##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545276_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1978146
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297676207923985	31.0	31.0	33.0	30.0	34.0
2	31.596322010609935	31.0	31.0	34.0	30.0	34.0
3	31.686583295671806	31.0	31.0	34.0	30.0	34.0
4	30.252381270138805	35.0	33.0	37.0	0.0	37.0
5	32.81788351314817	35.0	33.0	37.0	19.0	37.0
6	34.435567951000586	35.0	33.0	37.0	30.0	37.0
7	35.49421529047906	36.0	35.0	37.0	33.0	37.0
8	35.69613618003929	37.0	35.0	37.0	35.0	37.0
9	37.74880418331104	39.0	37.0	39.0	35.0	39.0
10	37.20954317830939	39.0	37.0	39.0	34.0	39.0
11	36.706891705667836	39.0	35.0	39.0	32.0	39.0
12	35.074493490369264	35.0	35.0	39.0	31.0	39.0
13	34.42113372824857	35.0	33.0	39.0	27.0	39.0
14	35.074978793274106	35.0	33.0	40.0	27.0	41.0
15	35.600037105451264	35.0	34.0	40.0	30.0	41.0
16	35.90226606125129	35.0	34.0	40.0	31.0	41.0
17	35.874561331671174	35.0	34.0	40.0	31.0	41.0
18	35.876984813052225	36.0	35.0	40.0	31.0	41.0
19	35.831460367434964	36.0	35.0	40.0	31.0	41.0
20	35.686794099121094	35.0	34.0	40.0	31.0	41.0
21	35.4634066444034	35.0	34.0	40.0	30.0	41.0
22	35.31737596719352	35.0	34.0	40.0	30.0	41.0
23	35.34930990937979	35.0	34.0	40.0	30.0	41.0
24	35.262043347659876	35.0	34.0	40.0	30.0	41.0
25	35.13439250692315	35.0	34.0	39.0	30.0	41.0
26	34.94630932196107	35.0	34.0	39.0	29.0	41.0
27	34.90869834683588	35.0	34.0	39.0	29.0	41.0
28	34.99356013155753	35.0	34.0	39.0	29.0	41.0
29	35.108590063625236	35.0	34.0	39.0	30.0	41.0
30	35.09624466545947	36.0	34.0	39.0	30.0	41.0
31	34.88492558183268	35.0	34.0	39.0	29.0	41.0
32	34.759628965708295	35.0	34.0	39.0	29.0	41.0
33	34.63875719992357	35.0	34.0	39.0	29.0	41.0
34	34.60173718218979	35.0	34.0	39.0	29.0	41.0
35	34.46186125796579	35.0	33.0	39.0	27.0	41.0
36	34.220795128367676	35.0	33.0	39.0	26.0	41.0
37	34.111135881780214	35.0	33.0	39.0	26.0	41.0
38	34.15084933063586	35.0	33.0	39.0	27.0	41.0
39	34.12697748295626	35.0	33.0	39.0	26.0	41.0
40	34.00166519559224	35.0	33.0	39.0	25.0	41.0
41	34.065090241064105	35.0	33.0	39.0	26.0	41.0
42	34.019460646484134	35.0	33.0	38.0	26.0	41.0
43	33.90224280715377	35.0	33.0	38.0	26.0	41.0
44	33.84011695800007	35.0	33.0	38.0	26.0	41.0
45	33.7546616882677	35.0	33.0	38.0	26.0	41.0
46	33.71743844994252	35.0	33.0	38.0	26.0	40.0
47	33.659457390910475	35.0	33.0	38.0	26.0	40.0
48	33.627525976343506	35.0	33.0	38.0	26.0	40.0
49	33.6407545246913	35.0	33.0	38.0	25.0	40.0
50	33.411126883455516	35.0	33.0	38.0	24.0	40.0
51	33.32015280975216	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	1.0
14	13.0
15	39.0
16	166.0
17	417.0
18	1021.0
19	1960.0
20	3558.0
21	5783.0
22	8974.0
23	12733.0
24	17920.0
25	24043.0
26	30938.0
27	37623.0
28	45245.0
29	55090.0
30	67412.0
31	84255.0
32	109734.0
33	166097.0
34	262403.0
35	377331.0
36	119062.0
37	138949.0
38	180890.0
39	226330.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.590489276322376	24.525894448640294	31.302138467029227	12.581477808008104
2	26.54809099025047	27.308550531659442	32.217136652198576	13.926221825891517
3	29.3097172807265	26.16419617156671	30.774573767558106	13.751512780148683
4	21.733785069453923	39.50871169266576	25.84607000696612	12.911433230914199
5	23.430272588575363	32.231291320256446	30.523631723846474	13.814804367321726
6	21.125589314438873	40.83566127070499	26.533279141175626	11.505470273680507
7	89.18805790876912	4.816884092478513	4.332541682969811	1.662516315782556
8	92.0870350317924	2.3328409530944634	3.514401869225022	2.0657221458881194
9	89.5826192808822	3.241520090023689	4.880832860668525	2.2950277684255864
10	67.54511547681516	16.669244838348636	7.236574044585183	8.549065640251023
11	62.52571852633729	14.495947215220717	13.457196789316866	9.521137469125131
12	53.35121876747216	20.988086824733866	15.414635724562292	10.246058683231672
13	18.421339981983127	54.20712121349991	16.340907091792012	11.030631712724945
14	10.835752264999652	53.33822680429048	24.3575550035235	11.468465927186365
15	9.86079895012805	21.3200643430768	58.482993671852334	10.33614303494282
16	10.975074640597812	13.837199074284708	55.05822118286516	20.12950510225231
17	13.005915640200472	16.256181292988487	25.058514386703507	45.679388680107536
18	21.73322899320879	22.41518067928252	34.44366593770126	21.407924389807427
19	33.89699243635202	23.0852020022789	21.276842053114382	21.740963508254698
20	36.99302275969519	22.452437787706266	20.740329581335253	19.814209871263294
21	21.531727182927852	30.477072976413268	25.33018290864274	22.66101693201614
22	22.54171330124268	26.11794073844903	20.956946555006557	30.38339940530173
23	18.96265493042475	34.512265525396	20.495605481091893	26.029474063087356
24	19.49977403083493	22.460323959909935	42.82600980918496	15.213892200070166
25	14.564546802915457	24.65859446168281	38.82413128252414	21.952727452877593
26	14.064634258543101	42.24698278084631	23.454133314730054	20.234249645880535
27	16.032941956761533	40.290099921846014	26.820214483662987	16.856743637729473
28	12.351919423541032	29.689416251378812	43.5861154838925	14.372548841187657
29	11.457243297511912	24.28991591116126	42.12257335909483	22.130267432232
30	17.948725726008092	33.53422851498322	29.373918810846117	19.14312694816257
31	34.00264692292682	27.62748553443477	20.127584111587314	18.242283431051096
32	36.331190923218	26.29932269913343	21.536276897660738	15.833209479987827
33	31.23940295610132	27.98135223588148	20.85275808762346	19.926486720393743
34	17.819614932365962	27.690979331151492	26.68286365111574	27.806542085366804
35	20.077941668612933	23.415915710973813	32.02898067180077	24.477161948612487
36	35.59221614582543	21.99428151410462	24.47877962496196	17.934722715107984
37	19.412975584208645	34.80127351570612	28.41342347834791	17.372327421737324
38	19.988059526445472	36.587693729380945	20.50940628244831	22.914840461725273
39	19.737774663750805	34.69521461004395	25.2278143271528	20.339196399052444
40	25.473701132272343	25.04264093752433	21.786612312741322	27.697045617462006
41	15.77856235080727	21.240697097180895	26.40907192896783	36.571668623044005
42	22.379490694822323	24.555113727702608	20.993900349114778	32.07149522836029
43	21.8232122401481	27.374116976198927	23.722920350671792	27.079750432981186
44	18.978578931989855	33.3368720003478	26.664513135026436	21.020035932635913
45	15.687011979904417	42.33625829438272	18.677387816672784	23.29934190904008
46	22.2115556687929	34.874675580063354	23.137675378864856	19.77609337227889
47	21.040661306091664	26.600210500134974	26.26059957151798	26.098528622255383
48	24.09958617816885	19.69379408799957	32.91496178745148	23.291657946380095
49	20.615111321409035	21.168963261559057	33.930609772989456	24.285315644042452
50	18.398237541617252	35.94689168544688	24.33273378203631	21.32213699089956
51	16.14157903410567	38.40252438394335	20.749580667958785	24.706315913992192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	566.5
2	753.0
3	2262.0
4	3771.0
5	2901.0
6	2031.0
7	2451.5
8	2872.0
9	3330.0
10	3788.0
11	4066.5
12	4345.0
13	4288.5
14	4232.0
15	4085.0
16	3938.0
17	3722.0
18	3506.0
19	3502.0
20	3498.0
21	4182.5
22	4867.0
23	4987.5
24	5108.0
25	5953.5
26	8994.5
27	11190.0
28	13325.0
29	15460.0
30	17948.0
31	20436.0
32	25024.5
33	29613.0
34	32897.5
35	36182.0
36	39396.0
37	42610.0
38	64469.0
39	86328.0
40	132980.5
41	179633.0
42	225151.5
43	270670.0
44	263837.0
45	257004.0
46	226128.5
47	195253.0
48	180811.5
49	166370.0
50	151205.0
51	136040.0
52	121898.0
53	107756.0
54	97194.5
55	86633.0
56	75734.5
57	64836.0
58	61124.5
59	57413.0
60	55027.5
61	52642.0
62	45998.5
63	39355.0
64	32773.0
65	26191.0
66	21698.5
67	17206.0
68	13636.5
69	10067.0
70	8701.5
71	7336.0
72	6576.5
73	5817.0
74	4856.5
75	2692.0
76	1488.0
77	899.5
78	311.0
79	314.5
80	318.0
81	203.0
82	88.0
83	97.0
84	106.0
85	56.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1978146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.28018184475322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1868421329247	11.94709165658102
2	9.795785159363719	2.99362757094425
3	3.455906362755079	1.584206329840119
4	1.6596404521297952	1.0143843162180688
5	0.8840077693129463	0.6753899733638237
6	0.5219106660580806	0.47849339304502453
7	0.3332124170952842	0.35640824283019895
8	0.2572701595857532	0.31449078573591915
9	0.1868051257448623	0.2568974661822138
>10	1.8099481321490793	6.580400187512307
>50	0.8497686702686749	9.583110196738797
>100	2.0273103702529527	60.60964849511466
>500	0.028361522799713688	2.8216725146992205
>1k	0.003231059559461053	0.7841788711943807
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3160	0.15974553951022827	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2048	0.10353128636612262	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07921558873814168	0.0
2	0.0	0.0	0.0	0.3112510401153403	0.0
3	0.0	0.0	0.0	0.4536571112546799	0.0
4	0.0	0.0	0.0	0.761622246285158	0.0
5	0.0	0.0	0.0	1.2173014529766761	0.0
6	0.0	0.0	0.0	1.7480004003748966	0.0
7	0.0	0.0	0.0	1.980238061295779	0.0
8	0.0	0.0	0.0	2.5531482509379995	0.0
9	0.0	0.0	0.0	2.7169379813219043	0.0
10	0.0	0.0	0.0	2.975614540079448	0.0
11	0.0	0.0	0.0	3.377303798607383	0.0
12	0.0	0.0	0.0	3.688352629179039	0.0
13	0.0	0.0	0.0	3.820041594503136	0.0
14	0.0	0.0	0.0	3.8789351241010523	0.0
15	0.0	0.0	0.0	3.9620937989410288	0.0
16	0.0	0.0	0.0	4.153384027265935	0.0
17	0.0	0.0	0.0	4.376673915878808	0.0
18	0.0	0.0	0.0	4.651223923815532	0.0
19	0.0	0.0	0.0	4.794539937901449	0.0
20	0.0	0.0	0.0	4.927442160487648	0.0
21	0.0	0.0	0.0	5.132937609256344	0.0
22	0.0	0.0	0.0	5.3182121036566565	0.0
23	0.0	0.0	0.0	5.539682106376374	0.0
24	0.0	0.0	0.0	5.684666349197683	0.0
25	0.0	0.0	0.0	5.804930475303643	0.0
26	0.0	0.0	0.0	5.924436315620788	0.0
27	0.0	0.0	0.0	6.032011792860588	0.0
28	0.0	0.0	0.0	6.150051613986025	0.0
29	0.0	0.0	0.0	6.28113395067907	0.0
30	0.0	0.0	0.0	6.454427529616115	0.0
31	0.0	0.0	0.0	6.628883813429343	0.0
32	0.0	0.0	0.0	6.759460626263178	0.0
33	0.0	0.0	0.0	6.891301248745037	0.0
34	0.0	0.0	0.0	7.048165302257771	0.0
35	0.0	0.0	0.0	7.2613447136864515	0.0
36	0.0	0.0	0.0	7.425437758385883	0.0
37	5.055238592095831E-5	0.0	0.0	7.592159527153203	0.0
38	1.0110477184191662E-4	0.0	0.0	7.737345979518195	0.0
39	1.0110477184191662E-4	0.0	0.0	7.890418604086857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.034523E-4	45.0	40
GTCATCG	20	7.034523E-4	45.0	1
CTTCGAA	45	3.8562575E-10	45.0	31
CTAGAAC	20	7.034523E-4	45.0	11
AGCGTCG	25	3.89183E-5	45.0	24
GTCGCGA	20	7.034523E-4	45.0	27
CGAACCG	20	7.034523E-4	45.0	36
TCCGCGA	25	3.89183E-5	45.0	23
GTCGATC	20	7.034523E-4	45.0	33
GTCGACG	40	6.8193913E-9	45.0	1
CTCCGAT	20	7.034523E-4	45.0	38
CGACGTC	40	6.8193913E-9	45.0	18
CATGCGT	20	7.034523E-4	45.0	34
CTATCGG	35	1.2125201E-7	45.0	2
TAGACCG	50	2.1827873E-11	45.0	1
ACGCACC	40	6.8193913E-9	45.0	40
TAGGTCG	45	3.8562575E-10	45.0	2
CGTGAAC	45	3.8562575E-10	45.0	36
ATTACGA	20	7.034523E-4	45.0	41
TAGCGCG	45	3.8562575E-10	45.0	1
>>END_MODULE