FastQCFastQC Report
Thu 26 May 2016
SRR1545283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545283_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2604469
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.1123069616109848No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26860.10313042696995049No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26500.10174818744243068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.1665655E-645.00000443
TACGACA302.1665655E-645.00000423
ATTTCGC600.045.00000427
CGTATCA207.034935E-445.041
CGTTACA207.034935E-445.034
CCCCCGT207.034935E-445.041
CTATTCG453.8562575E-1045.026
CGTCGTA207.034935E-445.022
CGGTCGA351.2127202E-745.036
CAATACG453.8562575E-1045.01
CCGTTGA351.2127202E-745.022
AGTCGAC207.034935E-445.037
GTCGAAC253.8921724E-544.99999629
AACGCAT253.8921724E-544.99999633
ACTCGAT502.1827873E-1144.99999631
TTACGTA253.8921724E-544.9999961
CGTATGG1400.043.3928572
CGATCAT1050.042.85714710
CGTTAGG4200.040.7142872
TGACGTT501.0822987E-940.49999628