##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545283_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2604469
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283459315507308	31.0	31.0	33.0	30.0	34.0
2	31.60379908534139	31.0	31.0	34.0	30.0	34.0
3	31.695928421493978	31.0	31.0	34.0	30.0	34.0
4	30.29391826126554	35.0	33.0	37.0	0.0	37.0
5	32.86911919473797	35.0	33.0	37.0	19.0	37.0
6	34.494420551751624	35.0	33.0	37.0	30.0	37.0
7	35.50130487250952	36.0	35.0	37.0	33.0	37.0
8	35.6867779958218	37.0	35.0	37.0	35.0	37.0
9	37.69675354170082	39.0	37.0	39.0	35.0	39.0
10	37.161714729566754	39.0	37.0	39.0	33.0	39.0
11	36.72527183084153	39.0	35.0	39.0	32.0	39.0
12	35.65291927068435	37.0	35.0	39.0	31.0	39.0
13	35.21370651752814	37.0	35.0	39.0	30.0	39.0
14	36.06557075549757	38.0	35.0	40.0	30.0	41.0
15	36.3564834137016	38.0	35.0	40.0	31.0	41.0
16	36.520685022551625	38.0	35.0	40.0	32.0	41.0
17	36.460087641665154	38.0	35.0	40.0	31.0	41.0
18	36.38949436526217	37.0	35.0	40.0	31.0	41.0
19	36.4017394716543	37.0	35.0	40.0	31.0	41.0
20	36.32486660428671	37.0	35.0	40.0	31.0	41.0
21	36.177272219404415	37.0	34.0	40.0	31.0	41.0
22	36.03819051023452	37.0	34.0	40.0	30.0	41.0
23	35.993734231430665	37.0	34.0	40.0	30.0	41.0
24	35.93821926849581	37.0	34.0	40.0	30.0	41.0
25	35.823018050896366	36.0	34.0	40.0	30.0	41.0
26	35.64252943690249	36.0	34.0	40.0	30.0	41.0
27	35.63029623312852	36.0	34.0	40.0	30.0	41.0
28	35.650975304371066	36.0	34.0	40.0	30.0	41.0
29	35.6737753453775	36.0	34.0	40.0	30.0	41.0
30	35.63577105352377	36.0	34.0	40.0	30.0	41.0
31	35.51825343284946	36.0	34.0	40.0	30.0	41.0
32	35.43525801228581	36.0	34.0	40.0	29.0	41.0
33	35.30305832014127	36.0	34.0	40.0	29.0	41.0
34	35.21272090395394	36.0	34.0	40.0	29.0	41.0
35	35.028288683796966	36.0	34.0	40.0	27.0	41.0
36	34.920314659149334	36.0	34.0	40.0	27.0	41.0
37	34.895036185878965	35.0	33.0	40.0	27.0	41.0
38	34.90971019428528	35.0	33.0	40.0	27.0	41.0
39	34.84085969155325	35.0	34.0	40.0	27.0	41.0
40	34.71271687242198	35.0	33.0	40.0	27.0	41.0
41	34.662792300465085	35.0	34.0	40.0	27.0	41.0
42	34.63613350744432	35.0	33.0	40.0	27.0	41.0
43	34.58486432359149	35.0	33.0	40.0	27.0	41.0
44	34.50568273225752	35.0	33.0	40.0	27.0	41.0
45	34.39465472616491	35.0	33.0	39.0	27.0	41.0
46	34.31952079291403	35.0	33.0	39.0	26.0	41.0
47	34.2545778045352	35.0	33.0	39.0	26.0	41.0
48	34.18159095001707	35.0	33.0	39.0	26.0	41.0
49	34.1357493600423	35.0	33.0	39.0	26.0	41.0
50	34.006030019938805	35.0	33.0	39.0	26.0	41.0
51	33.91555437979872	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	7.0
13	16.0
14	21.0
15	49.0
16	165.0
17	482.0
18	1113.0
19	2224.0
20	3950.0
21	6406.0
22	9552.0
23	14471.0
24	20139.0
25	27688.0
26	35861.0
27	44620.0
28	53604.0
29	65660.0
30	81417.0
31	101913.0
32	130647.0
33	190509.0
34	287675.0
35	383864.0
36	194878.0
37	244644.0
38	316559.0
39	386110.0
40	221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.4816306126124	24.89309721098619	30.31247444296707	12.312797733434339
2	28.25616277252676	26.735699292254967	31.426597897690467	13.581540037527803
3	29.90767023911592	25.92175218825795	30.275653117775637	13.894924454850491
4	22.590938882359513	39.08117163229818	25.091026232218542	13.236863253123765
5	24.250509412859206	32.53311903501251	29.137071702523627	14.07929984960466
6	22.39512161596087	41.29225573427827	25.490915806638515	10.82170684312234
7	89.52128053741473	4.287898992078616	4.285864028329767	1.9049564421768888
8	91.78823015363209	2.7784550324845485	3.5723212677900946	1.8609935460932727
9	87.62131551575388	4.788308096583219	5.086449483560757	2.5039269041021415
10	58.827499962564346	22.461776277621272	9.190817782818685	9.519905976995695
11	51.77723367027981	20.014597985232307	15.057387897494653	13.150780446993227
12	47.81688705068096	20.203696031705505	18.111945275601286	13.86747164201225
13	24.327722848688158	38.613129970063	19.505549883680704	17.553597297568142
14	15.403830876850522	41.15195842223501	23.954633362885104	19.489577338029367
15	15.773963905886381	23.350901853698396	46.02323928601185	14.851894954403374
16	19.51061809528161	17.50902007280563	43.88522190127815	19.095139930634613
17	19.247263069746655	19.319945831568738	25.46576672634614	35.96702437233847
18	22.475253113014592	25.252095532717036	30.147066446173866	22.12558490809451
19	33.98132210442896	25.21446790113455	20.35804611227855	20.446163882157936
20	36.495922969326955	24.472742812450445	20.49112506234476	18.54020915587784
21	27.743505489986635	28.421954724744275	22.90501441944596	20.929525365823128
22	24.675317694316963	27.08475316849615	21.885843141154684	26.354085996032207
23	24.67520250768967	30.296617083943023	22.64784107624241	22.380339332124898
24	25.250098964510613	22.084156117811347	33.94787958697147	18.717865330706566
25	22.479131062800136	24.17152978207842	30.72906607834457	22.62027307677688
26	19.598543887448844	35.73619037124266	22.80618429322829	21.859081448080204
27	21.58224958715193	32.66996074823697	26.45990411097233	19.287885553638766
28	18.445333770530574	28.986714758363412	34.04705527307102	18.520896198034993
29	19.314301686831364	26.537847062107478	32.39316728285113	21.754683968210024
30	22.60718019680787	29.59455459059025	26.739001308904044	21.059263903697836
31	31.11302150265563	26.387720491201854	21.330221246634153	21.16903675950837
32	35.40982826057826	26.44988287439781	21.332524979180018	16.80776388584391
33	31.025172501573255	27.3039149246929	21.76520434683615	19.90570822689769
34	22.836708749461025	26.565607039285165	25.54969938210054	25.047984829153275
35	23.143335551315836	24.446403470342705	28.770202294594405	23.64005868374705
36	30.214373832055593	27.776564052019815	21.646600516266464	20.362461599658126
37	23.938776003861058	33.52572059794146	24.294587495570113	18.240915902627368
38	23.532589560482386	32.25037426054985	21.2340020173018	22.983034161665966
39	23.87058552050341	32.1340357669836	23.39770601992191	20.597672692591082
40	28.104922730890635	24.024474854567284	23.109777847231047	24.760824567311033
41	17.79652589452975	24.906766024091667	25.88374060125116	31.412967480127428
42	22.80568515117669	27.467710308704003	21.668601162079486	28.058003378039825
43	23.065622973435275	27.247742246116196	22.818816426688127	26.867818353760402
44	21.504460218186512	29.811758174123014	25.73449712782145	22.949284479869025
45	19.769058491385383	33.30329522063806	20.93866350492173	25.98898278305482
46	22.40506606145053	32.76061262391681	22.954890229063967	21.87943108556869
47	22.9709779613426	26.67856672511748	25.288724880196305	25.06173043334361
48	24.736174629070263	21.3017701496927	29.163526231258658	24.79852898997838
49	21.015070634359635	23.750676241491067	30.4101527029118	24.824100421237496
50	20.190641547278926	31.670371196585563	24.284758236707752	23.854229019427763
51	18.25235777427184	33.12529348592746	22.440735520369024	26.181613219431675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	369.0
1	966.0
2	1563.0
3	4240.0
4	6917.0
5	5118.0
6	3319.0
7	3520.0
8	3721.0
9	3934.0
10	4147.0
11	4195.5
12	4244.0
13	4200.5
14	4157.0
15	3983.0
16	3809.0
17	4159.0
18	4509.0
19	4578.0
20	4647.0
21	4672.0
22	4697.0
23	5423.0
24	6149.0
25	8713.5
26	13637.5
27	15997.0
28	21775.0
29	27553.0
30	31754.5
31	35956.0
32	39515.5
33	43075.0
34	48398.0
35	53721.0
36	55813.0
37	57905.0
38	76292.5
39	94680.0
40	134115.0
41	173550.0
42	204037.5
43	234525.0
44	236730.5
45	238936.0
46	221533.0
47	204130.0
48	200017.0
49	195904.0
50	180937.5
51	165971.0
52	152015.5
53	138060.0
54	130702.5
55	123345.0
56	129634.5
57	135924.0
58	141902.5
59	147881.0
60	147145.0
61	146409.0
62	127815.0
63	109221.0
64	90702.5
65	72184.0
66	58922.5
67	45661.0
68	38625.0
69	31589.0
70	24956.0
71	18323.0
72	15744.0
73	13165.0
74	11445.5
75	6810.5
76	3895.0
77	2942.0
78	1989.0
79	1456.5
80	924.0
81	642.0
82	360.0
83	301.0
84	242.0
85	166.0
86	90.0
87	60.0
88	30.0
89	22.5
90	15.0
91	8.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2604469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.788253961862203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51393368814975	12.395979246133166
2	8.892115868047005	2.807819671660618
3	2.997221269403401	1.4196267174370771
4	1.4462729609874105	0.9133649922497464
5	0.8216632397308932	0.6486313949998903
6	0.5080891652878772	0.48131044661013517
7	0.36788225876818925	0.40657529706469764
8	0.26657123883610373	0.3366955534138104
9	0.20591421284838732	0.2925923298126559
>10	2.6194602765499946	10.322766090962139
>50	1.396358385541914	16.044696554757152
>100	1.9526144757574602	52.14309043785067
>500	0.008728837400598632	0.9456066852028298
>1k	0.003174122691126775	0.8412445818454181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2925	0.1123069616109848	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2686	0.10313042696995049	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2650	0.10174818744243068	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059858650650094124	0.0
2	0.0	0.0	0.0	0.20077029137225286	0.0
3	0.0	0.0	0.0	0.2828983566323884	0.0
4	0.0	0.0	0.0	0.4605161359186844	0.0
5	0.0	0.0	0.0	0.752207071767796	0.0
6	0.0	0.0	0.0	1.0537272664792707	0.0
7	0.0	0.0	0.0	1.2189432855603195	0.0
8	0.0	0.0	0.0	1.622250063256656	0.0
9	0.0	0.0	0.0	1.7398556097231337	0.0
10	0.0	0.0	0.0	1.9365559735976892	0.0
11	0.0	0.0	0.0	2.2436435219616744	0.0
12	0.0	0.0	0.0	2.487532007484059	0.0
13	3.839554243110592E-5	0.0	0.0	2.5851334763439304	0.0
14	3.839554243110592E-5	0.0	0.0	2.6231834588931564	0.0
15	7.679108486221184E-5	0.0	0.0	2.687649574634983	0.0
16	7.679108486221184E-5	0.0	0.0	2.8326695383972704	0.0
17	7.679108486221184E-5	0.0	0.0	2.9984998861572167	0.0
18	7.679108486221184E-5	0.0	0.0	3.187444350460689	0.0
19	7.679108486221184E-5	0.0	0.0	3.3097341531037614	0.0
20	7.679108486221184E-5	0.0	0.0	3.4370921673477395	0.0
21	7.679108486221184E-5	0.0	0.0	3.607990726708592	0.0
22	7.679108486221184E-5	0.0	0.0	3.776931113405458	0.0
23	7.679108486221184E-5	0.0	0.0	3.9589643800713312	0.0
24	7.679108486221184E-5	0.0	0.0	4.10578893432788	0.0
25	7.679108486221184E-5	0.0	0.0	4.228309110225539	0.0
26	7.679108486221184E-5	0.0	0.0	4.346298612116327	0.0
27	7.679108486221184E-5	0.0	0.0	4.4542284818901665	0.0
28	7.679108486221184E-5	0.0	0.0	4.5702214155745375	0.0
29	7.679108486221184E-5	0.0	0.0	4.696504354630445	0.0
30	7.679108486221184E-5	0.0	0.0	4.85745846850164	0.0
31	7.679108486221184E-5	0.0	0.0	5.012384482211154	0.0
32	7.679108486221184E-5	0.0	0.0	5.164622807950488	0.0
33	7.679108486221184E-5	0.0	0.0	5.307684599048788	0.0
34	7.679108486221184E-5	0.0	0.0	5.463186545894768	0.0
35	7.679108486221184E-5	0.0	0.0	5.646256492206281	0.0
36	7.679108486221184E-5	0.0	0.0	5.818268522297635	0.0
37	7.679108486221184E-5	0.0	0.0	6.0014920507788725	0.0
38	7.679108486221184E-5	0.0	0.0	6.1714691171213785	0.0
39	7.679108486221184E-5	0.0	0.0	6.354539063432892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.1665655E-6	45.000004	43
TACGACA	30	2.1665655E-6	45.000004	23
ATTTCGC	60	0.0	45.000004	27
CGTATCA	20	7.034935E-4	45.0	41
CGTTACA	20	7.034935E-4	45.0	34
CCCCCGT	20	7.034935E-4	45.0	41
CTATTCG	45	3.8562575E-10	45.0	26
CGTCGTA	20	7.034935E-4	45.0	22
CGGTCGA	35	1.2127202E-7	45.0	36
CAATACG	45	3.8562575E-10	45.0	1
CCGTTGA	35	1.2127202E-7	45.0	22
AGTCGAC	20	7.034935E-4	45.0	37
GTCGAAC	25	3.8921724E-5	44.999996	29
AACGCAT	25	3.8921724E-5	44.999996	33
ACTCGAT	50	2.1827873E-11	44.999996	31
TTACGTA	25	3.8921724E-5	44.999996	1
CGTATGG	140	0.0	43.392857	2
CGATCAT	105	0.0	42.857147	10
CGTTAGG	420	0.0	40.714287	2
TGACGTT	50	1.0822987E-9	40.499996	28
>>END_MODULE