825787405
29	18.384528722009847	26.185341111535404	33.515291544954295	21.914838621500447
30	21.877601201468362	30.13522846596699	26.63019698500284	21.3569733475618
31	30.986975128757	27.189043023615234	20.518577739362737	21.30540410826503
32	34.53484387966461	27.30154622296451	20.907276925865897	17.25633297150499
33	30.93018569039031	28.077679093166	21.092894549764157	19.899240666679532
34	21.482005024678887	28.47960278397962	25.0233247985715	25.01506739276999
35	22.50396181471837	25.110454666683964	27.870294821024682	24.515288697572988
36	30.408671984520367	27.605203620983175	21.722482195166474	20.26364219932998
37	23.549615144778265	33.5965368250243	24.53003180524924	18.323816224948185
38	22.332739285225035	33.33388171788209	21.298412205268352	23.034966791624523
39	23.32369125654621	31.838310503135443	23.63063896185743	21.20735927846092
40	26.499185807132942	24.744724356088444	24.054455851194586	24.701633985584024
41	17.712894745917094	24.993585482658023	26.38522727955811	30.908292491866774
42	23.703785023976533	27.25140067432317	21.12162146343377	27.923192838266527
43	23.673697694791727	27.717295531920882	22.79961490749649	25.8093918657909
44	21.774842373716265	30.510703148159692	25.17303378038275	22.541420697741298
45	19.677714400694253	34.88921630258678	19.93609843587017	25.496970860848794
46	22.04021831188886	34.05891991233862	21.916072486735157	21.98478928903736
47	23.27576033781332	26.516459920418857	25.117984408372696	25.08979533339513
48	24.370958102682263	21.706979785807324	29.843972995435646	24.078089116074764
49	21.014844664276342	24.790503920053126	29.760829461158394	24.433821954512133
50	18.804359277511793	33.180249677375876	24.308885190105414	23.706505855006917
51	18.373613760318197	33.66553836545759	22.367414062077344	25.593433812146866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	575.0
1	1200.5
2	1826.0
3	4767.5
4	7709.0
5	5766.5
6	3824.0
7	4141.5
8	4459.0
9	4905.0
10	5351.0
11	5413.5
12	5476.0
13	5411.0
14	5346.0
15	5504.5
16	5663.0
17	5945.5
18	6228.0
19	6478.0
20	6728.0
21	7499.0
22	8270.0
23	9387.0
24	10504.0
25	10937.0
26	16131.0
27	20892.0
28	31618.5
29	42345.0
30	43525.0
31	44705.0
32	48365.0
33	52025.0
34	56560.5
35	61096.0
36	65909.0
37	70722.0
38	89308.0
39	107894.0
40	163233.0
41	218572.0
42	258131.5
43	297691.0
44	300464.0
45	303237.0
46	285094.0
47	266951.0
48	250927.0
49	234903.0
50	220939.0
51	206975.0
52	191623.0
53	176271.0
54	168968.0
55	161665.0
56	162372.0
57	163079.0
58	164152.5
59	165226.0
60	160131.5
61	155037.0
62	136843.5
63	118650.0
64	98527.0
65	78404.0
66	62162.5
67	45921.0
68	38554.5
69	31188.0
70	27598.5
71	24009.0
72	19208.0
73	14407.0
74	10855.5
75	5854.5
76	4405.0
77	3287.0
78	2169.0
79	1695.5
80	1222.0
81	766.5
82	311.0
83	208.0
84	105.0
85	76.5
86	48.0
87	34.5
88	21.0
89	16.0
90	11.0
91	9.5
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3160799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.923946183642087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58713923550434	11.579062904934121
2	9.562705824564281	2.854266141915961
3	3.4459023801222766	1.5427938502508711
4	1.7142946301842523	1.0233616321510557
5	1.0074214511226505	0.7517351760400527
6	0.6437551795203957	0.5764420592761943
7	0.4346432058696722	0.454061426943926
8	0.31413432647247813	0.3750499026167934
9	0.23777844689587888	0.3193733470593685
>10	2.0108478046906706	7.023362179508363
>50	0.9752459508323131	10.742999900286717
>100	2.0372038710510783	59.135019899805194
>500	0.024762105353206432	2.3337149579437657
>1k	0.00393416627107018	1.0827075256630674
>5k	2.3142154535706942E-4	0.2060490956045124
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5966	0.1887497433402124	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3481	0.1101303815902245	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3208	0.10149332494726808	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06615415912242442	0.0
2	0.0	0.0	0.0	0.21874215981465445	0.0
3	6.327514024143895E-5	0.0	0.0	0.31144024026836253	0.0
4	6.327514024143895E-5	0.0	0.0	0.5352444113023321	0.0
5	6.327514024143895E-5	0.0	0.0	0.9017972987209879	0.0
6	6.327514024143895E-5	0.0	0.0	1.2556002453809938	0.0
7	6.327514024143895E-5	0.0	0.0	1.4324542623558156	0.0
8	6.327514024143895E-5	0.0	0.0	1.8683883410492095	0.0
9	6.327514024143895E-5	0.0	0.0	1.989212221340237	0.0
10	6.327514024143895E-5	0.0	0.0	2.2156106731241056	0.0
11	9.491271036215843E-5	0.0	0.0	2.5402121425626873	0.0
12	9.491271036215843E-5	0.0	0.0	2.814098587097756	0.0
13	9.491271036215843E-5	0.0	0.0	2.923184928873997	0.0
14	9.491271036215843E-5	0.0	0.0	2.9672877016222796	0.0
15	9.491271036215843E-5	0.0	0.0	3.0418574543968155	0.0
16	9.491271036215843E-5	0.0	0.0	3.202165022198501	0.0
17	9.491271036215843E-5	0.0	0.0	3.3728180754296617	0.0
18	9.491271036215843E-5	0.0	0.0	3.5772916911198718	0.0
19	9.491271036215843E-5	0.0	0.0	3.7086192446909783	0.0
20	9.491271036215843E-5	0.0	0.0	3.8376688932133933	0.0
21	9.491271036215843E-5	0.0	0.0	4.018857257294754	0.0
22	9.491271036215843E-5	0.0	0.0	4.201247849040701	0.0
23	9.491271036215843E-5	0.0	0.0	4.3973058710788	0.0
24	9.491271036215843E-5	0.0	0.0	4.540940439426866	0.0
25	9.491271036215843E-5	0.0	0.0	4.663188010373326	0.0
26	9.491271036215843E-5	0.0	0.0	4.78075322094192	0.0
27	9.491271036215843E-5	0.0	0.0	4.889618099727316	0.0
28	9.491271036215843E-5	0.0	0.0	5.005949445061201	0.0
29	9.491271036215843E-5	0.0	0.0	5.131076034888647	0.0
30	9.491271036215843E-5	0.0	0.0	5.285056088666188	0.0
31	9.491271036215843E-5	0.0	0.0	5.4531464987175715	0.0
32	9.491271036215843E-5	0.0	0.0	5.6022860042666425	0.0
33	9.491271036215843E-5	0.0	0.0	5.74158622550817	0.0
34	9.491271036215843E-5	0.0	0.0	5.891263569749294	0.0
35	9.491271036215843E-5	0.0	0.0	6.077640495330453	0.0
36	9.491271036215843E-5	0.0	0.0	6.258860496981934	0.0
37	1.265502804828779E-4	0.0	0.0	6.441662377139451	0.0
38	1.5818785060359739E-4	0.0	0.0	6.5956424309169925	0.0
39	1.5818785060359739E-4	0.0	0.0	6.764650330501876	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	20	7.035162E-4	45.000004	26
ATCTCGC	20	7.035162E-4	45.000004	13
CGTATCA	20	7.035162E-4	45.000004	30
CCGTATC	20	7.035162E-4	45.000004	29
CGTACGT	20	7.035162E-4	45.000004	13
TCCGTCG	40	6.8212103E-9	45.000004	12
CGACTTA	60	0.0	45.000004	38
TAGTCCG	35	1.2128294E-7	45.000004	40
TACCGAA	20	7.035162E-4	45.000004	25
CTATACG	45	3.8562575E-10	45.0	1
TTATCCG	25	3.892361E-5	45.0	1
CGCTAAG	50	2.1827873E-11	45.0	22
GTCGTAC	25	3.892361E-5	45.0	20
CGACTAT	25	3.892361E-5	45.0	34
CGATATC	25	3.892361E-5	45.0	10
TCGCGAC	25	3.892361E-5	45.0	38
ACGACAC	25	3.892361E-5	45.0	15
ACGGTAC	25	3.892361E-5	45.0	13
GCGATAT	250	0.0	43.2	9
TCATGCA	960	0.0	42.42188	24
>>END_MODULE