##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545279_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2283265
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.397906068721763	31.0	31.0	33.0	30.0	34.0
2	31.697855045296976	31.0	31.0	34.0	30.0	34.0
3	31.775305100371618	31.0	31.0	34.0	30.0	34.0
4	30.292321741015606	35.0	33.0	37.0	0.0	37.0
5	32.897135899687505	35.0	33.0	37.0	19.0	37.0
6	34.531657516757804	35.0	33.0	37.0	30.0	37.0
7	35.546550225225715	37.0	35.0	37.0	33.0	37.0
8	35.74431526782919	37.0	35.0	37.0	35.0	37.0
9	37.833523047040096	39.0	38.0	39.0	35.0	39.0
10	37.341779425515654	39.0	37.0	39.0	34.0	39.0
11	36.79234298252722	39.0	35.0	39.0	33.0	39.0
12	34.951750234861045	35.0	35.0	39.0	31.0	39.0
13	34.20857543911898	35.0	33.0	39.0	26.0	39.0
14	34.84168898485283	35.0	33.0	40.0	27.0	41.0
15	35.39590586287619	35.0	33.0	40.0	30.0	41.0
16	35.729224597232474	35.0	34.0	40.0	32.0	41.0
17	35.68749137747918	35.0	34.0	40.0	31.0	41.0
18	35.7024642343311	36.0	35.0	39.0	31.0	41.0
19	35.64120546673295	36.0	34.0	39.0	31.0	41.0
20	35.461676152352005	35.0	34.0	39.0	31.0	41.0
21	35.26032764484193	35.0	34.0	39.0	31.0	41.0
22	35.12433729768555	35.0	34.0	39.0	30.0	41.0
23	35.146530954576015	35.0	34.0	39.0	30.0	41.0
24	35.06782830727051	35.0	34.0	39.0	30.0	41.0
25	34.98736808911799	35.0	34.0	39.0	30.0	41.0
26	34.805630533468516	35.0	34.0	39.0	30.0	41.0
27	34.77340869325286	35.0	34.0	39.0	29.0	41.0
28	34.895806662827134	35.0	34.0	39.0	30.0	41.0
29	35.03677628308584	35.0	34.0	39.0	30.0	41.0
30	35.03266681703613	35.0	34.0	38.0	30.0	41.0
31	34.7825806465741	35.0	34.0	38.0	30.0	41.0
32	34.586230244846746	35.0	34.0	38.0	29.0	41.0
33	34.41800798418055	35.0	33.0	38.0	29.0	41.0
34	34.37033809041	35.0	34.0	38.0	29.0	41.0
35	34.20470116258954	35.0	33.0	38.0	28.0	41.0
36	33.95510551775637	35.0	33.0	38.0	25.0	41.0
37	33.85904746054444	35.0	33.0	38.0	26.0	41.0
38	33.915743463855485	35.0	33.0	38.0	27.0	41.0
39	33.89658186850848	35.0	33.0	38.0	27.0	41.0
40	33.771605573597455	35.0	33.0	38.0	25.0	41.0
41	33.83237863322917	35.0	33.0	38.0	26.0	41.0
42	33.783045332013586	35.0	33.0	38.0	26.0	40.0
43	33.64881956321321	35.0	33.0	38.0	26.0	40.0
44	33.60067885243281	35.0	33.0	37.0	26.0	40.0
45	33.52810339579506	35.0	33.0	37.0	26.0	40.0
46	33.50943013623036	35.0	33.0	37.0	26.0	40.0
47	33.43367414645256	35.0	33.0	37.0	26.0	40.0
48	33.42036206922981	35.0	33.0	37.0	26.0	40.0
49	33.450015657402886	35.0	33.0	37.0	25.0	40.0
50	33.199270343127054	35.0	33.0	36.0	24.0	40.0
51	33.10920370609632	35.0	32.0	36.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	21.0
15	46.0
16	116.0
17	377.0
18	959.0
19	1879.0
20	3629.0
21	6350.0
22	10149.0
23	15113.0
24	20737.0
25	27774.0
26	35296.0
27	42756.0
28	51446.0
29	62452.0
30	76910.0
31	99540.0
32	130679.0
33	205328.0
34	331836.0
35	484218.0
36	123611.0
37	142541.0
38	184833.0
39	224470.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.192787521378378	24.786260026759923	31.401961664546164	12.618990787315532
2	26.331415757697858	27.440091272804512	32.944839955064346	13.283653014433277
3	27.00479357411426	26.761238839994483	31.821404874160468	14.41256271173079
4	20.66037012786514	39.742079872463336	27.17945573553661	12.418094264134911
5	24.72034564538063	32.42151042476454	29.9964743470425	12.861669582812333
6	20.945619540438802	40.238868462486835	27.703617407528252	11.111894589546111
7	88.74747346453434	4.705936455032596	4.897679419603068	1.6489106608299955
8	91.38746488033583	3.116852402152181	3.409284511434284	2.0863982060777
9	89.95355335451644	2.828843782916131	4.589392821245015	2.6282100413224043
10	72.65573641254957	12.662437343015377	7.150505964047099	7.531320280387953
11	68.03428423770347	12.33794588013218	11.598828870061075	8.028941012103282
12	57.14544741849939	20.675217287524664	13.281112792426637	8.898222501549316
13	18.075738033036025	57.94679986773327	14.529281533242965	9.448180565987743
14	10.153179766693748	56.718339745933996	23.31963219337221	9.80884829400004
15	8.64227323591436	19.29876733537281	62.425474047033525	9.633485381679305
16	10.169253240425444	12.779287555320998	58.33694293040887	18.71451627384469
17	11.960460130558651	14.788646959507549	24.232973395554176	49.01791951437963
18	21.81551418692092	21.440831440940933	36.40295804472981	20.340696327408338
19	35.87827080956437	22.585858408901288	20.127098694194498	21.408772087339837
20	38.19587301517783	22.913109078446873	19.837863760886272	19.053154145489025
21	20.600674910709007	32.315609445246174	24.286274260762546	22.797441383282273
22	22.06769691647706	25.38224866583598	21.387092606421067	31.162961811265884
23	18.485721105522135	35.31018081563025	20.21180195903673	25.992296119810888
24	20.175713287769923	21.345047552517997	43.861969591790704	14.617269567921376
25	13.146393432212205	24.367473771112856	41.21956934477601	21.266563451898925
26	12.945978675274223	43.410861200955644	23.723965461740097	19.919194662030034
27	15.518522817106206	39.69823914438315	28.017860388522575	16.765377649988064
28	10.000985430950854	28.99321804521157	47.01780126266553	13.98799526117205
29	10.687546123643116	24.404482177933794	44.23529463290507	20.67267706551802
30	17.606716697361016	34.22414831392764	30.569250612609572	17.599884376101766
31	36.915206951448916	25.278712720599668	19.21835616978318	18.587724158168236
32	39.418201566616226	25.800640749102712	21.38166178695859	13.399495897322474
33	32.92613866546371	27.720479226020633	20.73924840086455	18.614133707651103
34	16.831598609885408	27.599205523668957	27.068649499729553	28.500546366716083
35	19.08963698913617	24.230608361272125	34.37419660004423	22.305558049547468
36	38.49264102064368	20.37231771169794	24.688198697917237	16.446842569741136
37	19.3765506850935	34.56344313953921	29.495262266972954	16.564743908394338
38	19.122440890566796	37.742311996198424	20.019358243567872	23.115888869666904
39	20.95477309904895	34.73503951578113	25.746025975959864	18.564161409210055
40	27.216026173046053	24.391781067900574	21.048586125570182	27.34360663348319
41	14.997383133363845	20.66032633093399	25.53203417036568	38.810256365336485
42	23.04016397571022	23.870159617915572	20.519081227978354	32.57059517839585
43	22.53825114474229	25.259836243274435	23.581625435505735	28.620287176477543
44	19.044219571534622	32.19162033316326	28.1324769573396	20.631683137962522
45	14.613152656393366	44.0886625074181	17.836256413513105	23.461928422675424
46	23.425357985165977	35.86228492969498	23.052295725638505	17.66006135950054
47	21.464262799105665	25.441199335162583	26.230726612986228	26.863811252745517
48	24.436453937672585	19.087928908821358	34.10318119009401	22.372435963412045
49	20.251438181726606	18.7753502112107	36.22579945823196	24.74741214883073
50	18.498028043175015	37.23474936111227	23.439854769376307	20.82736782633641
51	15.884095801407195	39.49607251019921	20.27298627185193	24.34684541654166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	885.0
1	999.5
2	1114.0
3	2748.5
4	4383.0
5	3400.5
6	2418.0
7	3025.5
8	3633.0
9	4321.0
10	5009.0
11	5353.0
12	5697.0
13	5724.5
14	5752.0
15	5650.0
16	5548.0
17	5367.0
18	5186.0
19	5010.5
20	4835.0
21	4903.5
22	4972.0
23	6147.0
24	7322.0
25	7486.0
26	7744.0
27	7838.0
28	9067.0
29	10296.0
30	13892.5
31	17489.0
32	20618.5
33	23748.0
34	26913.0
35	30078.0
36	35428.0
37	40778.0
38	66652.5
39	92527.0
40	165554.0
41	238581.0
42	287073.5
43	335566.0
44	320592.0
45	305618.0
46	271475.5
47	237333.0
48	212127.5
49	186922.0
50	177132.0
51	167342.0
52	149252.5
53	131163.0
54	113744.5
55	96326.0
56	87434.5
57	78543.0
58	69358.5
59	60174.0
60	55570.0
61	50966.0
62	45153.0
63	39340.0
64	32897.5
65	26455.0
66	21336.5
67	16218.0
68	12566.0
69	8914.0
70	7837.5
71	6761.0
72	5697.5
73	4634.0
74	3361.0
75	1978.5
76	1869.0
77	1253.0
78	637.0
79	548.5
80	460.0
81	281.5
82	103.0
83	85.0
84	67.0
85	43.0
86	19.0
87	11.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2283265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.899754957826794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.20693947224704	10.870572946658257
2	10.241679636408364	2.8471367460528176
3	3.585124055758994	1.4949703760538053
4	1.6584840356388404	0.9221008678739024
5	0.9370545043878755	0.6512413996559651
6	0.5047991399021585	0.42099506085370375
7	0.3495651815166263	0.3401209255408545
8	0.25445695331509116	0.28295114387159515
9	0.1759871519932965	0.22015604595893765
>10	1.468242638408253	4.553397516602012
>50	0.590998050019768	6.078234349415785
>100	1.95019109812439	63.38291376688756
>500	0.0662354819741126	5.8700423948862035
>1k	0.010242600305275143	2.0651664596886072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3696	0.1618734575268311	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1001197846066926	0.0
2	0.0	0.0	0.0	0.3623758083271105	0.0
3	0.0	0.0	0.0	0.5007741107580592	0.0
4	0.0	0.0	0.0	0.8360834156350665	0.0
5	0.0	0.0	0.0	1.3327406148651164	0.0
6	0.0	0.0	0.0	1.8388141542922087	0.0
7	0.0	0.0	0.0	2.0828506546546284	0.0
8	0.0	0.0	0.0	2.5962820785147587	0.0
9	0.0	0.0	0.0	2.745016456696879	0.0
10	0.0	0.0	0.0	3.0735810341769354	0.0
11	4.379693114903439E-5	0.0	0.0	3.4411248803796317	0.0
12	4.379693114903439E-5	0.0	0.0	3.8433120991212144	0.0
13	1.3139079344710316E-4	0.0	0.0	3.971374325800991	0.0
14	1.3139079344710316E-4	0.0	0.0	4.034967469829389	0.0
15	1.3139079344710316E-4	0.0	0.0	4.127554182278447	0.0
16	1.3139079344710316E-4	0.0	0.0	4.319384740711218	0.0
17	1.3139079344710316E-4	0.0	0.0	4.516208149294979	0.0
18	1.3139079344710316E-4	0.0	0.0	4.758317584686841	0.0
19	1.3139079344710316E-4	0.0	0.0	4.904117568481976	0.0
20	1.3139079344710316E-4	0.0	0.0	5.029639573155109	0.0
21	1.3139079344710316E-4	0.0	0.0	5.205659439443078	0.0
22	1.7518772459613756E-4	0.0	0.0	5.364817487238669	0.0
23	1.7518772459613756E-4	0.0	0.0	5.5586189075731465	0.0
24	1.7518772459613756E-4	0.0	0.0	5.680637157754356	0.0
25	2.1898465574517192E-4	0.0	0.0	5.774231199619843	0.0
26	2.627815868942063E-4	0.0	0.0	5.87960661596442	0.0
27	2.627815868942063E-4	0.0	0.0	5.967069087469041	0.0
28	2.627815868942063E-4	0.0	0.0	6.07686799385967	0.0
29	2.627815868942063E-4	0.0	0.0	6.1845646475551455	0.0
30	2.627815868942063E-4	0.0	0.0	6.3453869787344	0.0
31	2.627815868942063E-4	0.0	0.0	6.492719855119752	0.0
32	2.627815868942063E-4	0.0	0.0	6.605759734415409	0.0
33	2.627815868942063E-4	0.0	0.0	6.717135330327404	0.0
34	2.627815868942063E-4	0.0	0.0	6.8490078900171465	0.0
35	2.627815868942063E-4	0.0	0.0	7.044035624423796	0.0
36	2.627815868942063E-4	0.0	0.0	7.178711187707077	0.0
37	2.627815868942063E-4	0.0	0.0	7.317459865587218	0.0
38	2.627815868942063E-4	0.0	0.0	7.4386459740765964	0.0
39	2.627815868942063E-4	0.0	0.0	7.5689856411761225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATG	30	2.1664473E-6	45.000004	36
GCCCCGA	30	2.1664473E-6	45.000004	22
GTCATCG	30	2.1664473E-6	45.000004	26
AGACTTC	30	2.1664473E-6	45.000004	13
TCTTGTA	45	3.8562575E-10	45.000004	18
GTACCCT	45	3.8562575E-10	45.000004	39
GTACCCC	30	2.1664473E-6	45.000004	13
CGACGGA	75	0.0	45.000004	28
CATGCGT	30	2.1664473E-6	45.000004	14
GCGAATG	150	0.0	45.000004	1
GCGAAGT	30	2.1664473E-6	45.000004	1
CTATATC	30	2.1664473E-6	45.000004	17
ATTAACG	105	0.0	45.000004	1
CAATTGT	45	3.8562575E-10	45.000004	16
TAAGTAT	60	0.0	45.000004	25
CGCGAGA	45	3.8562575E-10	45.000004	27
ATACTGC	45	3.8562575E-10	45.000004	41
GTAATCG	45	3.8562575E-10	45.000004	35
TGCATGC	30	2.1664473E-6	45.000004	43
GTTACAC	30	2.1664473E-6	45.000004	40
>>END_MODULE