##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545278_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2711717
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27320328780621	31.0	31.0	33.0	30.0	34.0
2	31.58897923345246	31.0	31.0	34.0	30.0	34.0
3	31.67844321512901	31.0	31.0	34.0	30.0	34.0
4	30.265370243281286	35.0	33.0	37.0	0.0	37.0
5	32.828901762241415	35.0	33.0	37.0	19.0	37.0
6	34.43388709072517	35.0	33.0	37.0	30.0	37.0
7	35.48308617750303	36.0	35.0	37.0	33.0	37.0
8	35.66808888980672	37.0	35.0	37.0	35.0	37.0
9	37.6948346748573	39.0	37.0	39.0	35.0	39.0
10	37.150038518031195	39.0	37.0	39.0	33.0	39.0
11	36.663301517083084	39.0	35.0	39.0	32.0	39.0
12	35.362927252364464	35.0	35.0	39.0	31.0	39.0
13	34.80661661965463	35.0	34.0	39.0	29.0	39.0
14	35.582256186762855	36.0	34.0	40.0	29.0	41.0
15	35.97053342955773	36.0	34.0	40.0	30.0	41.0
16	36.21972056818613	36.0	35.0	40.0	31.0	41.0
17	36.158539773877585	36.0	35.0	40.0	31.0	41.0
18	36.15531229844412	36.0	35.0	40.0	31.0	41.0
19	36.117451046698456	36.0	35.0	40.0	31.0	41.0
20	35.97527101832529	36.0	34.0	40.0	31.0	41.0
21	35.80558553860893	36.0	34.0	40.0	30.0	41.0
22	35.66037790816667	35.0	34.0	40.0	30.0	41.0
23	35.62922495230882	35.0	34.0	40.0	30.0	41.0
24	35.54538950782843	35.0	34.0	40.0	30.0	41.0
25	35.43423078440708	35.0	34.0	40.0	30.0	41.0
26	35.24440234729509	35.0	34.0	40.0	29.0	41.0
27	35.22929531363339	35.0	34.0	40.0	29.0	41.0
28	35.286033903980396	36.0	34.0	40.0	29.0	41.0
29	35.379003413704304	36.0	34.0	40.0	30.0	41.0
30	35.36033332386823	36.0	34.0	40.0	30.0	41.0
31	35.22888487257335	35.0	34.0	40.0	29.0	41.0
32	35.08828059860229	35.0	34.0	40.0	29.0	41.0
33	34.93158504371953	35.0	34.0	40.0	29.0	41.0
34	34.84636228633003	35.0	34.0	40.0	28.0	41.0
35	34.723785704776716	35.0	34.0	40.0	27.0	41.0
36	34.5192363362401	35.0	33.0	40.0	26.0	41.0
37	34.45206450378118	35.0	33.0	40.0	26.0	41.0
38	34.443004561316684	35.0	33.0	40.0	27.0	41.0
39	34.403823850350165	35.0	33.0	40.0	26.0	41.0
40	34.2516848181429	35.0	33.0	39.0	25.0	41.0
41	34.28535462955758	35.0	33.0	39.0	26.0	41.0
42	34.198552430065526	35.0	33.0	39.0	26.0	41.0
43	34.110792534766716	35.0	33.0	39.0	26.0	41.0
44	34.042648624469294	35.0	33.0	39.0	26.0	41.0
45	33.94707227929758	35.0	33.0	39.0	26.0	41.0
46	33.857190849930134	35.0	33.0	39.0	25.0	41.0
47	33.79923679351496	35.0	33.0	39.0	25.0	41.0
48	33.75353844077387	35.0	33.0	38.0	25.0	41.0
49	33.723374157406546	35.0	33.0	38.0	24.0	40.0
50	33.55989655262699	35.0	33.0	38.0	24.0	40.0
51	33.46535645128161	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	9.0
15	67.0
16	227.0
17	550.0
18	1358.0
19	2770.0
20	4702.0
21	7851.0
22	12328.0
23	18169.0
24	24974.0
25	33048.0
26	42133.0
27	50957.0
28	60124.0
29	73234.0
30	89296.0
31	111870.0
32	142783.0
33	211405.0
34	322411.0
35	448135.0
36	183472.0
37	222492.0
38	288595.0
39	358495.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.73391618668172	24.636199131399035	30.543969005615264	13.08591567630398
2	27.53432603770969	28.41284691581017	29.873102539829933	14.179724506650215
3	28.50278992977512	26.34710775497591	31.007107305076453	14.142995010172521
4	21.717421102570807	39.061893258035404	26.531455900449792	12.689229738943997
5	24.326727309671327	32.26435501934752	29.22030580624748	14.188611864733671
6	22.636469808612034	40.32327119680999	25.99371542089385	11.046543573684128
7	88.94858866172244	4.173776245825063	5.047945637395053	1.8296894550574414
8	90.92810938604582	3.1309683126963472	3.805669986949228	2.1352523143086097
9	87.66530578227743	4.421405331013524	4.933036891386528	2.980251995322521
10	61.85973683832052	20.293489327979284	8.751134428850799	9.0956394048494
11	55.38778567232495	17.81989049742285	15.796338629731643	10.995985200520556
12	50.1334394407676	20.303556750206603	16.91765770543165	12.645346103594143
13	21.313544149334167	45.42218085441807	18.16712437175413	15.097150624493633
14	13.680741758819229	47.192719594264446	23.100861926226077	16.02567672069025
15	12.946446845301335	23.350666754679782	50.27095379053198	13.431932609486905
16	13.833043787386368	16.67095054535558	49.415517917245786	20.08048775001226
17	14.92976590108776	19.313962334565147	25.51811269391312	40.23815907043397
18	22.029289929590735	24.401181981748095	31.531682694027435	22.03784539463373
19	32.68980502021413	25.44085536949468	20.11400894709883	21.75533066319236
20	36.53640848215356	22.94454030416891	21.18414274055884	19.33490847311869
21	22.894940733122223	30.315294700737578	24.129250950596983	22.660513615543216
22	24.03119499564298	25.526926297987586	22.26637219149343	28.175506514875998
23	21.800246854668092	32.1758502085579	20.894178854209343	25.129724082564664
24	22.10739542511258	22.31032958085228	37.979110652033384	17.603164342001765
25	17.820517406499278	25.501296779863093	34.29896998838743	22.379215825250203
26	16.491248902448152	38.97453163438515	23.705091644887723	20.829127818278973
27	19.2444491810908	36.17328061888464	26.452907880874	18.12936231915056
28	15.94687056208299	29.180036117338204	39.24484007733845	15.628253243240353
29	15.82952793377775	24.629893163630275	37.91125696376134	21.629321938830635
30	19.370273520430047	32.30340776710844	28.506809523265147	19.819509189196367
31	31.403424472391478	28.281085378747118	19.949389999030135	20.366100149831272
32	34.07903553357522	27.349535368181854	22.063917436812176	16.507511661430748
33	29.228492501245523	28.91879941749084	21.75311804292262	20.099590038341024
34	19.10789363344331	27.77233022472478	27.107032186618298	26.01274395521361
35	21.54225533121635	24.879661115079486	29.42512068921646	24.152962864487705
36	31.76334403626927	25.38815813007036	23.39727928836232	19.45121854529805
37	21.738772888173802	32.918848095136774	26.851880192512713	18.49049882417671
38	20.506749044977777	33.59384478542562	22.1046296497754	23.794776519821205
39	21.419565537259235	31.998471816933698	25.615578616795187	20.966384029011877
40	25.544110982082568	25.130314114636594	23.592727412189397	25.732847491091437
41	16.836565172545658	22.932223384667353	26.8827093682711	33.34850207451589
42	23.594460631400697	24.74435938558485	21.838267046303137	29.822912936711315
43	22.905635064425972	27.50799585649977	23.325922284663186	26.26044679441107
44	20.65433081696947	31.199900284579847	26.11902348217015	22.026745416280534
45	18.006451263166472	38.328299007602936	19.98099359188293	23.684256137347663
46	22.09832368200664	34.08279698803378	22.64469338061457	21.17418594934501
47	21.998386999823357	26.601005930928633	26.192777491161507	25.207829578086503
48	24.459447648851263	20.95321893840692	31.38328962793684	23.204043784804977
49	19.966095282066675	23.053769991485098	32.016725934159055	24.963408792289165
50	18.344355255360348	33.595061726573974	25.508340287721765	22.55224273034391
51	17.643065260866088	35.11332487866544	21.962653182467047	25.280956678001427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	657.0
1	1115.0
2	1573.0
3	4274.0
4	6975.0
5	5299.0
6	3623.0
7	4028.0
8	4433.0
9	5045.5
10	5658.0
11	5867.0
12	6076.0
13	5901.0
14	5726.0
15	5521.0
16	5316.0
17	5316.5
18	5317.0
19	5127.0
20	4937.0
21	4777.0
22	4617.0
23	5386.5
24	6156.0
25	8581.0
26	13152.5
27	15299.0
28	19063.5
29	22828.0
30	28085.5
31	33343.0
32	39000.0
33	44657.0
34	53509.5
35	62362.0
36	62928.0
37	63494.0
38	83398.5
39	103303.0
40	156705.5
41	210108.0
42	258363.0
43	306618.0
44	298396.0
45	290174.0
46	277469.5
47	264765.0
48	245071.5
49	225378.0
50	209500.5
51	193623.0
52	171066.0
53	148509.0
54	134773.0
55	121037.0
56	114874.5
57	108712.0
58	109848.0
59	110984.0
60	103539.5
61	96095.0
62	83350.5
63	70606.0
64	61891.5
65	53177.0
66	43310.5
67	33444.0
68	28492.5
69	23541.0
70	19540.0
71	15539.0
72	12635.0
73	9731.0
74	8070.5
75	4553.0
76	2696.0
77	2352.5
78	2009.0
79	1365.5
80	722.0
81	469.0
82	216.0
83	161.5
84	107.0
85	113.5
86	120.0
87	71.5
88	23.0
89	16.5
90	10.0
91	6.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2711717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.584199343979341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21661045085244	12.189432492764622
2	9.772162956511897	3.0458267107226384
3	3.4596314868849216	1.6174676024496681
4	1.6799418879343366	1.047221970714788
5	0.9551572740059759	0.7442680681480512
6	0.5830308759664289	0.5451641636853433
7	0.4159913399357303	0.4538024376849247
8	0.2744558067939926	0.3421739203352208
9	0.1997047404037622	0.28010146359508875
>10	1.7590248163182924	6.350353385440911
>50	0.770212335235196	8.851653753668616
>100	1.8806446327082555	60.420709670883674
>500	0.02931676303978947	2.923620217306156
>1k	0.004114633409093259	1.188204142600314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3640	0.13423229636425926	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3517	0.12969642481129115	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2850	0.1050994628126755	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08478023333555824	0.0
2	0.0	0.0	0.0	0.303534624003906	0.0
3	0.0	0.0	0.0	0.4331204178017101	0.0
4	0.0	0.0	0.0	0.6814870430800855	0.0
5	0.0	0.0	0.0	1.1279938135137257	0.0
6	0.0	0.0	0.0	1.5323132908043133	0.0
7	0.0	0.0	0.0	1.7498507403243038	0.0
8	0.0	0.0	0.0	2.2182624514283753	0.0
9	0.0	0.0	0.0	2.3536379349320007	0.0
10	3.687700449567562E-5	0.0	0.0	2.6626672326057625	0.0
11	3.687700449567562E-5	0.0	0.0	3.029224657292778	0.0
12	3.687700449567562E-5	0.0	0.0	3.3889598361480937	0.0
13	3.687700449567562E-5	0.0	0.0	3.518766891972872	0.0
14	3.687700449567562E-5	0.0	0.0	3.578544516260362	0.0
15	3.687700449567562E-5	0.0	0.0	3.6770429952683115	0.0
16	3.687700449567562E-5	0.0	0.0	3.874593108351646	0.0
17	3.687700449567562E-5	0.0	0.0	4.080403670442012	0.0
18	3.687700449567562E-5	0.0	0.0	4.320436092704364	0.0
19	7.375400899135124E-5	0.0	0.0	4.476536452734559	0.0
20	7.375400899135124E-5	0.0	0.0	4.6132026313955325	0.0
21	7.375400899135124E-5	0.0	0.0	4.813002241753103	0.0
22	7.375400899135124E-5	0.0	0.0	5.010773616863411	0.0
23	7.375400899135124E-5	0.0	0.0	5.22849545140588	0.0
24	7.375400899135124E-5	0.0	0.0	5.39060676316887	0.0
25	7.375400899135124E-5	0.0	0.0	5.525576599623043	0.0
26	7.375400899135124E-5	0.0	0.0	5.658444446820963	0.0
27	7.375400899135124E-5	0.0	0.0	5.778552850463378	0.0
28	1.1063101348702685E-4	0.0	0.0	5.902459585568848	0.0
29	1.1063101348702685E-4	0.0	0.0	6.035548694793741	0.0
30	1.1063101348702685E-4	0.0	0.0	6.211120113197653	0.0
31	1.1063101348702685E-4	0.0	0.0	6.381454996963178	0.0
32	1.1063101348702685E-4	0.0	0.0	6.5357852607775815	0.0
33	1.1063101348702685E-4	0.0	0.0	6.680195610382647	0.0
34	1.1063101348702685E-4	0.0	0.0	6.848059734846962	0.0
35	1.1063101348702685E-4	0.0	0.0	7.067514788600728	0.0
36	1.1063101348702685E-4	0.0	0.0	7.245704474323833	0.0
37	1.1063101348702685E-4	0.0	0.0	7.415412449012932	0.0
38	1.1063101348702685E-4	0.0	0.0	7.581949001315403	0.0
39	1.4750801798270248E-4	0.0	0.0	7.745756655285194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACA	35	1.2127384E-7	45.000004	11
ACGTTCA	35	1.2127384E-7	45.000004	19
GCAACCG	35	1.2127384E-7	45.000004	1
GACGTAT	35	1.2127384E-7	45.000004	17
TCCACGT	35	1.2127384E-7	45.000004	16
ATCTACG	20	7.034985E-4	45.0	23
ACGTTCC	20	7.034985E-4	45.0	15
CGCATTA	40	6.8212103E-9	45.0	36
ATTACCG	40	6.8212103E-9	45.0	1
CCAATCG	25	3.892213E-5	45.0	29
TCGTAGT	20	7.034985E-4	45.0	34
TATAGCG	165	0.0	45.0	1
CGGTCGA	20	7.034985E-4	45.0	35
CGCCGTG	25	3.892213E-5	45.0	24
ACCTTCG	20	7.034985E-4	45.0	1
TCGCGAC	25	3.892213E-5	45.0	22
ATCGATA	25	3.892213E-5	45.0	36
GATAGTC	20	7.034985E-4	45.0	9
CGGACTA	20	7.034985E-4	45.0	36
CGTGAAC	30	2.1665983E-6	44.999996	44
>>END_MODULE