FastQCFastQC Report
Thu 26 May 2016
SRR1545279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545279_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2283265
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36960.1618734575268311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATG302.1664473E-645.00000436
GCCCCGA302.1664473E-645.00000422
GTCATCG302.1664473E-645.00000426
AGACTTC302.1664473E-645.00000413
TCTTGTA453.8562575E-1045.00000418
GTACCCT453.8562575E-1045.00000439
GTACCCC302.1664473E-645.00000413
CGACGGA750.045.00000428
CATGCGT302.1664473E-645.00000414
GCGAATG1500.045.0000041
GCGAAGT302.1664473E-645.0000041
CTATATC302.1664473E-645.00000417
ATTAACG1050.045.0000041
CAATTGT453.8562575E-1045.00000416
TAAGTAT600.045.00000425
CGCGAGA453.8562575E-1045.00000427
ATACTGC453.8562575E-1045.00000441
GTAATCG453.8562575E-1045.00000435
TGCATGC302.1664473E-645.00000443
GTTACAC302.1664473E-645.00000440
Produced by FastQC (version 0.11.5)