##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545282_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3477018
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217394905634656	31.0	31.0	33.0	30.0	34.0
2	31.538036904036733	31.0	31.0	34.0	30.0	34.0
3	31.633512394816478	31.0	31.0	34.0	30.0	34.0
4	30.29944768764499	35.0	33.0	37.0	0.0	37.0
5	32.83189416908397	35.0	33.0	37.0	19.0	37.0
6	34.43402996475716	35.0	33.0	37.0	30.0	37.0
7	35.46025473552337	36.0	35.0	37.0	33.0	37.0
8	35.65109844125052	37.0	35.0	37.0	35.0	37.0
9	37.657543906876526	39.0	37.0	39.0	35.0	39.0
10	37.078701059356035	39.0	37.0	39.0	33.0	39.0
11	36.633091056761856	39.0	35.0	39.0	32.0	39.0
12	35.62871949469344	37.0	35.0	39.0	31.0	39.0
13	35.20395781672686	37.0	35.0	39.0	30.0	39.0
14	35.9567764676513	38.0	35.0	40.0	30.0	41.0
15	36.25473552337089	38.0	35.0	40.0	30.0	41.0
16	36.42114104672452	38.0	35.0	40.0	31.0	41.0
17	36.35639217283316	38.0	35.0	40.0	31.0	41.0
18	36.32940352911604	38.0	35.0	40.0	31.0	41.0
19	36.33461345325218	37.0	35.0	40.0	31.0	41.0
20	36.2288466726373	37.0	35.0	40.0	30.0	41.0
21	36.082646969328316	37.0	34.0	40.0	30.0	41.0
22	36.00290593836443	37.0	34.0	40.0	30.0	41.0
23	35.938858240020615	37.0	34.0	40.0	30.0	41.0
24	35.87918095333415	37.0	34.0	40.0	30.0	41.0
25	35.757270741767805	37.0	34.0	40.0	30.0	41.0
26	35.6090471777828	36.0	34.0	40.0	30.0	41.0
27	35.61192895751474	37.0	34.0	40.0	30.0	41.0
28	35.61742188277426	36.0	34.0	40.0	29.0	41.0
29	35.626831094920995	36.0	34.0	40.0	30.0	41.0
30	35.551184376957494	36.0	34.0	40.0	29.0	41.0
31	35.43618583510353	36.0	34.0	40.0	29.0	41.0
32	35.36419627393359	36.0	34.0	40.0	29.0	41.0
33	35.22431635384114	36.0	34.0	40.0	29.0	41.0
34	35.0817551131458	36.0	34.0	40.0	27.0	41.0
35	34.96998491235881	36.0	34.0	40.0	27.0	41.0
36	34.82825398085371	36.0	33.0	40.0	27.0	41.0
37	34.77197961011419	36.0	33.0	40.0	27.0	41.0
38	34.72374948878608	35.0	33.0	40.0	27.0	41.0
39	34.650514032426635	35.0	33.0	40.0	26.0	41.0
40	34.50619438840984	35.0	33.0	40.0	26.0	41.0
41	34.45790272008946	35.0	33.0	40.0	26.0	41.0
42	34.422048721059255	35.0	33.0	40.0	26.0	41.0
43	34.332010360602105	35.0	33.0	40.0	26.0	41.0
44	34.27502934986244	35.0	33.0	40.0	26.0	41.0
45	34.20457645028009	35.0	33.0	39.0	26.0	41.0
46	34.13992967537125	35.0	33.0	39.0	26.0	41.0
47	34.0872037475791	35.0	33.0	39.0	25.0	41.0
48	33.97035735794293	35.0	33.0	39.0	25.0	41.0
49	33.917119784827115	35.0	33.0	39.0	24.0	41.0
50	33.80571023791076	35.0	33.0	39.0	24.0	40.0
51	33.71202018511264	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	6.0
12	3.0
13	12.0
14	30.0
15	89.0
16	341.0
17	811.0
18	1855.0
19	3585.0
20	6165.0
21	9998.0
22	15279.0
23	21371.0
24	29742.0
25	39951.0
26	51839.0
27	64051.0
28	76144.0
29	92291.0
30	113504.0
31	141330.0
32	178387.0
33	253233.0
34	370280.0
35	472253.0
36	269413.0
37	337154.0
38	428080.0
39	499561.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22258268435769	24.924547413904673	30.789803216434315	12.063066685303326
2	28.48906735599298	26.90351329788917	31.10383667844112	13.503582667676728
3	28.799707105341415	26.400381016146596	30.841226591291733	13.958685287220257
4	23.063584945490646	38.7847575134785	25.843553297682092	12.308104243348755
5	24.117994212281904	32.33256198271047	28.87528911268219	14.674154692325436
6	22.33802068324064	40.89231059488332	25.665498424224438	11.104170297651608
7	89.9557609422787	4.1638553496128	4.1889918315061925	1.6913918766023068
8	92.11255161750672	2.4676892670673545	3.611054069895525	1.8087050455303941
9	87.95531113155009	4.5728840057773645	4.934458205278201	2.5373466573943535
10	57.26478839051165	23.76427156833816	9.176340185756876	9.794599855393328
11	48.51102295127607	20.600439802152305	17.615554478003855	13.272982768567779
12	44.189590045262925	20.277001729643047	20.453589828985642	15.079818396108388
13	24.73708792994457	35.10692208093257	22.025137632304464	18.1308523568184
14	17.74592481258366	37.10193619935243	26.939693726060664	18.212445262003246
15	17.528468359956722	22.478600916072335	42.964517296142844	17.0284134278281
16	21.498968368872408	19.04560171963447	40.046959779903354	19.40847013158977
17	21.42229347101453	20.441855636065156	25.605331925230185	32.53051896769013
18	25.49555394881476	24.60939230110399	28.47759200556339	21.41746174451786
19	33.356053951978396	25.463256158006658	21.23549547342004	19.945194416594912
20	34.53372976498827	25.333259707024812	21.68714685975166	18.44586366823525
21	26.74541805650704	27.05252029181327	24.810973080956153	21.391088570723536
22	25.472315645187916	24.877840724436858	24.09642400470748	25.55341962566774
23	24.640280838350563	28.78943968653599	23.934417365685192	22.635862109428253
24	24.28946298236017	23.10609263455064	32.74486931042635	19.85957507266284
25	22.64141284284407	24.56973187944382	29.781640474682614	23.007214803029495
26	20.324082302708817	33.123181991004934	23.84126282924046	22.71147287704579
27	21.193189106297407	30.838408084168677	27.156402411491687	20.81200039804223
28	18.54517290390789	28.07632862412562	33.78000919178446	19.59848928018204
29	20.493940497288193	25.923765709582174	31.59727674691359	21.98501704621604
30	23.542155950875145	27.920764287098887	27.10929307814915	21.427786683876814
31	31.021438485506835	25.287415825859977	22.41003641626244	21.28110927237075
32	34.07595243970552	25.226185196625384	21.595200254931093	19.102662108738006
33	30.41074276865981	26.347979791879133	22.16824876949156	21.073028669969496
34	22.26571734745118	26.217408135361968	27.23917448802393	24.277700029162922
35	22.929360733824215	25.84792485975051	27.911676039640867	23.311038366784413
36	30.85468640081817	26.569060039378574	23.046990265796726	19.52926329400653
37	24.478533041819166	31.709355545470284	24.54804663076234	19.26406478194821
38	23.713711001783714	32.473084694988636	21.013840020385285	22.799364282842365
39	25.14220518846897	29.832114760406764	23.15331700900024	21.872363042124025
40	27.204978518949286	24.35529525587731	23.611123094559762	24.828603130613647
41	19.986695495968096	23.520729544684553	25.730726731929487	30.76184822741786
42	23.326223792916803	25.329520871045247	22.586308152560612	28.757947183477334
43	22.555563416697872	26.081659629026944	24.913014542921548	26.44976241135364
44	22.509777056086566	28.412076095090676	26.303832767043485	22.77431408177927
45	20.717925532740985	31.709671908514707	22.226488329942494	25.345914228801806
46	21.860024883391457	31.0066269429724	23.971748204927326	23.161599968708817
47	22.82939001178596	25.004961147742115	27.32332705784094	24.84232178263098
48	24.386298834230942	22.576011973478423	29.662630449425343	23.375058742865296
49	22.61555735403153	23.604019306198587	29.725414133605288	24.055009206164595
50	19.880886437746366	30.813185321444987	24.906112076497735	24.399816164310913
51	18.95687626581168	31.885828603705818	24.15167249637477	25.005622634107734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503.0
1	1156.0
2	1809.0
3	5684.0
4	9559.0
5	7043.0
6	4527.0
7	4787.5
8	5048.0
9	5375.0
10	5702.0
11	5612.0
12	5522.0
13	5396.5
14	5271.0
15	5372.0
16	5473.0
17	5679.5
18	5886.0
19	5729.0
20	5572.0
21	5698.5
22	5825.0
23	7591.0
24	9357.0
25	10823.0
26	17932.0
27	23575.0
28	28740.0
29	33905.0
30	39898.0
31	45891.0
32	51334.5
33	56778.0
34	62820.0
35	68862.0
36	79966.5
37	91071.0
38	110564.5
39	130058.0
40	179266.0
41	228474.0
42	260609.0
43	292744.0
44	294517.0
45	296290.0
46	278540.5
47	260791.0
48	252295.5
49	243800.0
50	233470.5
51	223141.0
52	215682.0
53	208223.0
54	199067.5
55	189912.0
56	190084.5
57	190257.0
58	195081.5
59	199906.0
60	193540.5
61	187175.0
62	166190.0
63	145205.0
64	121840.5
65	98476.0
66	81994.5
67	65513.0
68	53445.5
69	41378.0
70	35360.0
71	29342.0
72	23756.0
73	18170.0
74	15750.5
75	9417.0
76	5503.0
77	4428.5
78	3354.0
79	2692.5
80	2031.0
81	1385.0
82	739.0
83	541.0
84	343.0
85	358.0
86	373.0
87	206.0
88	39.0
89	25.0
90	11.0
91	10.5
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3477018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.42496905468457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83568028836369	12.784486400866957
2	9.047962578926851	2.972250107336351
3	3.1470643731263697	1.5507130482510276
4	1.592206605455021	1.0460777689305236
5	0.9548779170522236	0.7841920119292217
6	0.6505274095770904	0.6410935542916696
7	0.4575489934606009	0.526065964101471
8	0.36383358721726766	0.4780764328878801
9	0.2677204673740934	0.3957570352732657
>10	2.6679061283672043	10.361380817273519
>50	1.2242330661539633	14.76457745064755
>100	1.7762985209786741	51.83864668243439
>500	0.012038162549511844	1.2433541171836524
>1k	0.0021019013975338142	0.6133286085925619
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4453	0.1280695124385321	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05320651201690644	0.0
2	0.0	0.0	0.0	0.17998181200097324	0.0
3	0.0	0.0	0.0	0.26281140908675193	0.0
4	0.0	0.0	0.0	0.4253644933675926	0.0
5	0.0	0.0	0.0	0.7290154954619159	0.0
6	0.0	0.0	0.0	1.032695257833005	0.0
7	2.8760276765895374E-5	0.0	0.0	1.205630802026334	0.0
8	2.8760276765895374E-5	0.0	0.0	1.619347383303739	0.0
9	2.8760276765895374E-5	0.0	0.0	1.7448572311101065	0.0
10	5.752055353179075E-5	0.0	0.0	1.9582009641595184	0.0
11	5.752055353179075E-5	0.0	0.0	2.2987801616212513	0.0
12	5.752055353179075E-5	0.0	0.0	2.557306289469885	0.0
13	5.752055353179075E-5	0.0	0.0	2.6674006289297325	0.0
14	5.752055353179075E-5	0.0	0.0	2.712065338747168	0.0
15	5.752055353179075E-5	0.0	0.0	2.7775812492198777	0.0
16	5.752055353179075E-5	0.0	0.0	2.9294067502670393	0.0
17	5.752055353179075E-5	0.0	0.0	3.103147582209813	0.0
18	5.752055353179075E-5	0.0	0.0	3.291211031982003	0.0
19	5.752055353179075E-5	0.0	0.0	3.4221278118203586	0.0
20	5.752055353179075E-5	0.0	0.0	3.561327551367292	0.0
21	5.752055353179075E-5	0.0	0.0	3.7500812477818637	0.0
22	5.752055353179075E-5	0.0	0.0	3.9480669930382875	0.0
23	5.752055353179075E-5	0.0	0.0	4.15243751973674	0.0
24	5.752055353179075E-5	0.0	0.0	4.310561521395632	0.0
25	5.752055353179075E-5	0.0	0.0	4.445188376936789	0.0
26	5.752055353179075E-5	0.0	0.0	4.577859533657865	0.0
27	8.628083029768612E-5	0.0	0.0	4.714442088019101	0.0
28	8.628083029768612E-5	0.0	0.0	4.857495704652665	0.0
29	8.628083029768612E-5	0.0	0.0	5.013175082786456	0.0
30	8.628083029768612E-5	0.0	0.0	5.195515237482233	0.0
31	8.628083029768612E-5	0.0	0.0	5.373282508172233	0.0
32	8.628083029768612E-5	0.0	0.0	5.527782714958622	0.0
33	1.150411070635815E-4	0.0	0.0	5.691773813077758	0.0
34	1.150411070635815E-4	0.0	0.0	5.8686495151880145	0.0
35	1.150411070635815E-4	0.0	0.0	6.076615076482204	0.0
36	1.4380138382947687E-4	0.0	0.0	6.261457375256613	0.0
37	1.4380138382947687E-4	0.0	0.0	6.466805751365107	0.0
38	1.4380138382947687E-4	0.0	0.0	6.709024802287478	0.0
39	1.4380138382947687E-4	0.0	0.0	7.096713333091747	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGT	20	7.03526E-4	45.0	22
TTACGAT	20	7.03526E-4	45.0	43
TCGAGTA	20	7.03526E-4	45.0	1
CGTAAGG	455	0.0	41.53846	2
CTAAGCG	185	0.0	41.351353	1
CCCGTAT	60	3.6379788E-12	41.249996	34
ATATGCG	115	0.0	41.08696	1
ACGTAGG	475	0.0	40.263157	2
AATAGCG	265	0.0	39.905655	1
CGTTGAT	645	0.0	39.418606	25
TAAGTCG	40	3.4609184E-7	39.375	1
GTCGGTA	40	3.4609184E-7	39.375	29
CGTTTTT	3775	0.0	39.21854	1
CGACCCG	950	0.0	39.078945	32
ATAGGGC	1980	0.0	38.636364	4
CGCTAAG	35	6.251941E-6	38.571426	30
GCGTAAG	280	0.0	38.571426	1
CTCGACA	65	9.094947E-12	38.076927	31
TACGGGA	875	0.0	37.54286	4
CGAATAA	90	0.0	37.5	12
>>END_MODULE