FastQCFastQC Report
Thu 26 May 2016
SRR1545282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545282_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3477018
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.1280695124385321No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT207.03526E-445.022
TTACGAT207.03526E-445.043
TCGAGTA207.03526E-445.01
CGTAAGG4550.041.538462
CTAAGCG1850.041.3513531
CCCGTAT603.6379788E-1241.24999634
ATATGCG1150.041.086961
ACGTAGG4750.040.2631572
AATAGCG2650.039.9056551
CGTTGAT6450.039.41860625
TAAGTCG403.4609184E-739.3751
GTCGGTA403.4609184E-739.37529
CGTTTTT37750.039.218541
CGACCCG9500.039.07894532
ATAGGGC19800.038.6363644
CGCTAAG356.251941E-638.57142630
GCGTAAG2800.038.5714261
CTCGACA659.094947E-1238.07692731
TACGGGA8750.037.542864
CGAATAA900.037.512
Produced by FastQC (version 0.11.5)