##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545281_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3377849
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340452755584987	31.0	31.0	33.0	30.0	34.0
2	31.6582683240133	31.0	31.0	34.0	30.0	34.0
3	31.73734497900883	31.0	31.0	34.0	30.0	34.0
4	30.285251945838905	35.0	33.0	37.0	0.0	37.0
5	32.87983743500671	35.0	33.0	37.0	19.0	37.0
6	34.52031692358066	35.0	33.0	37.0	30.0	37.0
7	35.51624095689298	36.0	35.0	37.0	33.0	37.0
8	35.72180639217443	37.0	35.0	37.0	35.0	37.0
9	37.77876246096258	39.0	37.0	39.0	35.0	39.0
10	37.238089979747464	39.0	37.0	39.0	34.0	39.0
11	36.759636976075605	39.0	35.0	39.0	32.0	39.0
12	35.47167472554279	37.0	35.0	39.0	31.0	39.0
13	34.971511159912716	35.0	34.0	39.0	30.0	39.0
14	35.74914716436407	37.0	34.0	40.0	30.0	41.0
15	36.13608897259765	37.0	35.0	40.0	31.0	41.0
16	36.33926560956396	37.0	35.0	40.0	32.0	41.0
17	36.29578350009133	36.0	35.0	40.0	32.0	41.0
18	36.2905070652951	36.0	35.0	40.0	31.0	41.0
19	36.25443262857517	36.0	35.0	40.0	31.0	41.0
20	36.16391881342239	36.0	35.0	40.0	31.0	41.0
21	35.974061895602794	36.0	34.0	40.0	31.0	41.0
22	35.8395020026058	36.0	34.0	40.0	30.0	41.0
23	35.825779364323274	36.0	34.0	40.0	31.0	41.0
24	35.755466866636134	35.0	34.0	40.0	30.0	41.0
25	35.650357076352435	35.0	34.0	40.0	30.0	41.0
26	35.494567104687036	35.0	34.0	40.0	30.0	41.0
27	35.49283434517055	35.0	34.0	40.0	30.0	41.0
28	35.55372842302897	36.0	34.0	40.0	30.0	41.0
29	35.62678527074478	36.0	34.0	40.0	30.0	41.0
30	35.588620154423715	36.0	34.0	40.0	30.0	41.0
31	35.4408154420165	35.0	34.0	40.0	30.0	41.0
32	35.31700617760001	35.0	34.0	40.0	30.0	41.0
33	35.19021069325479	35.0	34.0	40.0	29.0	41.0
34	35.13362497849963	35.0	34.0	40.0	29.0	41.0
35	35.006425390833044	35.0	34.0	40.0	29.0	41.0
36	34.806279084707455	35.0	33.0	40.0	27.0	41.0
37	34.73928023425559	35.0	33.0	40.0	27.0	41.0
38	34.76533231651267	35.0	33.0	40.0	28.0	41.0
39	34.72319336950823	35.0	34.0	40.0	27.0	41.0
40	34.60572808316772	35.0	33.0	40.0	27.0	41.0
41	34.6187419863943	35.0	34.0	40.0	27.0	41.0
42	34.57658586869928	35.0	33.0	39.0	27.0	41.0
43	34.464485831071784	35.0	33.0	39.0	27.0	41.0
44	34.403042883207625	35.0	33.0	39.0	27.0	41.0
45	34.31511177675497	35.0	33.0	39.0	27.0	41.0
46	34.28205760529852	35.0	33.0	39.0	27.0	41.0
47	34.238170800411744	35.0	33.0	39.0	27.0	41.0
48	34.15581099095904	35.0	33.0	39.0	27.0	41.0
49	34.16040503882797	35.0	33.0	39.0	27.0	41.0
50	33.974677672092504	35.0	33.0	39.0	26.0	40.0
51	33.878114445021076	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	4.0
13	5.0
14	17.0
15	63.0
16	173.0
17	496.0
18	1191.0
19	2442.0
20	4513.0
21	7468.0
22	11636.0
23	17207.0
24	24334.0
25	34293.0
26	45740.0
27	56260.0
28	68734.0
29	84023.0
30	105922.0
31	134950.0
32	175429.0
33	262621.0
34	404787.0
35	567870.0
36	236063.0
37	288899.0
38	373296.0
39	468997.0
40	411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.541759267510184	25.54205353762113	30.818221892097604	12.097965302771083
2	27.956607888629716	26.82701328567381	31.469494343885714	13.746884481810762
3	28.473297651848856	26.35330945817886	31.04019747478351	14.133195415188778
4	22.41556682965994	38.84181915769474	26.105252188596946	12.637361824048382
5	23.99527036288478	32.47424618448012	29.38728166948848	14.143201783146612
6	21.737768621391897	41.589307278093244	25.823327212080827	10.84959688843403
7	90.13567509974543	4.158415607091969	4.018652106710513	1.6872571864520882
8	92.1845825553481	2.4197351628210737	3.439022881129381	1.9566594007014524
9	88.89156975341407	4.341253857114395	4.654589355533655	2.11258703393787
10	61.344571648999114	21.59282430919795	8.231096179846997	8.831507861955936
11	54.65143646148777	17.71210021525533	16.2422002878163	11.3942630354406
12	48.22820676708758	20.675139711692264	18.12801578756185	12.968637733658314
13	21.641020661373556	45.801307281645805	19.023230464120804	13.534441592859835
14	14.461244419155502	46.46735244825923	24.345108381102882	14.726294751482378
15	13.75837108171502	22.069755042336116	50.13027521360487	14.041598662343995
16	16.232815617275964	17.091586983313935	47.62684773653292	19.048749662877174
17	16.628155965527174	19.329697686308652	24.89696845536908	39.14517789279509
18	22.87316573357779	24.89383036364266	31.397999140873377	20.835004761906173
19	33.825076254148726	24.505151059150364	21.700022706758055	19.969749979942858
20	35.88410849626493	24.25815955656988	20.52406723924012	19.33366470792507
21	23.834457964225162	30.267871654416762	24.74258026335695	21.15509011800113
22	23.654136108511658	26.074492968750228	22.308782897044836	27.962588025693275
23	21.635425384616067	31.871022061672978	22.599411637405936	23.89414091630502
24	21.10230504679161	23.208882339026996	38.294873453490666	17.393939160690724
25	18.22514860788626	25.476242425283075	34.63446708245395	21.664141884376715
26	16.576051801013012	38.54873323230257	24.743438797885872	20.131776168798545
27	19.321970875548313	35.2152508889533	27.206663175292917	18.256115060205474
28	15.105678199351125	29.39995245494988	39.037446611734275	16.45692273396472
29	16.206141837601386	25.12308868750498	37.366916046276785	21.30385342861685
30	20.12120731270107	31.70819654756622	29.28692194352086	18.88367419621185
31	33.38985845726082	26.86940712861943	20.105368830874323	19.63536558324543
32	35.428374684599575	26.53644375459057	21.719236117422653	16.315945443387196
33	29.62263854897007	27.822114013977533	21.840111858167727	20.71513557888467
34	19.797125330350767	27.400751188108174	26.946260771277814	25.85586271026325
35	21.643181800015334	25.219955066079038	30.173669693346266	22.963193440559362
36	33.151777950997804	24.099034622329178	24.330246852360776	18.41894057431223
37	21.307080334260057	34.04086446729857	26.58283422379153	18.069220974649845
38	21.840733555585228	34.63952947571073	21.251364403796615	22.26837256490743
39	21.605672722492926	34.08239977571525	24.42743888196305	19.884488619828772
40	26.638402131060328	25.539389120117566	22.508288558784006	25.313920190038097
41	17.887211654517415	23.06322751549877	25.630630617295207	33.41893021268861
42	23.268802128218283	25.786587855170556	21.763939122204693	29.180670894406468
43	22.27334614424742	26.92601119825072	24.303010584546556	26.4976320729553
44	20.598611720062088	31.47793166598033	27.20254813048185	20.72090848347573
45	17.4279844954585	38.152711977356	20.63668328572414	23.782620241461355
46	22.041837867826537	34.01054931703578	23.525503952367323	20.42210886277036
47	22.625730161413372	25.66473516134084	26.286669415950804	25.422865261294987
48	24.302507305684774	21.273005394853353	31.47730996856283	22.947177330899045
49	20.42959883641927	23.226467494550526	32.284362030392714	24.05957163863749
50	18.971096694967716	34.62585213252576	24.674637617016035	21.72841355549049
51	18.614864074741057	34.94507895409179	22.17867050895407	24.261386462213082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	581.0
1	1114.0
2	1647.0
3	4919.0
4	8191.0
5	6160.5
6	4130.0
7	4488.5
8	4847.0
9	5404.5
10	5962.0
11	6229.0
12	6496.0
13	6274.0
14	6052.0
15	6184.0
16	6316.0
17	6233.5
18	6151.0
19	6119.0
20	6087.0
21	6307.0
22	6527.0
23	7738.5
24	8950.0
25	11819.5
26	18749.0
27	22809.0
28	28591.0
29	34373.0
30	36901.0
31	39429.0
32	47839.5
33	56250.0
34	64464.5
35	72679.0
36	76200.0
37	79721.0
38	115460.5
39	151200.0
40	219122.0
41	287044.0
42	335912.0
43	384780.0
44	372399.0
45	360018.0
46	333660.0
47	307302.0
48	288187.5
49	269073.0
50	252348.5
51	235624.0
52	212447.0
53	189270.0
54	173096.5
55	156923.0
56	148577.5
57	140232.0
58	137167.5
59	134103.0
60	121870.0
61	109637.0
62	97347.0
63	85057.0
64	69933.0
65	54809.0
66	47582.0
67	40355.0
68	34118.5
69	27882.0
70	24427.0
71	20972.0
72	17471.5
73	13971.0
74	11473.0
75	6849.0
76	4723.0
77	3389.5
78	2056.0
79	1645.0
80	1234.0
81	803.5
82	373.0
83	231.5
84	90.0
85	107.5
86	125.0
87	87.5
88	50.0
89	50.5
90	51.0
91	41.5
92	32.0
93	16.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3377849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.967696704913713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.22151403050871	10.64638990367471
2	10.62279182662519	2.9675186878747404
3	3.8365677452475286	1.6076404396041644
4	1.9295549718260325	1.0780575448769738
5	1.0748517913940003	0.750660191246229
6	0.6886394570718952	0.5771224245250008
7	0.45995469387933813	0.44971553634756184
8	0.31437907145247074	0.35129212163364065
9	0.22993375863528034	0.2890480502554587
>10	1.9128162462414322	5.9584073941655005
>50	0.7295342181129513	7.475857804585269
>100	1.9208886296244816	61.82285116826128
>500	0.05232262616621581	4.571740601671575
>1k	0.006250933214547907	1.453698131277897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3921	0.11607978923865454	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3538	0.10474121252903845	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07780099110410205	0.0
2	0.0	0.0	0.0	0.25936624165260197	0.0
3	0.0	0.0	0.0	0.3698507541337697	0.0
4	0.0	0.0	0.0	0.6285360890910162	0.0
5	0.0	0.0	0.0	1.014847022469033	0.0
6	0.0	0.0	0.0	1.418121413953081	0.0
7	0.0	0.0	0.0	1.6284623735400843	0.0
8	0.0	0.0	0.0	2.1593623634449024	0.0
9	0.0	0.0	0.0	2.298119306102789	0.0
10	0.0	0.0	0.0	2.5350156268086583	0.0
11	0.0	0.0	0.0	2.921800234409531	0.0
12	0.0	0.0	0.0	3.2392211730009244	0.0
13	2.960463892850154E-5	0.0	0.0	3.37010328170383	0.0
14	2.960463892850154E-5	0.0	0.0	3.4164937509047917	0.0
15	2.960463892850154E-5	0.0	0.0	3.490357325031403	0.0
16	2.960463892850154E-5	0.0	0.0	3.658570883423149	0.0
17	2.960463892850154E-5	0.0	0.0	3.8616291018337408	0.0
18	2.960463892850154E-5	0.0	0.0	4.090650588584629	0.0
19	5.920927785700308E-5	0.0	0.0	4.247318337794259	0.0
20	8.881391678550463E-5	0.0	0.0	4.390160720624279	0.0
21	8.881391678550463E-5	0.0	0.0	4.596712286428434	0.0
22	8.881391678550463E-5	0.0	0.0	4.804714479540086	0.0
23	8.881391678550463E-5	0.0	0.0	5.038887173464533	0.0
24	8.881391678550463E-5	0.0	0.0	5.222051074515172	0.0
25	8.881391678550463E-5	0.0	0.0	5.356989018751282	0.0
26	8.881391678550463E-5	0.0	0.0	5.490920405263823	0.0
27	8.881391678550463E-5	0.0	0.0	5.6093981702556865	0.0
28	8.881391678550463E-5	0.0	0.0	5.735513932091103	0.0
29	8.881391678550463E-5	0.0	0.0	5.885550242180749	0.0
30	8.881391678550463E-5	0.0	0.0	6.078246836966366	0.0
31	8.881391678550463E-5	0.0	0.0	6.269729641555913	0.0
32	8.881391678550463E-5	0.0	0.0	6.432199899995529	0.0
33	8.881391678550463E-5	0.0	0.0	6.588956463121946	0.0
34	8.881391678550463E-5	0.0	0.0	6.761225857046896	0.0
35	8.881391678550463E-5	0.0	0.0	6.984267206734226	0.0
36	1.1841855571400616E-4	0.0	0.0	7.168852130453433	0.0
37	1.480231946425077E-4	0.0	0.0	7.3586474706240566	0.0
38	1.480231946425077E-4	0.0	0.0	7.532663538245789	0.0
39	1.480231946425077E-4	0.0	0.0	7.738445383437803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	20	7.0352305E-4	45.000004	16
TACCCGC	20	7.0352305E-4	45.000004	23
TCGCACC	70	0.0	45.000004	38
GACACGT	20	7.0352305E-4	45.000004	15
CGCGAAC	20	7.0352305E-4	45.000004	35
CCCGTTG	20	7.0352305E-4	45.000004	30
TACGAAC	20	7.0352305E-4	45.000004	45
TGGTCGA	70	0.0	45.000004	12
GTACCTA	55	1.8189894E-12	45.0	43
CTATCGT	25	3.892418E-5	45.0	13
CTATACG	50	2.1827873E-11	45.0	1
CGGTTCA	50	2.1827873E-11	45.0	41
CACGCGA	50	2.1827873E-11	45.0	37
TATGATC	55	1.8189894E-12	45.0	35
CGTAAGA	25	3.892418E-5	45.0	43
CCGCGTA	55	1.8189894E-12	45.0	36
CACGTAC	25	3.892418E-5	45.0	30
CAATACG	25	3.892418E-5	45.0	1
AATCGTC	25	3.892418E-5	45.0	23
TACGTCA	45	3.8562575E-10	45.0	15
>>END_MODULE