FastQCFastQC Report
Thu 26 May 2016
SRR1545274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545274_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3170271
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38890.12267090100499295No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.10835035869173329No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32830.10355581589081818No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA207.0351665E-445.018
GTCGACG253.8923645E-545.011
CGCATCG207.0351665E-445.021
ATTACGC253.8923645E-545.031
ATAGCCG207.0351665E-445.010
TCGATCG207.0351665E-445.01
ATCCGAT453.8562575E-1045.045
AATGCGT207.0351665E-445.037
GTCGTAC253.8923645E-545.032
GAACGTA453.8562575E-1045.011
TTGCGAA207.0351665E-445.028
AACGACC253.8923645E-545.020
ACGTCGA207.0351665E-445.043
ACCCGTA207.0351665E-445.033
GTACGTA406.8212103E-945.035
AGTCGAC253.8923645E-545.010
GTACGAC253.8923645E-545.019
CTTACGC207.0351665E-445.011
TCTACGT302.1667147E-644.99999620
GTTCGGA302.1667147E-644.99999642