##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545275_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2786715
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333733087165353	31.0	31.0	33.0	30.0	34.0
2	31.660777295130647	31.0	31.0	34.0	30.0	34.0
3	31.751013289841264	31.0	31.0	34.0	30.0	34.0
4	30.313541571348345	35.0	33.0	37.0	0.0	37.0
5	32.905702233633505	35.0	33.0	37.0	19.0	37.0
6	34.54430395645051	35.0	33.0	37.0	30.0	37.0
7	35.55632312597449	37.0	35.0	37.0	33.0	37.0
8	35.750879799333624	37.0	35.0	37.0	35.0	37.0
9	37.791600145691255	39.0	37.0	39.0	35.0	39.0
10	37.20508555772657	39.0	37.0	39.0	33.0	39.0
11	36.75487374919933	39.0	35.0	39.0	32.0	39.0
12	35.78851156289753	37.0	35.0	39.0	32.0	39.0
13	35.41016214431688	37.0	35.0	39.0	30.0	39.0
14	36.21383313327699	38.0	35.0	40.0	31.0	41.0
15	36.54052818461881	38.0	35.0	40.0	31.0	41.0
16	36.73167367312409	38.0	35.0	40.0	32.0	41.0
17	36.67293282592587	38.0	35.0	40.0	32.0	41.0
18	36.645112973519	38.0	35.0	40.0	31.0	41.0
19	36.63735437603056	38.0	35.0	40.0	31.0	41.0
20	36.54643693380916	38.0	35.0	40.0	31.0	41.0
21	36.37508213075252	38.0	35.0	40.0	31.0	41.0
22	36.28051881875255	38.0	35.0	40.0	31.0	41.0
23	36.23701311400699	37.0	35.0	40.0	31.0	41.0
24	36.18022438606029	37.0	35.0	40.0	31.0	41.0
25	36.069996752448674	37.0	34.0	40.0	31.0	41.0
26	35.96652007830008	37.0	34.0	40.0	30.0	41.0
27	35.9846385439487	37.0	34.0	40.0	30.0	41.0
28	36.023189310711714	37.0	35.0	40.0	30.0	41.0
29	36.07776396222793	37.0	35.0	40.0	30.0	41.0
30	36.01991484597456	37.0	35.0	40.0	30.0	41.0
31	35.882480267985784	37.0	34.0	40.0	30.0	41.0
32	35.78450648882286	37.0	34.0	40.0	30.0	41.0
33	35.676520921586885	37.0	34.0	40.0	30.0	41.0
34	35.60007356331738	37.0	34.0	40.0	30.0	41.0
35	35.47788094584484	37.0	34.0	40.0	29.0	41.0
36	35.32245134504246	36.0	34.0	40.0	29.0	41.0
37	35.25183450765507	36.0	34.0	40.0	29.0	41.0
38	35.21432331616258	36.0	34.0	40.0	29.0	41.0
39	35.17275035301421	36.0	34.0	40.0	28.0	41.0
40	35.075483499389065	36.0	34.0	40.0	28.0	41.0
41	35.052021107289406	36.0	34.0	40.0	28.0	41.0
42	35.0071277471862	36.0	34.0	40.0	28.0	41.0
43	34.952632759360036	36.0	33.0	40.0	28.0	41.0
44	34.891936563301236	35.0	34.0	40.0	27.0	41.0
45	34.793383248735516	35.0	34.0	40.0	27.0	41.0
46	34.7584367256788	35.0	34.0	40.0	27.0	41.0
47	34.69860462946516	35.0	33.0	40.0	27.0	41.0
48	34.59041487916777	35.0	33.0	40.0	27.0	41.0
49	34.5741311903083	35.0	33.0	39.0	27.0	41.0
50	34.41159501420131	35.0	33.0	39.0	27.0	41.0
51	34.32034205148356	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	10.0
15	48.0
16	121.0
17	386.0
18	907.0
19	1767.0
20	3239.0
21	5311.0
22	8369.0
23	12523.0
24	18422.0
25	25402.0
26	33767.0
27	42644.0
28	52470.0
29	64669.0
30	81425.0
31	103651.0
32	134809.0
33	196432.0
34	294262.0
35	397432.0
36	211046.0
37	270080.0
38	362096.0
39	465197.0
40	229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.064102357076344	25.84053267018694	30.841044024954112	12.254320947782604
2	27.372300360819104	26.283850339916352	32.509962446823586	13.833886852440958
3	28.728664395174963	25.85258987732868	31.569679712493027	13.849066015003327
4	22.049725214096167	39.21028881676095	26.280620730860527	12.45936523828235
5	23.14589041218783	32.78318737294628	29.70956843451878	14.36135378034711
6	22.302962448617816	40.40664366467328	26.447627403591685	10.842766483117218
7	91.00876838858656	3.4756334967874363	3.962084389684629	1.5535137249413735
8	92.96411724916254	2.0173573544477996	3.252467511030012	1.766057885359644
9	89.03827624999327	4.031700407110163	4.62799389245043	2.3020294504461343
10	55.35808290406447	27.293928514397773	8.928505426640328	8.419483154897433
11	45.791370843448284	20.15372939105721	21.072840243799597	12.9820595216949
12	41.990695137464726	22.154328662959795	21.46595543498348	14.389020764592
13	22.12892240505398	40.272112505225685	22.412230888339852	15.186734201380478
14	15.27881394401652	41.450130350609946	26.970178148823976	16.300877556549555
15	14.096489953224495	24.464288597865227	46.63142804341312	14.807793405497153
16	18.05466292749707	20.32181260014031	43.21159501420131	18.41192945816131
17	18.418065715367376	21.102409108932918	26.13467828608236	34.34484688961735
18	23.386855132297345	24.927773381921007	32.06348693712848	19.621884548653163
19	30.973206804427434	25.776622295426694	23.276115426227655	19.974055473918213
20	33.39846378262578	24.985726922200513	23.429844817284867	18.18596447788884
21	23.843378314610572	28.39709119877705	27.44162930188412	20.317901184728253
22	24.23096728585449	25.202577228026545	24.973920906874223	25.592534579244735
23	21.053390820374528	30.811475159820795	25.100808658223034	23.034325361581647
24	20.866037610591682	24.959315897032887	35.98749782449946	18.187148667875977
25	19.094848235287785	27.707749088084	32.92112756417502	20.27627511245319
26	17.965705140281656	36.70131319492664	24.970655413273334	20.362326251518365
27	18.44483558598565	33.78052653392973	29.06820396057724	18.706433919507376
28	15.833337818901466	29.64160310616622	36.9110224762848	17.614036598647512
29	16.595453786985754	25.891704031449216	36.01326292785592	21.49957925370912
30	20.462408247703838	30.06317474158642	30.033713530088296	19.440703480621448
31	29.204744654548453	27.382563340707605	23.67945771275498	19.733234291988953
32	30.996208797813914	26.676714339284786	24.554107614162195	17.772969248739106
33	27.849851886540243	27.43427297014585	24.350857550915684	20.365017592398218
34	20.196037269688503	28.265394918389575	27.158428472233435	24.380139339688487
35	20.06258264659285	26.145407765056706	30.488693676963734	23.303315911386704
36	30.614899621956315	24.50523286378406	26.25227194025941	18.62759557400021
37	21.646921195744813	31.230929607082174	27.744889592225974	19.377259604947046
38	21.082852031872655	32.73459969892867	23.5337305752472	22.648817693951482
39	21.674839371805156	30.540833920942763	26.39975742047536	21.384569286776724
40	25.024195154509883	24.895549060452897	25.696671529022524	24.383584256014696
41	18.90125111466368	23.729660191300507	27.340542538436836	30.028546155598978
42	22.381154872313818	25.635631917867453	24.774259298134183	27.208953911684546
43	22.033218323366402	26.4601152252742	26.499085841214477	25.00758061014492
44	20.70771499776619	30.02653662107535	27.64627886238815	21.61946951877031
45	18.653217139176416	34.57770170254224	23.036514318830594	23.73256683945075
46	21.96948737133148	32.36412765568061	25.475694500514045	20.190690472473865
47	21.36153140884518	27.34829360017081	27.83402680216671	23.4561481888173
48	22.595457375440258	23.374152003344438	31.14502200619726	22.885368615018038
49	20.97699262393176	23.65039123125257	31.713074354571603	23.659541790244067
50	19.58381104633951	32.17218122412949	26.021247239132816	22.222760490398194
51	18.494033297269365	33.81964068805027	24.00180858107126	23.684517433609106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	459.0
1	880.0
2	1301.0
3	4118.0
4	6935.0
5	5056.5
6	3178.0
7	3598.0
8	4018.0
9	4318.5
10	4619.0
11	4751.0
12	4883.0
13	5054.5
14	5226.0
15	5021.5
16	4817.0
17	5069.0
18	5321.0
19	5744.0
20	6167.0
21	8160.0
22	10153.0
23	10473.0
24	10793.0
25	13254.5
26	20401.0
27	25086.0
28	29885.5
29	34685.0
30	40929.5
31	47174.0
32	56203.0
33	65232.0
34	73517.0
35	81802.0
36	87468.0
37	93134.0
38	115780.0
39	138426.0
40	184608.0
41	230790.0
42	259112.0
43	287434.0
44	283472.5
45	279511.0
46	263121.5
47	246732.0
48	233957.5
49	221183.0
50	204167.5
51	187152.0
52	171671.5
53	156191.0
54	143002.5
55	129814.0
56	119564.0
57	109314.0
58	104969.0
59	100624.0
60	93633.5
61	86643.0
62	73350.0
63	60057.0
64	52657.0
65	45257.0
66	37522.5
67	29788.0
68	23270.5
69	16753.0
70	15169.5
71	13586.0
72	9981.0
73	6376.0
74	6204.0
75	3924.0
76	1816.0
77	1670.0
78	1524.0
79	1087.5
80	651.0
81	469.5
82	288.0
83	169.0
84	50.0
85	31.5
86	13.0
87	10.0
88	7.0
89	5.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2786715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.056907459250606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93706844328396	12.353713881798408
2	9.792564935026217	3.144766179008368
3	3.46762584242493	1.670380417653691
4	1.658558215706074	1.0652526314148898
5	0.9393364911222826	0.7541419555523835
6	0.5879200459630621	0.5664106662880352
7	0.41887504385863716	0.4708086471359373
8	0.32551603061197304	0.4181424624031231
9	0.26540083607093357	0.3835364997958849
>10	2.59691699177381	9.860110730232524
>50	1.1216132652096669	13.243305705486078
>100	1.8766383056847715	54.61151807344275
>500	0.010744578440782571	1.0922774126362909
>1k	0.0012209748228162016	0.3656347371516463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3465	0.12433994864921602	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06075253479455201	0.0
2	0.0	0.0	0.0	0.2314194311223071	0.0
3	0.0	0.0	0.0	0.33365450001166247	0.0
4	0.0	0.0	0.0	0.5247038179361722	0.0
5	0.0	0.0	0.0	0.8981901629696614	0.0
6	0.0	0.0	0.0	1.2560667309000024	0.0
7	0.0	0.0	0.0	1.448120816086324	0.0
8	0.0	0.0	0.0	1.9088424901721202	0.0
9	0.0	0.0	0.0	2.0468903350360548	0.0
10	0.0	0.0	0.0	2.285271367900916	0.0
11	0.0	0.0	0.0	2.6610543238185462	0.0
12	0.0	0.0	0.0	2.9556664387998053	0.0
13	0.0	0.0	0.0	3.083164227414716	0.0
14	0.0	0.0	0.0	3.1363451232006145	0.0
15	0.0	0.0	0.0	3.212635666008185	0.0
16	0.0	0.0	0.0	3.3979434567223414	0.0
17	0.0	0.0	0.0	3.6288963887588075	0.0
18	0.0	0.0	0.0	3.875423213353357	0.0
19	0.0	0.0	0.0	4.042178694269059	0.0
20	0.0	0.0	0.0	4.197056390768342	0.0
21	0.0	0.0	0.0	4.41781811200643	0.0
22	0.0	0.0	0.0	4.650924116746779	0.0
23	0.0	0.0	0.0	4.89906574586924	0.0
24	3.5884545064708806E-5	0.0	0.0	5.078452586647719	0.0
25	3.5884545064708806E-5	0.0	0.0	5.229741828640532	0.0
26	3.5884545064708806E-5	0.0	0.0	5.378160307028168	0.0
27	3.5884545064708806E-5	0.0	0.0	5.535155191686268	0.0
28	3.5884545064708806E-5	0.0	0.0	5.699147562631988	0.0
29	3.5884545064708806E-5	0.0	0.0	5.879754477942667	0.0
30	7.176909012941761E-5	0.0	0.0	6.093267521077685	0.0
31	7.176909012941761E-5	0.0	0.0	6.295118087066672	0.0
32	1.0765363519412642E-4	0.0	0.0	6.474217851484633	0.0
33	1.0765363519412642E-4	0.0	0.0	6.652133425915459	0.0
34	1.0765363519412642E-4	0.0	0.0	6.8518668037456285	0.0
35	1.0765363519412642E-4	0.0	0.0	7.081348469434442	0.0
36	1.0765363519412642E-4	0.0	0.0	7.283378458148753	0.0
37	1.0765363519412642E-4	0.0	0.0	7.5016282612323115	0.0
38	1.0765363519412642E-4	0.0	0.0	7.729208046032695	0.0
39	1.0765363519412642E-4	0.0	0.0	8.047970459842503	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	25	3.8922408E-5	45.000004	44
GTCGTTA	50	2.1827873E-11	45.000004	10
CGTACGT	25	3.8922408E-5	45.000004	34
ACGACCC	25	3.8922408E-5	45.000004	27
CTGTCGA	40	6.8212103E-9	45.0	43
CGACACG	20	7.03502E-4	45.0	15
TATGCGA	40	6.8212103E-9	45.0	19
ATCGTCC	20	7.03502E-4	45.0	43
CCGTATC	20	7.03502E-4	45.0	11
CTACGTA	40	6.8212103E-9	45.0	18
CGCTAGT	45	3.8562575E-10	45.0	36
ACCGTAT	20	7.03502E-4	45.0	10
CGACTTA	35	1.2127566E-7	45.0	42
ATCGATA	20	7.03502E-4	45.0	10
TCCGATC	30	2.16662E-6	44.999996	14
ATCCGCC	30	2.16662E-6	44.999996	18
CGCTACG	30	2.16662E-6	44.999996	33
CCGTTGA	30	2.16662E-6	44.999996	32
TACGTAC	30	2.16662E-6	44.999996	23
TTGTACG	110	0.0	42.954548	1
>>END_MODULE