FastQCFastQC Report
Thu 26 May 2016
SRR1545275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545275_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2786715
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.12433994864921602No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT253.8922408E-545.00000444
GTCGTTA502.1827873E-1145.00000410
CGTACGT253.8922408E-545.00000434
ACGACCC253.8922408E-545.00000427
CTGTCGA406.8212103E-945.043
CGACACG207.03502E-445.015
TATGCGA406.8212103E-945.019
ATCGTCC207.03502E-445.043
CCGTATC207.03502E-445.011
CTACGTA406.8212103E-945.018
CGCTAGT453.8562575E-1045.036
ACCGTAT207.03502E-445.010
CGACTTA351.2127566E-745.042
ATCGATA207.03502E-445.010
TCCGATC302.16662E-644.99999614
ATCCGCC302.16662E-644.99999618
CGCTACG302.16662E-644.99999633
CCGTTGA302.16662E-644.99999632
TACGTAC302.16662E-644.99999623
TTGTACG1100.042.9545481
Produced by FastQC (version 0.11.5)