FastQCFastQC Report
Thu 26 May 2016
SRR1545277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545277_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1277149
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.16920500270524427No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16960.13279578185474053No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15390.12050277610521559No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14910.11674440492064747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAA453.8562575E-1045.00000421
AACCGTA207.033582E-445.017
CTATCGA207.033582E-445.014
CTATACG502.1827873E-1145.01
GTTAGTC207.033582E-445.017
CCGTCCA207.033582E-445.037
TACGCCG207.033582E-445.013
CGCTATA351.2120654E-745.039
TTGCGTA207.033582E-445.037
ACGCTCA207.033582E-445.026
CTATTCG253.8910504E-545.030
ACCGTTA207.033582E-445.014
CAGTACG253.8910504E-545.01
CTCTACG253.8910504E-545.01
TCGAACG351.2120654E-745.01
TACACCG253.8910504E-545.016
TCTAGCG302.1656924E-644.9999961
ATAGCGG2350.043.0851062
ATAGGGC6700.042.9850774
CGTATTT3000.042.0000041