FastQCFastQC Report
Thu 26 May 2016
SRR1545284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545284_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2278566
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34150.14987496521935287No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24710.10844539943104567No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.10730433088179145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA253.8920163E-545.016
GTCGATC351.2126293E-745.010
CGACGAA253.8920163E-545.010
CGCGAAA207.0347486E-445.026
CGTTCGC207.0347486E-445.041
TAGTTCG253.8920163E-545.01
CGTTACA253.8920163E-545.031
GGGCGTA207.0347486E-445.07
CGCTAAC207.0347486E-445.025
GAACCGT253.8920163E-545.018
CAATCGA253.8920163E-545.022
TCGACAC207.0347486E-445.034
TATCGTC253.8920163E-545.033
TCGAATC453.8562575E-1045.017
TACCGTA207.0347486E-445.045
TCTAGCG600.044.9999961
TACGTAG1400.043.3928571
CGGGATA1900.041.447376
TGCGTAG1150.041.086961
CGTTGAT3700.040.74324425
Produced by FastQC (version 0.11.5)