##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545284_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2278566
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22565596081044	31.0	31.0	33.0	30.0	34.0
2	31.544226939224057	31.0	31.0	34.0	30.0	34.0
3	31.646229690077003	31.0	31.0	34.0	30.0	34.0
4	30.301777521476225	35.0	33.0	37.0	0.0	37.0
5	32.83548293093112	35.0	33.0	37.0	19.0	37.0
6	34.453291675553835	35.0	33.0	37.0	30.0	37.0
7	35.4588486793887	36.0	35.0	37.0	33.0	37.0
8	35.64221356765615	37.0	35.0	37.0	35.0	37.0
9	37.647683674732264	39.0	37.0	39.0	35.0	39.0
10	37.0931704414092	39.0	37.0	39.0	33.0	39.0
11	36.65055170664356	39.0	35.0	39.0	32.0	39.0
12	35.5169303851633	37.0	35.0	39.0	31.0	39.0
13	35.05785744191741	37.0	34.0	39.0	30.0	39.0
14	35.86401140015255	37.0	34.0	40.0	30.0	41.0
15	36.1813482690429	37.0	35.0	40.0	31.0	41.0
16	36.37961419594605	37.0	35.0	40.0	31.0	41.0
17	36.307551767207976	37.0	35.0	40.0	31.0	41.0
18	36.28187070288945	37.0	35.0	40.0	31.0	41.0
19	36.27634924772862	37.0	35.0	40.0	31.0	41.0
20	36.157919498491594	37.0	35.0	40.0	31.0	41.0
21	36.00113887418666	37.0	34.0	40.0	30.0	41.0
22	35.84758132966085	36.0	34.0	40.0	30.0	41.0
23	35.79680421809155	36.0	34.0	40.0	30.0	41.0
24	35.73046644249058	36.0	34.0	40.0	30.0	41.0
25	35.59543546247947	36.0	34.0	40.0	30.0	41.0
26	35.42860334087316	36.0	34.0	40.0	29.0	41.0
27	35.412573083246215	36.0	34.0	40.0	29.0	41.0
28	35.43339407328995	36.0	34.0	40.0	29.0	41.0
29	35.498461312948585	36.0	34.0	40.0	30.0	41.0
30	35.445783883372265	36.0	34.0	40.0	29.0	41.0
31	35.34933462537403	36.0	34.0	40.0	29.0	41.0
32	35.23013158275863	35.0	34.0	40.0	29.0	41.0
33	35.08862898858317	35.0	34.0	40.0	29.0	41.0
34	34.98289231033905	35.0	34.0	40.0	28.0	41.0
35	34.87064320278631	35.0	34.0	40.0	27.0	41.0
36	34.69412384806935	35.0	33.0	40.0	26.0	41.0
37	34.64593652323435	35.0	33.0	40.0	27.0	41.0
38	34.61670893009024	35.0	33.0	40.0	27.0	41.0
39	34.569771953061704	35.0	33.0	40.0	27.0	41.0
40	34.430468987951194	35.0	33.0	40.0	26.0	41.0
41	34.430941214781576	35.0	33.0	40.0	26.0	41.0
42	34.383431070243304	35.0	33.0	40.0	26.0	41.0
43	34.311419111844906	35.0	33.0	39.0	26.0	41.0
44	34.22902035754066	35.0	33.0	39.0	26.0	41.0
45	34.12552807335842	35.0	33.0	39.0	26.0	41.0
46	34.058447725455395	35.0	33.0	39.0	26.0	41.0
47	34.0006253933395	35.0	33.0	39.0	26.0	41.0
48	33.910046933027175	35.0	33.0	39.0	25.0	41.0
49	33.8925332862862	35.0	33.0	39.0	26.0	41.0
50	33.720896827215014	35.0	33.0	38.0	24.0	40.0
51	33.636771109548725	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	5.0
14	24.0
15	66.0
16	186.0
17	445.0
18	1094.0
19	2246.0
20	3937.0
21	6352.0
22	9642.0
23	13729.0
24	19322.0
25	25993.0
26	34024.0
27	41734.0
28	50186.0
29	60363.0
30	74637.0
31	92684.0
32	118325.0
33	172139.0
34	258454.0
35	343548.0
36	167225.0
37	207425.0
38	263604.0
39	310988.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.391249584168285	24.715149791579442	30.56014177337852	12.333458850873752
2	27.66112546224248	27.419526140563843	31.406112440894844	13.51323595629883
3	29.065780846374427	26.415254155464446	30.542235774605608	13.976729223555518
4	22.639151115218958	38.78829930754694	25.65819906028616	12.91435051694794
5	23.96463389693342	32.4029235931722	29.28885974775363	14.34358276214075
6	22.5542731700552	41.481045534779334	25.10105039748684	10.863630897678627
7	89.50629474853922	4.534079767713553	4.0749313383944115	1.8846941453528228
8	91.68591122662237	2.9605901255438725	3.455594439660734	1.8979042081730353
9	88.14258617042474	4.473822570862551	4.939949073232901	2.4436421854798147
10	59.55254313458552	21.89425278881542	8.923507153183188	9.629696923415867
11	52.2895101568267	19.328911253832455	14.962173577592223	13.419405011748617
12	46.25970895730034	21.745518892145324	18.26148551325702	13.733286637297319
13	22.714110541454584	41.36786031214369	19.21756929577638	16.70045985062535
14	15.763905895198999	42.92462891134161	24.956178578983447	16.355286614475947
15	15.430143344542138	22.883471446515045	46.326022594912764	15.360362614030052
16	17.728079853732567	17.280824869676806	45.12496017231891	19.86613510427172
17	17.976788910218094	19.129926453743277	26.003196747427985	36.890087888610644
18	23.45304020160048	23.944445761062003	30.289445203693905	22.313068833643616
19	32.57386443930086	24.918567204109955	20.95787438239665	21.54969397419254
20	35.611520579171284	23.3573659924707	21.191222900719136	19.839890527638875
21	25.676895029593172	27.98027355801851	23.914295219010555	22.428536193377766
22	25.52083196185671	25.648456090365606	21.811481431742596	27.019230516035087
23	23.886119603294354	30.527533545220987	21.73059722650123	23.85574962498343
24	23.50008733563127	22.220422844894554	35.020315408901915	19.259174410572264
25	20.33581647404552	24.519368760878553	32.25717402963092	22.88764073544501
26	20.27200440979107	34.81496695728804	23.600325818958066	21.312702813962815
27	20.753272014064986	33.08194715448225	25.5702490074898	20.594531823962967
28	17.177514278717403	29.378389741618193	35.96156530028097	17.482530679383437
29	19.02108606904518	24.138866286954162	34.562132499124445	22.277915144876207
30	20.960639279265994	29.971394289215237	27.518404119081914	21.54956231243686
31	31.874564967615598	25.89391749021095	20.750463229943744	21.48105431222971
32	34.00230671395957	26.166501211727024	21.80024629525763	18.030945779055774
33	30.198861915783876	27.38191476569035	21.968466131768842	20.450757186756935
34	21.387223367679496	26.992020419860562	26.338144253885996	25.28261195857395
35	22.767345778002483	24.85291187527594	28.636431861091584	23.743310485629998
36	31.207347077065133	24.92111266471983	23.48608730227696	20.385452955938078
37	23.719743031362707	31.837216916253468	25.810575598863494	18.632464453520328
38	22.76493197914829	32.6158206521119	20.926494997292156	23.692752371447657
39	24.217819453112178	30.891051652662245	23.827398460259655	21.063730433965926
40	25.31912615215008	25.55308909199909	23.372463207122372	25.755321548728453
41	18.26438207188205	24.540171318276496	25.15160851166918	32.04383809817227
42	23.110456313312845	26.132137493493712	21.839876483718268	28.917529709475172
43	23.350519581175178	26.170626613405094	24.122408567493768	26.356445237925957
44	21.74608942641995	29.682923382513387	26.005259448267026	22.565727742799638
45	19.385920794043273	34.60457147170633	21.062852688928036	24.946655045322366
46	22.738599628011652	32.37088589928929	23.89551147519975	20.995002997499306
47	23.694551748775325	25.558926096501043	26.024525951848666	24.721996202874966
48	24.57475447276928	21.19275895453544	29.63091698901853	24.601569583676753
49	21.621230194780402	22.575251276460722	30.4279972579245	25.375521270834376
50	19.22946274103976	32.017593521539425	25.446179746384352	23.306763991036465
51	18.258896165395253	33.824914441802434	22.789772163720514	25.1264172290818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	272.0
1	763.0
2	1254.0
3	3417.5
4	5581.0
5	4284.0
6	2987.0
7	3189.0
8	3391.0
9	3616.0
10	3841.0
11	3893.5
12	3946.0
13	3978.0
14	4010.0
15	3708.5
16	3407.0
17	3544.5
18	3682.0
19	3344.0
20	3006.0
21	3679.0
22	4352.0
23	4547.0
24	4742.0
25	6862.5
26	10930.0
27	12877.0
28	16748.5
29	20620.0
30	23955.0
31	27290.0
32	31288.0
33	35286.0
34	38527.5
35	41769.0
36	48149.5
37	54530.0
38	67753.0
39	80976.0
40	121247.5
41	161519.0
42	188362.0
43	215205.0
44	218758.0
45	222311.0
46	204719.0
47	187127.0
48	179377.0
49	171627.0
50	162994.0
51	154361.0
52	147062.0
53	139763.0
54	129615.0
55	119467.0
56	116013.0
57	112559.0
58	116186.0
59	119813.0
60	114258.5
61	108704.0
62	92792.5
63	76881.0
64	66679.5
65	56478.0
66	48073.5
67	39669.0
68	31490.5
69	23312.0
70	21269.5
71	19227.0
72	15366.5
73	11506.0
74	8840.5
75	4634.5
76	3094.0
77	2548.0
78	2002.0
79	1251.5
80	501.0
81	372.0
82	243.0
83	164.5
84	86.0
85	104.5
86	123.0
87	65.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2278566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.072586931169948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87474719899447	12.677212310298922
2	8.540277801821006	2.7452871477221823
3	2.9245502114402	1.410152625238322
4	1.3680087300054316	0.8794975694244679
5	0.8137959322491511	0.6539902932653485
6	0.506332130696627	0.48828403119996233
7	0.3448010463452008	0.38792913539391194
8	0.2679450041506169	0.34452554975867866
9	0.2037197487575958	0.2946873034352303
>10	2.7218046583576405	11.121445668406183
>50	1.4999471195128844	17.568347302022268
>100	1.9218708965731757	49.64729758737389
>500	0.009521577440806627	0.9950712444887448
>1k	0.0026779436552268636	0.7862722319719063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3415	0.14987496521935287	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2471	0.10844539943104567	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2445	0.10730433088179145	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05358633456305413	0.0
2	0.0	0.0	0.0	0.18112268856816086	0.0
3	0.0	0.0	0.0	0.25963698220723036	0.0
4	0.0	0.0	0.0	0.4289540000157994	0.0
5	0.0	0.0	0.0	0.7128167452687348	0.0
6	0.0	0.0	0.0	1.0026042695274133	0.0
7	0.0	0.0	0.0	1.1668742533681271	0.0
8	0.0	0.0	0.0	1.5563297266789726	0.0
9	0.0	0.0	0.0	1.6774585419074979	0.0
10	4.388725189439323E-5	0.0	0.0	1.8707379992504058	0.0
11	4.388725189439323E-5	0.0	0.0	2.140776260156607	0.0
12	4.388725189439323E-5	0.0	0.0	2.365303440848323	0.0
13	8.777450378878646E-5	0.0	0.0	2.460845988222417	0.0
14	8.777450378878646E-5	0.0	0.0	2.4971846327909746	0.0
15	8.777450378878646E-5	0.0	0.0	2.5573979423900823	0.0
16	8.777450378878646E-5	0.0	0.0	2.688489163798635	0.0
17	8.777450378878646E-5	0.0	0.0	2.844947216802147	0.0
18	8.777450378878646E-5	0.0	0.0	3.0302830815521693	0.0
19	8.777450378878646E-5	0.0	0.0	3.1372802016706998	0.0
20	1.3166175568317967E-4	0.0	0.0	3.2516064928555943	0.0
21	1.3166175568317967E-4	0.0	0.0	3.4055629725011256	0.0
22	1.3166175568317967E-4	0.0	0.0	3.5664975251978657	0.0
23	1.3166175568317967E-4	0.0	0.0	3.7367800625481116	0.0
24	1.3166175568317967E-4	0.0	0.0	3.8687051417426574	0.0
25	1.3166175568317967E-4	0.0	0.0	3.976536119647182	0.0
26	1.3166175568317967E-4	0.0	0.0	4.080856117400154	0.0
27	1.3166175568317967E-4	0.0	0.0	4.180743502711794	0.0
28	1.3166175568317967E-4	0.0	0.0	4.284624627945822	0.0
29	1.3166175568317967E-4	0.0	0.0	4.399345904397766	0.0
30	1.3166175568317967E-4	0.0	0.0	4.54373496313032	0.0
31	1.3166175568317967E-4	0.0	0.0	4.689879511938649	0.0
32	1.3166175568317967E-4	0.0	0.0	4.819303017775215	0.0
33	1.3166175568317967E-4	0.0	0.0	4.950306464679978	0.0
34	1.3166175568317967E-4	0.0	0.0	5.088858518910578	0.0
35	1.7554900757757292E-4	0.0	0.0	5.265065835266567	0.0
36	1.7554900757757292E-4	0.0	0.0	5.424903206665947	0.0
37	1.7554900757757292E-4	0.0	0.0	5.579693544097472	0.0
38	1.7554900757757292E-4	0.0	0.0	5.724828686112231	0.0
39	1.7554900757757292E-4	0.0	0.0	5.872289852477391	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGA	25	3.8920163E-5	45.0	16
GTCGATC	35	1.2126293E-7	45.0	10
CGACGAA	25	3.8920163E-5	45.0	10
CGCGAAA	20	7.0347486E-4	45.0	26
CGTTCGC	20	7.0347486E-4	45.0	41
TAGTTCG	25	3.8920163E-5	45.0	1
CGTTACA	25	3.8920163E-5	45.0	31
GGGCGTA	20	7.0347486E-4	45.0	7
CGCTAAC	20	7.0347486E-4	45.0	25
GAACCGT	25	3.8920163E-5	45.0	18
CAATCGA	25	3.8920163E-5	45.0	22
TCGACAC	20	7.0347486E-4	45.0	34
TATCGTC	25	3.8920163E-5	45.0	33
TCGAATC	45	3.8562575E-10	45.0	17
TACCGTA	20	7.0347486E-4	45.0	45
TCTAGCG	60	0.0	44.999996	1
TACGTAG	140	0.0	43.392857	1
CGGGATA	190	0.0	41.44737	6
TGCGTAG	115	0.0	41.08696	1
CGTTGAT	370	0.0	40.743244	25
>>END_MODULE