FastQCFastQC Report
Thu 26 May 2016
SRR1545278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545278_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2711717
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT36400.13423229636425926No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.12969642481129115No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28500.1050994628126755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACA351.2127384E-745.00000411
ACGTTCA351.2127384E-745.00000419
GCAACCG351.2127384E-745.0000041
GACGTAT351.2127384E-745.00000417
TCCACGT351.2127384E-745.00000416
ATCTACG207.034985E-445.023
ACGTTCC207.034985E-445.015
CGCATTA406.8212103E-945.036
ATTACCG406.8212103E-945.01
CCAATCG253.892213E-545.029
TCGTAGT207.034985E-445.034
TATAGCG1650.045.01
CGGTCGA207.034985E-445.035
CGCCGTG253.892213E-545.024
ACCTTCG207.034985E-445.01
TCGCGAC253.892213E-545.022
ATCGATA253.892213E-545.036
GATAGTC207.034985E-445.09
CGGACTA207.034985E-445.036
CGTGAAC302.1665983E-644.99999644