FastQCFastQC Report
Thu 26 May 2016
SRR1545280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545280_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2531775
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG35130.13875640607873924No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30710.12129829862448283No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26760.10569659626151615No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26740.10561760030018466No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.0348964E-445.014
CTCCGAT253.892141E-545.012
ATTACGA351.212702E-745.031
CTACGTC207.0348964E-445.020
CATACCG253.892141E-545.01
CGTAAGA253.892141E-545.037
TCGACCA207.0348964E-445.044
TCGGTAA207.0348964E-445.027
TACCGTT207.0348964E-445.016
GTCGATA302.166542E-644.99999611
GTCGTAC600.044.9999969
TAAACGG2250.043.0000042
GAATAAC900.042.59
TACCGGT900.042.540
AACGCAC556.184564E-1140.90909215
ATAGGGC11700.040.7692344
CGTTGAT8000.040.525
AGTAATG4650.040.1612931
CGTTAGG2900.039.568972
CGGATAT403.4604273E-739.37543
Produced by FastQC (version 0.11.5)