FastQCFastQC Report
Thu 26 May 2016
SRR1545281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545281_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3377849
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.11607978923865454No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35380.10474121252903845No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA207.0352305E-445.00000416
TACCCGC207.0352305E-445.00000423
TCGCACC700.045.00000438
GACACGT207.0352305E-445.00000415
CGCGAAC207.0352305E-445.00000435
CCCGTTG207.0352305E-445.00000430
TACGAAC207.0352305E-445.00000445
TGGTCGA700.045.00000412
GTACCTA551.8189894E-1245.043
CTATCGT253.892418E-545.013
CTATACG502.1827873E-1145.01
CGGTTCA502.1827873E-1145.041
CACGCGA502.1827873E-1145.037
TATGATC551.8189894E-1245.035
CGTAAGA253.892418E-545.043
CCGCGTA551.8189894E-1245.036
CACGTAC253.892418E-545.030
CAATACG253.892418E-545.01
AATCGTC253.892418E-545.023
TACGTCA453.8562575E-1045.015